Detection method of a traditional Chinese medicine composition for nourishing yin and clearing away lung-heat

Abstract

The invention relates to a detection method of a traditional Chinese medicine composition for nourishing yin and clearing away lung-heat. The method comprises the following steps: preparation of a reference substance solution: taking paeoniflorin, harpagoside and paeonol as reference substances, and dissolving with a solvent; preparing a test solution, namely extracting a to-be-detected sample byusing an extraction solvent, filtering an extracting solution, and taking subsequent filtrate as the test solution; injecting the reference substance solution and the test solution into a liquid chromatograph for determination, wherein a mobile phase A of the liquid chromatograph is water, a 0.005 mol / L-0. 04mol / L sodium dihydrogen phosphate aqueous solution or a 0.005 mol / L-0. 04mol / L potassium dihydrogen phosphate aqueous solution, and a mobile phase B of the liquid chromatograph is acetonitrile. The method provided by the invention realizes simultaneous determination of multiple componentsin the yin-nourishing lung-heat-clearing traditional Chinese medicine composition, has strong specificity and good separation effect, has accuracy, reproducibility and stability meeting scientific research and production requirements, and can effectively ensure the stability and controllability of product quality.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a detection method of a traditional Chinese medicine composition for nourishing yin and clearing lung. Background technique [0002] Yangyin Qingfei composition is composed of rehmannia glutinosa, scrophulariae, white peony root, Moutan cortex, Ophiopogon japonicus, Chuan Fritillaria, mint, and licorice. Lung mixture. [0003] Yangyin Qingfei ointment has the effect of nourishing yin and moistening dryness, clearing lung and relieving throat. It is mainly used to treat yin deficiency and dryness of the lung, dry throat, dry cough with little phlegm or bloody sputum. It is composed of rehmannia glutinosa, scrophulariaceae, white peony root, peony bark, Radix Ophiopogon japonicus, Chuan Fritillaria, mint, and licorice. At present, there are 20 manufacturers of Yangyin Qingfei Ointment. This variety is included in the 2015 edition of "Chinese Pharmacopoeia". The property is too low, it is...

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Problems solved by technology

The standard is recorded in the 20th Volume WS3-B-3896-98 of the "Standards issued by the Ministry of Health of the People's Republic of China" for the preparation of traditional Chinese medicines. There are only traits, identification, inspection items and microbial limit inspection items. The monitoring method is relatively low, and the existing literature content determination method only has Fang Wenqing's "TLC method and HPLC method to the quality control of Yangyin Qingfei mixture", and only single component catalpol is used for content determination in the paper, while the literature "The Effects of RehmanniaGlutinosa polysaccharide on immune function of mice pointed out that catalpol is an iridoid glycoside compound, its chemical properties are active, easy to be hydrolyzed and oxidized, resulting in a large decrease in catalpol and poor stability, so the other active ingredients in the other party The determination is more scientific and reasonable

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