Extraction and inclusion of hypoglycemic polysaccharides and polypeptides in inonotus obliquus and application of product in biscuits

A chaga and blood sugar-lowering technology, which is applied in the field of deep processing of miscellaneous grains, can solve the problems of loss of effect and instability, and achieve the effect of increasing lipid-lowering effect, raw material safety, and improving the function of pancreatic beta cells

Inactive Publication Date: 2020-12-15
吉林省桦树茸国际健康产业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the water extract of Chaga and the decomposed Chaga polypeptide are unstable and easily damaged by oxidation and lose their original effect. Therefore, it is necessary to study a method to protect it and ensure its antioxidant effect able to play effectively

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The extraction and inclusion of hypoglycemic polysaccharides and polypeptides in chaga include the following steps:

[0036] Step 1: crush Chaga and pass through a 100-mesh sieve, then reflux twice in a water bath at 70°C with an ethanol solution with a volume fraction of 85%, for 3 hours each time;

[0037] Step 2: Refrigerate and centrifuge at 3000 r / min for 20 min, remove the supernatant, and dry the pellet with hot air at 60°C for 12 hours to obtain pretreated chaga powder;

[0038] Step 3: Take the pretreated chaga powder and use microwave-assisted hot water to extract, the extraction temperature is 95°C, and the extraction time is 3 hours;

[0039] The fourth step: filter the extract, add water to the residue and extract again, extract twice in total, combine the filtrate, and filter to remove the residue;

[0040] Step 5: Add 95% ethanol to the filtrate for alcohol precipitation, centrifuge, and collect the precipitate;

[0041] Step 6: Freeze-dry the precipita...

Embodiment 2

[0054] The extraction and inclusion of hypoglycemic polysaccharides and polypeptides in chaga, including the following steps,

[0055] Step 1: crush Chaga and pass through a 100-mesh sieve, then reflux twice in a water bath at 70°C with an ethanol solution with a volume fraction of 85%, for 3 hours each time;

[0056] Step 2: Refrigerate and centrifuge at 3000 r / min for 20 min, remove the supernatant, and dry the pellet with hot air at 60°C for 12 hours to obtain pretreated chaga powder;

[0057] Step 3: Take the pretreated chaga powder and use microwave-assisted hot water to extract, the extraction temperature is 95°C, and the extraction time is 3 hours;

[0058] Step 4: filter the extract, add water to the residue to extract again, extract 3 times in total, combine the filtrate, and filter to remove the residue;

[0059] Step 5: Add 95% ethanol to the filtrate for alcohol precipitation, centrifuge, and collect the precipitate;

[0060] Step 6: Freeze-dry the precipitate to...

Embodiment 3

[0073] Step 1: crush Chaga and pass through a 100-mesh sieve, then reflux twice in a water bath at 70°C with an ethanol solution with a volume fraction of 85%, for 3 hours each time;

[0074] Step 2: Refrigerate and centrifuge at 3000 r / min for 20 min, remove the supernatant, and dry the pellet with hot air at 60°C for 12 hours to obtain pretreated chaga powder;

[0075] Step 3: Take the pretreated chaga powder and use microwave-assisted hot water to extract, the extraction temperature is 95°C, and the extraction time is 3 hours;

[0076] Step 4: filter the extract, add water to the residue to extract again, extract 3 times in total, combine the filtrate, and filter to remove the residue;

[0077] Step 5: Add 95% ethanol to the filtrate for alcohol precipitation, centrifuge, and collect the precipitate;

[0078] Step 6: Freeze-dry the precipitate to obtain chaga hypoglycemic polysaccharide;

[0079] Step 7: Take the pretreated chaga powder, add 16 times deionized water, stir...

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Abstract

The invention provides extraction and inclusion of hypoglycemic polysaccharides and polypeptides in inonotus obliquus and application of product in biscuits. The method comprises the following steps:pretreating inonotus obliquus to obtain pretreated inonotus obliquus powder; taking the pretreated inonotus obliquus powder, and performing extracting with microwave-assisted hot water; performing filtering to remove residues; adding ethanol into the filtrate for alcohol precipitation, performing centrifuging, and collecting precipitate; freeze-drying the precipitate to obtain the inonotus obliquus hypoglycemic polysaccharide; preparing inonotus obliquus polypeptide; dissolving insoluble dietary fibers in an acid solution, adding inonotus obliquus hypoglycemic polysaccharide, water-soluble dietary fibers and inonotus obliquus polypeptide, performing stirring, uniformly performing mixing, adding alkali liquor, performing neutralizing, drying and crushing to obtain the inonotus obliquus hypoglycemic component clathrate compound. The clathrate compound of the hypoglycemic component in the inonotus obliquus is applied to the biscuit to obtain the hypoglycemic biscuit. The biscuit capable of reducing blood sugar has a good antioxidant effect, becomes snacks in daily life, enriches life, and can meet all characteristics of leisure foods in modern society.

Description

technical field [0001] The invention relates to the field of deep processing of miscellaneous grains, in particular to the extraction and inclusion of hypoglycemic polysaccharides and polypeptides in chaga and their application in biscuits. Background technique [0002] Chaga velvet, also known as Inonotus obliquus, is a fungal phylum, Basidiomycotina, Laminomycetes, Phytomycetes, Polyporaceae, and Polyporus genus, and it mostly grows in cold regions. As early as the 16th century, Russia had used Inonotus obliquus, which had not undergone any pharmacological tests, as folk medicine to treat certain diseases, such as hyperlipidemia and diabetes. The appearance of the fruiting body of Inonotus obliquus is ellipsoidal, tumor-like, dark brown in color, within the range of 25-40 cm in diameter, with deep grooves on the outer surface and high hardness. The fungal flesh is corky with ring-shaped lines , Inonotus obliquus spores are smooth oval with bristles on the surface. Inonot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P21/06C07K1/34C07K1/14A21D13/80A21D2/36A21D2/26A21D2/18A21D2/02
CPCC08B37/0003A21D2/181A21D2/36A21D2/362A21D2/364A21D2/366A21D2/02A21D2/268A21D13/80C12P21/06C07K1/145C07K1/34
Inventor 姜镪徐建保
Owner 吉林省桦树茸国际健康产业集团有限公司
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