Unlock instant, AI-driven research and patent intelligence for your innovation.

Plant TMK1 gene and application thereof in sclerotinia sclerotiorum resistance

A sclerotinia and gene technology, applied in the field of plant disease resistance of the plant receptor transmembrane protein TMK1 gene and its encoded protein, can solve the problems affecting the national economy and people's livelihood such as food security, crop disasters, and agricultural production losses

Active Publication Date: 2020-12-04
FUJIAN AGRI & FORESTRY UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Almost all plants will be invaded by pathogenic fungi and bacteria during the growth and development process. A wide variety of pathogenic bacteria seriously affect the growth of plants, often causing damage to large areas of crops, causing huge losses to agricultural production, and seriously affecting the national economy and people's livelihood and national food. safety

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant TMK1 gene and application thereof in sclerotinia sclerotiorum resistance
  • Plant TMK1 gene and application thereof in sclerotinia sclerotiorum resistance
  • Plant TMK1 gene and application thereof in sclerotinia sclerotiorum resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 After inoculating mycelia of S. sclerotiorum oxalic acid deletion mutant (S. sclerotiorum A2), tmk1 Tolerance phenotype analysis of mutant (SALK_016360).

[0037] Detached Arabidopsis leaves grown for about 4 weeks were inoculated with S. sclerotiorum A2 hyphae. 36 hours after inoculation, the phenotype observation of leaf disease and the statistical analysis of leaf plaque area were carried out. Taking leaf diseased spots as the object of investigation, tmk1 The overall disease state of the mutant is weaker than that of the wild type, and this phenotype can be complemented by TMK1, which is consistent with the results of trypan blue staining ( figure 1 ).

[0038] tmk1 The area of ​​dead cells in the mutant infected by S. sclerotiorum was significantly smaller than that of the wild type. The statistical results of leaf damage area also show that, tmk1 Mutants had enhanced resistance to the fungus S. sclerotiorum A2 ( figure 2 ).

Embodiment 2

[0039] Example 2 Construction of pTMK1:TMK1 vector

[0040] For further verification tmk1 The phenotype of the mutant's resistance to Sclerotinia thaliana, we cloned the TMK1 gene (shown in SEQ ID NO.1) from the wild-type Arabidopsis genome, including its upstream 3000bp promoter sequence (see image 3 ); and the amplified gene sequence was connected to the expression vector pCAMBIA1300 containing the FLAG tag, and sequenced for final detection and verification, and finally the pTMK1:TMK1-FLAG vector was constructed.

[0041] The primer sequences used are:

[0042] Upstream primer F: 5-CTTATTTTTTGCTTTTTGATTGTTT-3 (SEQ ID NO.3),

[0043] Downstream primer R: 5-TCGTCCATCTACTGAAGTGAATG-3 (SEQ ID NO.4).

Embodiment 3

[0044] Example 3 Construction and detection of transgenic plants

[0045] Contain in embodiment 2 gTMK1-FLAG The pCAMBIA1300 vector of the gene was transformed into Agrobacterium GV3101, and then transformed by Arabidopsis flower dipping method tmk1 Homozygous mutants, obtained by resistance selection tmk1 Complementary lines of mutants. The specific method is: inoculate the Agrobacterium containing the target vector in 80 mL of LB liquid medium (containing 50 ug / mL Kan, 50 ug / mL Rif), shake and cultivate overnight at 28 ° C, and wait until the OD 600 When it is about 1.6, centrifuge at 5000rpm for 10min; remove the supernatant, add 30mL 5wt.% sucrose to suspend the cells, and add 10uL Silwet to mix well to obtain the transformation liquid. Soak the Arabidopsis flower buds in the transformation solution for 1 min, take them out and put them in a black fresh-keeping bag and place them in a dark place for 12 hours; remove the fresh-keeping bags the next day, and put the pl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a plant TMK1 gene and application thereof in sclerotinia sclerotiorum resistance. The gene is an arabidopsis TMK1 gene, the nucleotide sequence is SEQ ID NO. 1, and the amino acid sequence of protein coded by the gene is shown as SEQ ID NO. 2. The TMK1 gene plays an important role in plant response to sclerotinia sclerotiorum infection, provides gene resources for cultivating new varieties of disease-resistant plants, and has important application value in agricultural production.

Description

technical field [0001] The invention relates to the application of a plant receptor transmembrane protein TMK1 gene and its encoded protein in plant disease resistance, in particular to the application of the plant TMK1 gene and its anti-sclerotinia. Background technique [0002] Almost all plants will be invaded by pathogenic fungi and bacteria during the growth and development process. A wide variety of pathogenic bacteria seriously affect the growth of plants, often causing damage to large areas of crops, causing huge losses to agricultural production, and seriously affecting the national economy and people's livelihood and national food. safety. For this reason, it is of great practical significance to study the response mechanism of plant disease resistance process for screening and optimizing new varieties of crop disease resistance. [0003] Sclerotinia sclerotiorum is a necrotrophic pathogenic fungus with a wide range of hosts and strong invasion of hosts. Scleroti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/84A01H5/00A01H6/20
CPCC12N9/12C12N15/8282
Inventor 徐通达何军黄荣峰
Owner FUJIAN AGRI & FORESTRY UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com