Hemerocallis fulva tissue culture seedling domestication method

Abstract

The invention belongs to the field of plant tissue culture, and particularly discloses a hemerocallis fulva tissue culture seedling domestication method. The method comprises the steps of rooting culture, seedling culture substrate selection, seedling body disinfection, seedling culture management, field transplanting and the like, wherein a 1 / 2MS+0.5 mg / LNAA+0.3 mg / LMET culture medium is creatively used during rooting culture, the rooting rate can be obviously improved, although the length of new roots is slightly reduced, the roots are relatively thick and strong and mostly grow in a radialshape; and the quality of tissue culture seedlings can be obviously improved, so that leaves are thick in texture, dark green in color and robust. NAA and MET with proper mass concentration are used together to facilitate rooting of rootless tissue culture seedlings of hemerocallis fulva, and the transplanting survival rate of rooted test-tube plantlets can reach up to 100% in cooperation with a seedling culture substrate and a field management method used in the method.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for domesticating a tissue culture seedling of Hemerocallis. Background technique [0002] Plant tissue culture is a high-tech method that uses the principle of asexual reproduction to make plant tissues rapidly develop into new plants through proliferation and differentiation under artificially controlled conditions. In recent years, with the rapid development of science and technology, most plant tissue culture technology has entered the stage of production and application. Nowadays, the tissue culture technology of Hemerocallis spp. has reached a mature stage, and many scholars have carried out comprehensive research on the whole tissue culture process from initiation culture, proliferation culture, rooting culture, domestication and transplantation, but the focus is on the whole process of tissue culture. In the research of initiation and proliferation culture, t...

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Problems solved by technology

Today, the tissue culture technology of Hemerocallis spp. has reached a mature stage. Many scholars have conducted comprehensive research on the entire tissue culture process from initiation culture, proliferation culture, rooting culture, domestication and transplanting, but the focus is on In the study of initiation and proliferation culture, there is a lack of systematic research on the links of rooting, domestication and transplanting, and the research on rooting mostly uses the medium added with different concentrations of NAA, IBA, and IAA3 growth hormones for rooting culture, while There is no report on the rooting of Hemerocallis tissue culture seedlings by using paclobutrazol (MET), but there are many reports on the research on tissue culture seedlings of other plants

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