A kind of preparation method of nadh and ethanol biosensor chip
A biosensing and chip technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as research blanks, achieve the effect of wide linear range, simple preparation method, and uniform distribution
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Embodiment 1
[0031] An ethanol biosensing chip, the preparation process is as follows:
[0032] 1) Prepare 0.001M K 3 [Fe(CN) 6 ] and 0.001M NiCl 2 ·6H 2 O solution, adjust the pH of the solution to 6 with hydrochloric acid, respectively, to obtain a hydrochloric acid solution. The nickel ferricyanide precursor solution was synthesized by micro-speed chemical synthesis method: 90 ml of two hydrochloric acid solutions were taken out and fixed on a syringe pump, the synthesis temperature was 10 °C, and the injection speed was 100 µL / min. The low injection rate used in this example can reduce the crystallization rate of the crystals, resulting in more regular particles. After injection, stir for 15min, and then add 90mL NiCl dropwise at a rate of 100µL / min 2 ·6H 2 O hydrochloric acid solution. After the dropping is completed, the centrifugation rate of the mixed solution is 5000 r / min, and the centrifugation time is 10 min. Then use deionized water to centrifuge and clean for 2 times....
Embodiment 2
[0038] An ethanol biosensing chip, the preparation process is as follows:
[0039] 1) Prepare 0.01M K 4 [Fe(CN) 6 ] and 0.01M NiSO 4 The pH of the solution was adjusted to 4 with sulfuric acid, respectively. The nickel ferricyanide precursor solution was synthesized by micro-speed chemical synthesis method: 90ml of two acid solutions were taken out and fixed on a syringe pump, the synthesis temperature was 20°C, and the injection speed was 200µL / min. After injection, stir for 20min, and then add 70mL NiSO dropwise at a rate of 200µL / min 4 Sulfuric acid solution. After the dropping is completed, the centrifugation rate of the mixed solution is 6000r / min, the centrifugation time is 10min, and then centrifuged with deionized water for 3 times, the centrifugal speed is 6000r / min, 10min each time, and the obtained solid is redispersed in 70mL deionized water. , to obtain a nickel ferricyanide suspension.
[0040] 2) Configure 2mM HAuCl 4 solution and 10mM sodium citrate solu...
Embodiment 3
[0045] An ethanol biosensing chip, the preparation process is as follows:
[0046] 1) Prepare 0.05M K 4 [Fe(CN) 6 ] and 0.05M Ni (NO 3 ) 2 The pH of the solution was adjusted to 3 with nitric acid, respectively. The nickel ferricyanide precursor solution was synthesized by micro-speed chemical synthesis method: 90 ml of the two solutions were taken out and fixed on a syringe pump, the synthesis temperature was 40 °C, and the injection speed was 500 µL / min. After the injection was completed, stir for 40 min, and then add 50 mL of Ni(NO) dropwise at a rate of 500 µL / min. 3 ) 2 Nitric acid solution. Centrifuge after the dropwise addition, the centrifugation rate of the mixture is 8000r / min, the centrifugation time is 6min, and then centrifuged with deionized water for 4 times, the centrifugation rate is 6000r / min, 6min each time. In ionized water, a nickel ferricyanide suspension was obtained.
[0047] 2) Prepare 2mM HAuCl 4 solution and 10mM glucose solution, take 20mL ...
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