Gel preparation system

Abstract

The invention provides a gel preparation system. The gel preparation system comprises a controller, a liquid guide tank and a plate tank, wherein the controller is signally connected with a touch screen, a second drive motor, a first drive motor, a No. 4 pump, a No. 1 pump, a No. 3 pump, a shunt pump and a No. 4 syringe respectively, the lower end of the liquid guide tank is correspondingly provided with a collector, the upper end of the liquid guide tank is provided with a first injection needle, the first injection needle is connected with a No. 1 bottle through a pipeline of the No. 1 pump,one of liquids for gel preparation is arranged in the No. 1 bottle, the gel preparation system further comprises at least one second injection needle, the second injection needle is embedded in a second sealing gasket and the two move relative to each other, the second injection needle is connected with a solution supply bottle through a pipeline of the No. 4 pump, and the plate tank is a gel forming mold. The system can realize liquid addition and automatic mixing of trace reagents, has high precision, has high degree of automation, avoids the contact between humans and toxic reagents, improves safety system, and improves work efficiency.

Description

technical field [0001] The invention belongs to the field of gel production, in particular to a gel preparation system. Background technique [0002] Polyacrylamide gel electrophoresis (English: polyacrylamide gel electrophoresis, referred to as PAGE) uses polyacrylamide gel as a supporting medium. Polyacrylamide gel is formed by the polymerization of monomeric acrylamide (CH2=CHCONH2Acrylamide) and methylenebisacrylamide (CH2(NHCOHC=CH2)2N, N'-methylenebisacrylamide). This polymerization process requires a catalyst persulfate Ammonium (AP) and accelerator tetramethylethylenediamine (TEMED) free radical catalysis is accomplished. Polyacrylamide gel electrophoresis PAGE is divided into two categories: continuous system and discontinuous system according to whether it has a concentration effect. In the continuous system electrophoresis system, the pH value of the buffer and the gel concentration are the same. Charge and molecular sieve effects. In a discontinuous system, du...

AI Technical Summary

Problems solved by technology

[0003] The preparation process of polyacrylamide gel is a mixing process of various reagents such as acrylamide, ammonium persulfate, tetramethylethylenediamine, SDS, Tris-Hcl, water, etc., depending on the type and molecular weight of the separated substance, different Gel concentration, and for discontinuous gels, stacking gels and separating gels require very different reagent doses. During manual operation, it is necessary to always pay attention to the ratio of reagents. Accidental addition of reagents may occur occasionally. In addition, in the process of gel preparation, acrylamide and tetramethylethylenediamine have strong neurotoxicity and are very harmful to experimenters.

Therefore, the entire polyacrylamide gel preparation process is simple and cumbersome, time-consuming, and labor-intensive, which is potentially harmful to researchers, and human errors often affect the overall progress of the experiment.

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