A strain of Bacillus licheniformis Umami No. 1 with the ability to inhibit the formation of earthy smell substances in aquatic products and its application

A technology of bacillus licheniformis and antibacterial effect, applied in the direction of application, microbe-based methods, chemicals for biological control, etc., can solve the problems of toxic side effects, loss, and weight loss of aquatic animals, etc., to ensure that the breeding Safety and environmental friendliness, effect of changing bacterial flora structure, growth inhibition

Active Publication Date: 2022-04-19
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the source of GSM and 2-MIB is gradually clarified, but the control effect of soil odor substances is still affected by many factors such as cultured species, culture environment, and the length of enrichment time in aquatic animals.
Some aquatic products usually choose to be kept in clean water before they go on the market to reduce the earthy smell substances in the body, but the suspension of feeding and transshipment will lead to weight loss and loss of aquatic animals
[0006] In addition, there is also a method of using dissolved ozone to oxidize GSM and 2-MIB to remove the smell of soil in the water body. Although this method has been widely used in the treatment of GSM and 2-MIB in municipal drinking water, because this method is harmful to ozone There are certain requirements for the concentration. If a higher dose of ozone is used, it may cause toxic and side effects to the aquaculture objects. Therefore, the application of this method in the aquaculture process is still limited.

Method used

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  • A strain of Bacillus licheniformis Umami No. 1 with the ability to inhibit the formation of earthy smell substances in aquatic products and its application
  • A strain of Bacillus licheniformis Umami No. 1 with the ability to inhibit the formation of earthy smell substances in aquatic products and its application
  • A strain of Bacillus licheniformis Umami No. 1 with the ability to inhibit the formation of earthy smell substances in aquatic products and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Isolation and screening of strains

[0031] 1. Strain isolation

[0032] Collect the surface attachments of biological fillers from the recirculating aquaculture system, dissolve the samples with 0.85% sterile saline, serially dilute 6 times by 10 times, and spread them on soybean casein agar medium (TSA), numbering, each sample Do 3 repetitions. Cultivate at 37°C for 24 hours, select colonies with different shapes, fast growth and large diameter, and inoculate them on ordinary broth plates for isolation and purification.

[0033] 2. Strain screening

[0034] The antibacterial performance of the isolated strains was measured by the Oxford plate antibacterial method. After a large number of sorting, four strains were obtained that had inhibitory effects on Streptomyces thermocarboxydus and Streptomyces cyaneofuscatus, which produced odorous substances. Alternative strains, the specific operations are as follows:

[0035] Inoculate Streptomyces carbomonoxide thermo...

Embodiment 2

[0065] Embodiment 2 broth culture experiment

[0066] Experimental settings: normal saline control group (A), single-bacteria culture group of No. The culture group (D) uses TSB medium at 37° C. with a rotational speed of 110 rpm. Sampling at 0, 6, 12, 24, 48, 72, 96, 120, 144, 168, 192, 216 and 240 hours respectively, counting the colonies of Umami strain 1 and Streptomyces thermophilic carbon monoxide by plate method .

[0067] The result is as image 3 As shown, after 24h of culturing, the concentration of Streptomyces thermophilic carbon monoxide in group C increased from 10 2 CFU / mL increased to 10 5 CFU / mL, and the concentration can be maintained for 120h, and then the concentration gradually decreased. In group D, the concentration of Streptomyces carbon monoxide reached 10 after 48 hours of culture. 6 CFU / mL, the concentration began to decrease significantly after that, and dropped to 10CFU / mL at 72h, and then remained in the low concentration range.

Embodiment 3

[0068] Embodiment 3 hemolysis experiment

[0069] Dilute 5 μl to a concentration of 10 8 CFU / mL culture solution of Xianwei bacteria No. 1 strain was inoculated on sheep blood plate medium, and incubated at 37°C for 24 hours to observe whether there was hemolysis. The judgment method is as follows: α-hemolysis, a 1-2 mm grass green hemolysis ring appears in the culture medium around the colony; β-hemolysis, a wide, well-defined, completely transparent hemolysis ring is formed around the colony, with a diameter of about 2-4 mm; if there is no hemolysis ring Appears, there is no hemolysis phenomenon. The result is as Figure 4 As shown, Streptococcus agalactiae as a positive control had a hemolytic ring, and the Umami strain No. 1 did not have a hemolytic ring and no hemolytic phenomenon.

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Abstract

The invention discloses a strain of bacillus licheniformis umami bacteria No. 1 which can inhibit the formation of earthy smell substances in aquatic products and its application. The strain is preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and its preservation number is CGMCC NO.20406. The No. 1 bacterial strain of Bacillus licheniformis umami bacteria provided by the invention has the ability to inhibit the activity of Streptomyces thermophilic carbon monoxide and brown fungi. production, and at the same time can ensure the safety and environmental friendliness of breeding; by adding the No. The effective concentration of the strain No. 1 was 10 5 CFU / mL.

Description

technical field [0001] The invention relates to a Bacillus sp. umami bacteria No. 1 strain with the ability to inhibit the formation of fishy smell substances in aquatic products and an application thereof, which belongs to the field of aquaculture environment microorganisms. Background technique [0002] Earthy smell has always been the main factor affecting the quality and consumption behavior of aquatic products. The existence of earthy smell in the aquaculture system will cause the fish to have an earthy smell, thus losing its own fishy taste. This problem has plagued the aquaculture industry for a long time, and has caused huge economic losses to domestic and foreign farmers and aquatic product processing enterprises. In recent years, due to the problem of earthy smell of aquatic products, the situation that the products cannot be listed has occurred from time to time. For example, the prawns exported from Ecuador to the United States were banned from the market due to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01K61/00A01N63/22A01P1/00C12R1/10
CPCC12N1/20A01K61/00A01N63/22C12R2001/10C12N1/205Y02A40/81
Inventor 刘利平李慷
Owner SHANGHAI OCEAN UNIV
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