Method for producing anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus

A technology based on anticancer activity and Aspergillus fumigatus, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low CHA yield and low yield, and achieve optimal fermentation and culture conditions, rapid growth, and methods simple effect

Pending Publication Date: 2020-11-20
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Technical problem to be solved: This application mainly proposes a method for producing the anticancer active compound CHA by using the sy

Method used

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  • Method for producing anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus
  • Method for producing anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus
  • Method for producing anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus

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Embodiment 1

[0035] A method for producing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:

[0036] The first step is to prepare the fermentation medium: accurately weigh 36 g of glucose, 24 g of mannitol, 3.5 g of sodium nitrate, 1.5 g of potassium dihydrogen phosphate, 0.02 g of ferrous sulfate heptahydrate, 0.7 g of magnesium sulfate heptahydrate, glutamic acid Sodium 3 g, sodium tartrate 1.5 g, dissolved in tap water, and dilute to 1000 mL;

[0037]The second step, the fermentation operation process: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, use an inoculation needle to spot the sample on the center of the solid plate medium PDA, place it in a constant temperature incubator at 28°C, and activate it for 7 days to obtain Fresh solid plate medium, and then stored in a refrigerator at 4°C. Use an inoculation shovel to dig out a 5×5 mm agar block from the fresh solid plate medium of Aspergi...

Embodiment 2

[0041] A method for producing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:

[0042] The first step is to prepare the fermentation medium: accurately weigh 20 g of maltose, 10 g of glucose, 20 g of mannitol, 10 g of sodium glutamate, 3 g of yeast extract, 1 g of corn steep liquor, 0.5 g of potassium dihydrogen phosphate, and seven Water magnesium sulfate 0.3 g, dissolved in tap water, and dilute to 1000 mL;

[0043] The second step, the fermentation operation process: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, use an inoculation needle to spot the sample on the center of the solid plate medium PDA, place it in a constant temperature incubator at 28°C, and activate it for 7 days to obtain Fresh solid plate medium, and then stored in a refrigerator at 4°C. Use an inoculation shovel to dig out a 5×5 mm agar block from the fresh solid plate medium of Aspergillus fumigatus symbios...

Embodiment 3

[0047] A method for producing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:

[0048] The first step is to prepare the fermentation medium: accurately weigh 20 g of malt extract, 20 g of glucose, and 1 g of peptone, dissolve in tap water, and set the volume to 1000 mL;

[0049] The second step, the fermentation operation process: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, use an inoculation needle to spot the sample on the center of the solid plate medium PDA, place it in a constant temperature incubator at 28°C, and activate it for 7 days to obtain Fresh solid plate medium, and then stored in a refrigerator at 4°C. Use an inoculation shovel to dig out a 5×5 mm agar block from the fresh solid plate medium of Aspergillus fumigatus symbiosis, inoculate it into a 1000 mL shake flask containing 400 mL of seed medium PDB, and incubate at 28°C, 150 The rpm shaker was fermented and c...

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Abstract

The invention discloses a method for producing an anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus, and relates to the field of microbial culture mediums, specifically to a method used for fermenting and culturing sea crab symbiotic Aspergillus fumigatus CY018 to produce an alkaloid anticancer active compound Chaetominine, namely CHA.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, in particular to a method for producing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs. Background technique [0002] The ocean occupies nearly 71% of the earth's surface. Due to the high-salt, high-pressure, low-temperature, and low-oxygen environments, various marine organisms must co-evolve with their symbiotic bacteria for a long time and establish a stable mutualistic symbiotic relationship, which enriches the diversity of marine organisms. nature, and can synthesize many marine natural products with unique structures and significant biological activities, attracting many experts and scholars to obtain natural products from the ocean. The number of new natural products discovered by scientific researchers over the years has been increasing year by year, among which natural products with anticancer activity account for 63%, ...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12N1/14C12R1/68
CPCC12P7/62C12N1/14
Inventor 刘长青黄正洋陈吉杰汤雯婷李朝霞梁慧星仇明
Owner YANCHENG INST OF TECH
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