Tetraploid rice breeding method

A tetraploid and rice seed technology, applied in the field of agricultural breeding, can solve the problems of small number of grains per panicle, low yield per unit area, and few effective panicles

Inactive Publication Date: 2020-12-08
WUHAN POLYPLOID BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a tetraploid rice breeding method to solve the technical problems in the prior art of tetraploid rice with poor rice quality, few grains per panicle, few effective panicles and low yield per unit area

Method used

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  • Tetraploid rice breeding method

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Experimental program
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Effect test

Embodiment approach

[0060] The said hybrid F2 generation seeds are stripped and weighed for selection, comprising:

[0061] Using the pollen of the small long-grain fertile plant to pollinate the female flower of the small long-grain sterile plant to obtain artificial backcross BC1 generation seeds with a thousand-grain weight of 15-15.5 grams and an aspect ratio > 3;

[0062] Planting the artificial backcross BC1 generation seeds to obtain backcross BC2 generation seeds;

[0063] Selfing the small long-grain fertile plant to obtain selfed S1 generation seeds with a thousand-grain weight of 16.5-17.0 grams and an aspect ratio > 3;

[0064] Planting and selfing the self-bred S1 generation seeds to obtain self-bred S2 generation seeds;

[0065] Rice peeling and weighing selection were performed on the backcross BC2 generation seeds, the self-crossing S2 generation seeds and the hybrid F2 generation seeds.

[0066] As an implementation manner of the embodiment of the present invention, the doublin...

Embodiment 1

[0079] On August 20, 2016, at the Xiangyang base, a small long-grained sterile plant was found in the F2 generation segregation population of cultivated rice subspecies (indica-japonica cross) in the selection nursery. Long-grained fertile plants. Then the male flowers of the fertile plants were used to pollinate the female flowers of the sterile plants to obtain backcross BC1 generation seeds; at the same time, an excellent indica rice restorer line containing blast resistance gene and an excellent japonica rice restorer line containing blast resistance gene were selected respectively The female flowers of the sterile plants were pollinated, and after fertilization and maturity, two hybrid F1 seeds, the indica-japonica hybrid seeds and the japonica-type indica-japonica hybrid seeds, were harvested; and the fertile plants were selfed to obtain selfed S1 seeds. Among them, the thousand-grain weight of the hybrid seeds obtained by pollinating the sterile plants with the indica r...

Embodiment 2

[0093] At the beginning of May, 2017, 20 parts of plant seeds were selected from the remaining 270 parts of plant seeds after 30 parts of plant seeds were screened out from Example 1, and 20 parts of plant seeds were selected for processing, and the seed dormancy (post-ripening effect) was broken by freezing, and the treatment time was 15 days. After the dormancy is broken, the seeds have good permeability, strong water absorption and high germination rate.

[0094] On May 16, 2017, the seeds of 20 plants that broke dormancy will be soaked to accelerate germination and planted in the field. By July 20, the young ears of the planted plants will be harvested when they have developed to the 3-4 stage, and the second double material will be obtained. .

[0095] From July 20th to 30th, 2017, the second doubling material was sent to the laboratory in 3 batches for doubling the young ears, and tetraploid material was obtained.

[0096] The doubling method is as follows: young ears a...

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Abstract

The invention provides a tetraploid rice breeding method. The method comprises the following steps of: selecting small long-grain fertile plants and small long-grain sterile plants from a hybrid and improved F2 generation segregation population of indica rice and japonica rice; pollinating female flowers of the sterile plants with indica rice restorer plants to obtain indica-biased type indica-japonica hybrid seeds; pollinating female flowers of the sterile plants with japonica rice restorer plants to obtain japonica-biased type indica-japonica hybrid seeds; respectively planting and selfing the indica-biased type indica-japonica hybrid seeds and the japonica-biased type indica-japonica hybrid seeds to obtain hybrid F2 generation seeds; stripping rice from the hybrid F2 generation seeds, weighing and selecting to obtain diploid small long-grain rice seeds; doubling doubling materials of the diploid small long rice seeds to obtain tetraploid rice materials; and breeding the tetraploid rice materials to obtain tetraploid rice seeds. Compared with traditional large-grain tetraploid rice, the grain number is increased by 40-60%, the effective grain number per unit area is increased by33.3-50.0%, the yield per unit area is increased by 20.0-25.0%, and the character can be stably inherited.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, in particular to a tetraploid rice breeding method. Background technique [0002] Rice is native to tropical Asia and is one of the three major food crops in the world and the most important food crop in my country. In my country, rice includes two subspecies of japonica and indica, both of which show obvious differences in morphology and distribution, and the F1 generation after hybridization often shows different degrees of pollen sterility. [0003] At present, rice breeding in the world includes conventional breeding (systematic selective breeding), cross breeding and molecular breeding. Plants in nature originate from diploids; however, polyploidization is an important way of plant evolution in nature. Rice is mostly diploid, and several tetraploids have been mutated in wild rice. At present, there are many tetraploid rice breeding units in the world. The main materials for doubling a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 曹国长舒园园王群
Owner WUHAN POLYPLOID BIOTECH CO LTD
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