Antibody combined with human LAG-3 protein as well as coding gene and application thereof

Abstract

The invention discloses an antibody combined with human LAG3 protein as well as a coding gene and application of the antibody. The antibody combined with the human LAG-3 protein is a novel antibody molecular sequence different from that of an existing LAG-3 antibody, has an antigen binding site specifically combined with the human LAG-3 protein, and shows good biological blocking activity. Meanwhile, the antibody disclosed by the invention can compete for an antigen epitope with BMS986016, not only can inhibit the BMS986016 from being combined with an antigen, but also can promote the BMS986016 combined with the antigen to be partially separated; and a result shows that a binding site of the antibody and the human LAG-3 protein is different from a binding site of the BMS986016 with the antigen, an antigen configuration can be changed by combining the antibody combined with the human LAG-3 protein, and it is speculated that a blocking effect thereof on an inhibition pathway of LAG-3 onT cells is better than that of BMS986016.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to an antibody combined with human LAG-3 protein, its coded amino acid sequence and application. Background technique [0002] Under normal circumstances, the human immune system can recognize and eliminate tumor cells in the tumor microenvironment. The "tumor-immune cycle" proposed by Chen He explained the seven steps of tumor generation and the elimination of tumor cells by the human immune system: (1) Tumor antigens (2) Tumor antigen presentation; (3) Initiation and activation of effector T cells; (4) T cell migration to tumor tissue; (5) T cell infiltration in tumor tissue; (6) T cell recognition of tumor cells; (7) ) to remove tumor cells. [0003] However, in order to survive and grow, tumor cells can adopt different strategies to suppress the immune system of the human body, cause abnormalities and protect themselves from being killed and eliminated, th...

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Problems solved by technology

[0003] However, in order to survive and grow, tumor cells can adopt different strategies to suppress the immune system of the human body, cause abnormalities and protect themselves from being killed and eliminated, thus surviving at various stages of the anti-tumor immune response

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