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Nanoparticle cluster assembling method with micro magnetic beads as growth template and DNA frame as guide carrier

A nanoparticle and growth template technology, which is applied in nanotechnology, nanotechnology, nanostructure manufacturing, etc., can solve the problems of low yield, multiple purifications, and many by-products, and achieve high yield, rapid preparation, and short time. Effect

Active Publication Date: 2020-11-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are defects in the existing assembly method, such as many by-products, low yield, and the need for multiple purifications
[0004] At present, there is a lack of a high-yield nanoparticle cluster assembly method using micro-magnetic beads as growth templates and DNA frameworks as guide carriers.

Method used

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  • Nanoparticle cluster assembling method with micro magnetic beads as growth template and DNA frame as guide carrier
  • Nanoparticle cluster assembling method with micro magnetic beads as growth template and DNA frame as guide carrier
  • Nanoparticle cluster assembling method with micro magnetic beads as growth template and DNA frame as guide carrier

Examples

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Effect test

Embodiment 1

[0030] A method for assembling nanoparticle clusters using micro-magnetic beads as a growth template and a DNA framework as a guide carrier of the present invention comprises the following steps:

[0031] (1) The octahedral DNA origami framework was selected, mixed with the modified 10 nm gold balls at a ratio of 1:1.3 and quenched at a constant speed for 10 hours, and purified by agarose gel electrophoresis to obtain a DNA origami complex carrying gold balls Objects, that is, growth units assembled by nanoparticle clusters; the DNA origami framework is an octahedral DNA origami framework, and the nucleotide sequences of the octahedron DNA origami framework are as SEQ ID NO: 1, 2, 3, 4... The nucleotide sequence shown at 120.

[0032] (2) Mix the first layer of growth units with the DNA-modified micro-magnetic bead solution, and react at room temperature for 4 hours. After the reaction, use a magnet to wash with buffer three times to remove unconnected growth units, and then a...

Embodiment 2

[0034] The difference between Example 2 and Example 1 is that in step (1), the octahedral DNA origami framework is selected, mixed with the modified 10nm gold balls at a ratio of 1:1.3, and quenched at a constant speed for 10 hours, and passed through agarose After gel electrophoresis purification, DNA origami complexes carrying gold spheres, i.e. growth units assembled by nanoparticle clusters, were obtained;

[0035] In step (2), mix the first layer of growth units with the DNA-modified micro-magnetic bead solution, and react at room temperature for 8 hours. After the reaction, use a magnet to wash with buffer three times to remove unconnected growth units, and then Add the second layer of growth unit, and the constant temperature reaction time on the metal mixer is 12 hours, and the rotation speed is 1000 rpm. After the reaction is completed, it is also cleaned with a magnet, and then the third layer of growth unit is added, and the constant temperature reaction time is 12 h...

Embodiment 3

[0037] The difference between embodiment 3 and embodiment 1 is:

[0038] In step (1), the octahedral DNA origami framework was selected, mixed with the modified 10 nm gold balls at a ratio of 1:1.3 and quenched at a constant speed for 10 hours, and purified by agarose gel electrophoresis to obtain gold balls carrying DNA origami complexes, growth units assembled by nanoparticle clusters;

[0039]In step (2), mix the first layer of growth units with the DNA-modified micro-magnetic bead solution, and react at room temperature for 8 hours. After the reaction, use a magnet to wash with buffer three times to remove unconnected growth units. Then add the second layer of growth unit, the constant temperature reaction time is 12 hours on the metal mixer, and the rotation speed is 1000 rpm. After the reaction is completed, it is also cleaned with a magnet, and then the third layer of growth unit is added, and the constant temperature reaction time is 12 hours. , the rotating speed is ...

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Abstract

The invention discloses a nanoparticle cluster assembling method with micro magnetic beads as a growth template and a DNA frame as a guide carrier. The method comprises the steps that firstly, the micro magnetic beads modified with streptavidin are combined with DNA, and the micro magnetic beads are connected with the designed DNA origami frame according to the base complementary pairing principleof DNA, so that the nanoparticle cluster assembling position is fixed. A composite structure composed of the DNA origami frame and nanoparticles serves as a growth unit, or the nanoparticles directlyserve as a growth unit. After the growth units are designed, DNA sequences connected between the growth units are designed, and assembling of nanoparticle clusters is guided through DNA sequence complementation. By means of the fixed-point position growth and DNA frame guide assembling mode, a high-precision and high-yield target assembly can be obtained. The method is high in yield, controllableand easy to design. The whole preparation process is short in needed time, free of pollution, easy to operate and capable of achieving rapid preparation.

Description

technical field [0001] The invention relates to the technical field of nanostructure materials, in particular to a method for assembling nanoparticle clusters using micro-magnetic beads as growth templates and DNA frameworks as guiding carriers. Background technique [0002] DNA nanotechnology provides a new method for the nanoscale assembly and construction of inorganic nanomaterials, as well as the ordered assembly of proteins or other biomacromolecules. The concept of DNA nanotechnology was first proposed by Professor Seeman of New York University in 1982, aiming to first construct a DNA crystal structure (unit cell) through the excellent addressability of DNA, the principle of complementary base pairing, and designability. There is enough space in the unit to embed the target assembly unit—nanomaterial), and a simple and effective universal method for constructing nanoscale assembly of inorganic nanomaterials is realized through the connection of DNA, which provides new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/00B82B3/00
CPCB82B3/0061B22F1/148
Inventor 王利辉解晓琳田野
Owner NANJING UNIV