Variable region sequence of specific anti-pyraclostrobin antibody and anti-pyraclostrobin recombinant overall-length antibody

Abstract

The invention discloses a variable region sequence of a specific anti-pyraclostrobin antibody. The amino acid sequence of a heavy chain variable region encoding gene is disclosed by SEQ ID NO: 2. Theinvention also discloses an anti-pyraclostrobin recombinant overall-length antibody, which comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein the amino acid sequence of the heavy chain variable region encoding gene is disclosed by SEQ ID NO: 2. The invention also discloses an antibody expression plasmid.A sequence gene obtained by the invention is independently connected to expression carriers containing a heavy chain constant region gene and a light chain constant region gene, mammal cell expressionof a double-plasmid system is adopted to obtain the recombinant overall-length antibody, the recombinant overall-length antibody has consistent identification activity with a murine parent antibody,the anti-pyraclostrobin antibody can be stably produced on a large scale, and a reliable core reagent is provided for various immunoassay methods for detecting pyraclostrobi residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation and application of a variable region sequence of a specific anti-pyraclostrobin antibody and its recombinant full-length antibody. Background technique [0002] Pyraclostrobin is a new type of strobilurin fungicide developed by BASF (BASF) in 1993. It prevents electron transfer between cytochrome B and C1, inhibits mitochondrial respiration and inhibits The production of ATP eventually leads to the death of pathogenic fungi that cannot carry out normal physiological metabolism. As an environmentally friendly fungicide, pyraclostrobin is less harmful to non-target organisms, but it has been reported to have certain mitochondrial toxicity and neurotoxicity to silkworms and zebrafish. Therefore, pyraclostrobin should be controlled in agricultural production. The use of bacteriocin. According to the latest national standard (GB 2763-2019), th...

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Problems solved by technology

[0005] However, hybridoma cells have a complex genetic background and gene deletions/mutations that occur during long-term passage and thawing and freezing, which can easi...

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