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Rabbit normal-temperature antioxidant preservation diluent, preparation method and use method thereof

A diluent and anti-oxidant technology, applied in the field of animal reproduction, can solve the problems of increased deformity rate and decreased semen vitality of rabbits, and achieve the effects of reducing oxidative damage, reducing sperm oxidative stress and prolonging storage time.

Inactive Publication Date: 2020-12-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a diluent for anti-oxidation preservation of rabbits at room temperature and its preparation method, so as to solve the technical problems of reduced vitality and increased deformity rate of rabbit semen during preservation

Method used

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  • Rabbit normal-temperature antioxidant preservation diluent, preparation method and use method thereof
  • Rabbit normal-temperature antioxidant preservation diluent, preparation method and use method thereof
  • Rabbit normal-temperature antioxidant preservation diluent, preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A diluent for anti-oxidation preservation of rabbits at room temperature, the ingredients and content in 100mL of the diluent are 1.3g of glucose, 1.3g of fructose, 1.7g of tris, 1.6g of citric acid, and 1.6g of ethylenediaminetetraacetic acid Sodium 0.17g, bovine serum albumin 0.75g, dihydromyricetin 0.010g, penicillin 100,000 IU, streptomycin 100,000 IU, add double distilled water to make up to 100mL.

[0023] The preparation steps of the diluent are: (1) Weighing 1.3g of glucose, 1.3g of fructose, 1.7g of tris, 1.6g of citric acid, 0.17g of disodium edetate, and 0.17g of bovine serum white Protein 0.75g, penicillin 100,000 IU, streptomycin 100,000 IU, add double-distilled water to 100mL, mix well, filter with 0.22μm filter; (2) add 0.010g of dihydromyricetin to step (1) In the obtained solution, a diluent can be obtained, and the pH is 6.8.

[0024] Detect the collected male rabbit semen, select the semen with an activity greater than 0.8 to mix, pre-warm the semen ...

Embodiment 2

[0051] A diluent for anti-oxidation preservation of rabbits at room temperature, the ingredients and content in 100mL of the diluent are 1.9g of glucose, 1.9g of fructose, 1.7g of tris, 1.6g of citric acid, and 1.6g of ethylenediaminetetraacetic acid Sodium 0.17g, bovine serum albumin 1.0g, dihydromyricetin 0.005g, penicillin 100,000 IU, streptomycin 100,000 IU.

[0052] The preparation steps of 100mL of the diluted solution are: (1) Weighing 1.9g of glucose, 1.9g of fructose, 1.7g of tris, 1.6g of citric acid, 0.17g of disodium edetate, bovine serum Albumin 1.0g, penicillin 100,000 IU, streptomycin 100,000 IU, add double distilled water to 100mL, shake well, filter with 0.22μm filter; (2) add 0.005g dihydromyricetin to In the solution obtained in step (1), the diluted solution can be obtained, and the pH is 6.8.

[0053] Detect the collected male rabbit semen, select the semen with an activity greater than 0.8 to mix, pre-warm the semen at 37°C to make the temperature of the...

Embodiment 3

[0067] A diluent for anti-oxidation preservation of rabbit semen at room temperature, the components of the composition and the content in 100 mL are: 1.0 g of glucose, 1.0 g of fructose, 1.7 g of tris, 0.9 g of citric acid, and ethylenediamine Disodium tetraacetate 0.17g, bovine serum albumin 0.25g, dihydromyricetin 0.005g, penicillin 100,000 IU, streptomycin 100,000 IU; the pH of the diluent is 6.8; the normal temperature is 17°C.

[0068] The preparation steps of 100mL of the diluted solution are: (1) Weighing 1.0g of glucose, 1.0g of fructose, 1.7g of Tris, 0.9g of citric acid, 0.17g of disodium edetate, bovine serum Albumin 0.25g, penicillin 100,000 IU, streptomycin 100,000 IU, add double distilled water to 100mL, shake well, filter with 0.22μm filter; (2) add 0.005g dihydromyricetin to In the solution obtained in step (1), the diluted solution can be obtained, and the pH is 6.8.

[0069] Detect the collected male rabbit semen, select the semen with an activity greater t...

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Abstract

The invention relates to a rabbit normal-temperature antioxidant preservation diluent, a preparation method and a use method thereof, and belongs to the technical field of animal reproduction. 100 mLof the composition comprises the following components: 1.0-1.9 g of glucose, 1.0-1.9 g of fructose, 1.7 g of tris(hydroxymethyl) aminomethane, 0.9-1.8 g of citric acid, 0.17 g of disodium ethylene diamine tetraacetate, 0.25-1.5 g of bovine serum albumin, 0.005-0.015 g of dihydromyricetin, 100000 IU of penicillin, and 100000 IU of streptomycin, wherein the pH value of the diluent is 6.8-7.0, and the normal temperature is 17 DEG C. According to the invention, the preservation diluent can solve the problem of oxidative damage in a rabbit semen preservation process, prolong the normal-temperaturepreservation time of semen, facilitate long-distance transportation, enable the rabbit artificial insemination technology not to be limited by factors such as time, space and the physiological cycle of breeding rabbits any more, and remarkably improve the rabbit artificial insemination efficiency.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, in particular to a diluent for anti-oxidation preservation of rabbit semen at room temperature, a preparation method and a use method thereof, which are used for long-term preservation of rabbit semen at room temperature and artificial insemination. [0002] technical background [0003] Rabbit breeding is a characteristic industry in my country's rural economic development at present. It requires less start-up capital, has a fast operation cycle, and has high economic benefits. It is an ideal industry for getting rid of poverty and becoming rich. In recent years, with the development of the rabbit breeding industry, the intensive and large-scale level of rabbit farms has been continuously improved, and the breeding benefits have been continuously improved, and some achievements have been made. However, many problems have been exposed during the rapid development of the industry. Among ...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/0215A01N1/0226C12N5/061
Inventor 剧世强蔡云栋范志宇芮荣贺依静
Owner NANJING AGRICULTURAL UNIVERSITY