In-vitro semi-dynamic simulation digestion method and device for aquatic products

A technology for simulating digestion and aquatic products. It is applied in the direction of measuring devices, material inspection products, and food testing. It can solve the problems of changing enzyme activity, affecting the digestion process, and unreported protein in aquatic products. It achieves good repeatability and simple maintenance. , easy to promote the effect

Inactive Publication Date: 2020-12-04
CHINA AGRI UNIV
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study published by Laura Sams and others abroad in Food&Function (2016), it is proposed that the pH value of the human stomach does not remain static after eating, but first rises from 1.0-1.5 to 5-7, then gradually decreases after 3 hours and then returns to 1.0- 1.5. Obviously, the change of pH value will obviously cha...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro semi-dynamic simulation digestion method and device for aquatic products
  • In-vitro semi-dynamic simulation digestion method and device for aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The temperature control system is connected with the temperature sensor, heating device, heating fan and cooling fan, the overturn control system is connected with the motor, the above two control systems are connected with the display regulator, the operation and setting are more convenient, and the main switch is turned on after plugging in the power supply You can set up and run. According to the information collected from the sensor and the target parameter setting, the temperature control system can automatically adjust the operation status of the water heating device, heating fan and cooling to achieve the final required condition setting. The same is true for the overturn control system, which can achieve the required overturning mixing speed and steering by monitoring and adjusting the operation status of the overturning motor. The turning motor is connected by connecting fasteners and drives the rotation of the main bearing. The supporting bearing on the main b...

Embodiment 2

[0090] The oysters were steamed on high heat for 8 minutes, and then the shells were removed. The edible part was processed by a 400W cooking machine for 4 minutes and then turned into mince. Weighed 5g and put them in a centrifuge tube with a cover, and clamped them with the pre-prepared simulated digestive juice and calcium ion solution. On the perforated plastic floating board, keep warm in a 37°C constant temperature water bath together, and the constant temperature digestion chamber is preheated in advance to reach a stable temperature of 37°C.

[0091] Oral simulation stage: add 5mL of oral simulated digestive juice for every 5g of pretreated aquatic products, the total volume of the system is 10mL, and 7.55mmol / L KCl, 1.85mmol / L KH 2 PO 4 , 6.26mmol / LNaHCO 3 , 0.54mmol / L NaCl, 0.075mmol / L MgCl 2 (H 2 O) 6 , 0.03mmol / L (NH 4 ) 2 CO 3 , 0.75mmol / LCaCl 2 (H 2 O) 2 , 75U / mL α-amylase; the centrifuge tubes were clamped and set in each hole of the rotating panel wit...

Embodiment 3

[0097] In the stomach and intestine simulation stage in Example 2, an enzyme inhibitor was finally added to terminate the reaction, and the supernatant was taken out by centrifugation (20,000 rpm, 12 min) at 4°C, vacuum freeze-dried, sealed and stored for later use, and other steps were the same as in Example 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an in-vitro semi-dynamic simulation digestion method and device for aquatic products, and belongs to the technical field of in-vitro simulation digestion. The method comprisesthe following steps of: oral cavity simulation, specifically, adding an oral cavity simulation digestive juice and a CaCl2 (H2O) 2 solution into an aquatic product, and incubating to obtain an oral cavity simulation digested product; stomach simulation, specifically, adding a stomach simulation digestive juice into an oral cavity simulation digested product, and adding a hydrochloric acid solutionin batches until the pH value is 2.0-1.2, supplementing 20mL of the system with water, adding a CaCl2 (H2O)2 solution for incubation to obtain a stomach simulated digested product; and intestine simulation, specifically, adding intestine simulation digestive juice into the stomach simulation digested product, adjusting the pH value to 6.9-7.4 by adopting sodium hydroxide, adding water to supplement the system to 40 mL, and adding a CaCl2 (H2O) 2 solution for incubation to obtain an intestine simulation digested product. The simulated digestive juices are more scientific and comprehensive in formula, the pH value of the digestion condition is finer, the simulated digestion stage is not unchanged. Therefore, the method and device can better fit the digestion condition of a human body.

Description

technical field [0001] The invention belongs to the technical field of in vitro simulated digestion, and in particular relates to a method and device for in vitro semi-dynamic simulated digestion of aquatic products. Background technique [0002] my country is a big country in aquatic products. Since the 1990s, the output of my country's fishery products has been ranked first in the world for many years. In 2018, the total output of aquatic products in the country was 64.5766 million tons, and the national per capita share has reached 46.28 kg. Common aquatic products include fish, shrimp, crab, shellfish and other products, which contain high-quality protein and unsaturated fatty acids, rich in vitamins and less carbohydrates. Compared with poultry and livestock products, the fat content is lower and more It is easy to digest and has high nutritional value. With the enhancement of people's health awareness and the improvement of residents' income in our country, the aquati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/02G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N33/02G01N2030/062
Inventor 洪惠冯淳淞焦亚丹罗永康
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap