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Centrifugal enrichment micro-fluidic chip for low-concentration liquid sample

A microfluidic chip and liquid sample technology, applied in the field of microfluidics, can solve the problems of small sample volume, low centrifugation efficiency, and low microbial concentration, and achieve the effect of simple operation, difficult diffusion and movement, and high efficiency

Active Publication Date: 2020-12-15
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Effectively solve the problem of low centrifugation efficiency caused by small sample volume and low microbial concentration in the sample in clinical testing

Method used

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  • Centrifugal enrichment micro-fluidic chip for low-concentration liquid sample
  • Centrifugal enrichment micro-fluidic chip for low-concentration liquid sample
  • Centrifugal enrichment micro-fluidic chip for low-concentration liquid sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Preparation of microfluidic chip:

[0041] ①Using the SU-8 photolithography method to make a microfluidic chip template.

[0042] ② Add a certain volume of SU-8 photoresist dropwise at the position of the chip sample storage cavity, and apply ultraviolet light to cure it to form an expanded and raised sample storage cavity support.

[0043] ③ Pour PDMS onto the microfluidic chip template, and cure at 70 degrees Celsius for 2 hours.

[0044] ④ Remove the PDMS layer from the template and cut it, and bond it with the glass slide through an oxygen plasma cleaning machine to complete the preparation of the microfluidic chip.

[0045] figure 1 and figure 2 The design schematic diagram and physical map of the fabricated microfluidic chip are respectively.

[0046] The enrichment microchannel 1 , the sample storage chamber 2 and the through hole 3 form a connected structure, wherein the enrichment microchannel 1 communicates with the sample storage chamber 2 t...

Embodiment 2

[0047] Example 2 sample centrifugation enrichment:

[0048] Using the microfluidic chip made in Example 1 to carry out centrifugal enrichment of samples, the specific steps are:

[0049] ① Puncture the liquid sample into the sample storage chamber through a syringe;

[0050] ②The microfluidic chip is placed in a centrifuge, the tip of the enrichment microchannel is far away from the center of the centrifuge, and the through hole is close to the center of the circle;

[0051] ③ According to the difference in density between the centrifuged product and the liquid, select an appropriate centrifugation speed for 10 to 30 minutes, so that the centrifuged product is enriched and fixed at the tip of the enrichment channel triangle.

[0052] Such as image 3 Shown is the image of the sample on the enrichment chip observed on the microscope, the centrifuged product is enriched and fixed at the tip of the enrichment channel triangle

Embodiment 3

[0053] Microbiological detection and microbial drug resistance evaluation of embodiment 3 clinical urine sample:

[0054] ①Take 100 microliters of urine samples and mix them with 1 milliliter of 2X broth medium, and add 32 microliters of 1 mg / mL ampicillin solution. The following steps are the same as those in Example 2 for centrifugal enrichment.

[0055] ② Observe the chip under a microscope to determine whether the sample is infected with bacterial microorganisms.

[0056] ③ Place the centrifuged chip in a 37°C incubator for static culture, observe the growth and replication of bacteria in the antibiotic culture environment every half hour, and evaluate antibiotic resistance, such as Figure 4 is the image observed every half hour, Figure 4 A, 4B, 4C, 4D are respectively the bacterial growth observed in 0 hour, 0.5 hour, 1 hour, and 1.5 hour.

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Abstract

The invention provides a centrifugal enrichment micro-fluidic chip for a low-concentration liquid sample, the micro-fluidic chip comprises a sample storage cavity and a sample enrichment micro-channel, the size of the enrichment micro-channel is close to the field of view of a microscope, the sample storage cavity is communicated with the enrichment micro-channel, During centrifugation, the distance between the enrichment micro-channel and the circle center is larger than the distance between the sample storage cavity and the circle center, so that components in the liquid sample are arrangedand enriched in the enrichment micro-channel according to the density.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a centrifugal enrichment microfluidic chip, method and application for low-concentration liquid samples. Background technique [0002] Centrifugation technology is widely used in the pre-enrichment treatment of various physical and chemical samples, such as the enrichment of cells and plankton in environmental and clinical water samples. Compared with filtration enrichment, centrifugation relies on the density difference between the target isolate and the liquid dispersed phase, so it avoids the loss of adsorption caused by contact with the filter membrane, and is convenient and simple to operate, and the cost of equipment is low. However, due to the poor centrifugation effect of existing centrifugation methods for low-concentration samples and small-volume samples, this is mainly due to the fact that traditional centrifuge tubes cannot achieve microliter-level centrifugati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L2200/12
Inventor 马波徐腾籍月彤徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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