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Detection method for rapidly identifying quality of cold-pressed peanut oil

A detection method, peanut oil technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of incapable quantitative analysis of aldehydes and cumbersome processing process, and achieve the effects of shortening derivation time, good anti-interference performance and improving sensitivity

Pending Publication Date: 2020-12-22
中科检测技术服务(广州)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not only cumbersome in the pretreatment process, but also cannot quantitatively analyze the aldehydes in the oil

Method used

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  • Detection method for rapidly identifying quality of cold-pressed peanut oil
  • Detection method for rapidly identifying quality of cold-pressed peanut oil
  • Detection method for rapidly identifying quality of cold-pressed peanut oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In the present invention, aluminum oxide, nitrosobenzene and aniline are selected as catalysts for the formation of hydrazone structure products. Include the following steps:

[0036] 1. Instruments, reagents and materials

[0037] Instruments: LC-20AT high performance liquid chromatography, RF-20A fluorescence detector, electronic balance (Shimadzu, Japan); constant temperature water bath (Guohua Electric Co., Ltd.); pH acidity meter (Mettler-Toledo); Pipette gun (Eppendorf); Reversed-phase C18 chromatographic column 5 μm, 4.6*250mm (GL Science) Reagent:

[0038] Table 1 Standards and Reagents

[0039]

[0040]

[0041] The water used was ultrapure water.

[0042] 2. Solution preparation and derivation

[0043] DNSH (dansyl hydrazide) solution preparation: weigh 25 mg of dansyl hydrazide and add it to a 50 mL brown volumetric flask, add an appropriate amount of acetic acid / acetonitrile mixed solvent (wherein acetic acid accounts for 3% of the total solvent vo...

Embodiment 2

[0054] 1. Solution preparation and derivation

[0055] Three kinds of furfural concentrations in embodiment 1 are all the furfural mixed solutions of 1000mg / L with methanol dilution to obtain the secondary mixed standard stock solution of 100mg / L, then diluted to three kinds of furfural concentrations are 1mg / L to obtain the mixed furfural standard solution. Accurately pipette 0.1mL, 0.2mL, 0.5mL, 1mL, 2mL and 5mL of mixed furfural standard solutions (all three furfural concentrations are 1mg / L) into 6 centrifuge tubes, and then add 1mL of 500mg / L of dansyl hydrazide solution, and then use acetic acid / acetonitrile mixed solvent (wherein acetic acid accounts for 3% of the total solvent volume fraction) to make up to 10 mL; finally derivatize at 25°C for 20 min.

[0056] 2. Detection conditions

[0057] The derivatized solution was detected by HPLC-FLD, the instrument model was Shimadzu LC-20AT, the chromatographic column was GL Science reversed-phase C18 chromatographic colu...

Embodiment 3

[0066] Self-made cold-pressed peanut oil and hot-pressed peanut oil are qualitatively and quantitatively tested, including the following steps:

[0067] (1) Hot-pressed peanut oil is to adjust the moisture content of the screened peanuts to 10%, roast the peeled peanuts at 200°C for 30 minutes, squeeze the oil with an oil press, and then centrifuge and filter the crude oil; cold-pressed peanut oil is The peeled peanuts are directly squeezed out with an oil press, and then the crude oil is centrifuged and filtered.

[0068] (2) Weigh 10 g of homemade hot-pressed and cold-pressed peanut oil samples and place them in two 50-mL centrifuge tubes, and add 1 mL of dansylhydrazide solution with a concentration of 500 mg / L and 1 mL of dansylhydrazine solution with a concentration of 1000 mg / L to each of them. For the aniline solution, dilute to 50ml with methanol, vortex for 30s, mix well, and derivatize in a water bath at 25°C for 20min. Extract the derivatized peanut oil three times...

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Abstract

The invention discloses a detection method for rapidly detecting furfural substances in peanut oil. According to the method, under the action of a catalyst aniline, furfural substances in the peanut oil and a fluorescent reagent dansyl hydrazide are subjected to hydrazone reaction to generate furfural derivatives, and the derivatives can be detected by HPLC-FLD, so that the quality of the cold-pressed peanut oil is qualitatively and quantitatively identified. According to the method, furfural substances and dansyl hydrazide can quickly react, the derivation reaction time is short, the detection sensitivity is high, the detection limit is low, accuracy and reliability are achieved, and the method can be applied to identification of the quality of the cold-pressed peanut oil.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a detection method for quickly identifying the quality of cold-pressed peanut oil. Background technique [0002] Peanut oil is one of the most used vegetable oils in daily life. Cold pressing and hot pressing are the main extraction methods of peanut oil at present. Cold-pressed peanut oil is made by drying peanuts at low temperature, removing the red coat, and directly adopting physical and mechanical extrusion methods to obtain cold-pressed oil. The temperature of cold pressing is lower than 65°C to room temperature, which is much lower than that of hot pressing oil. In this process, peanut oil nutrition and bioactive substances have been completely preserved. However, the oil yield of cold-pressed oil is low, and the cost is higher compared with traditional hot-pressed oil. In order to pursue greater profits, some merchants will mix hot-pressed oil with finished co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/74
CPCG01N30/89G01N30/74
Inventor 张傲彭诗琪黄晓玲赵嘉辉孙家豪杜磊徐华超源嘉辉
Owner 中科检测技术服务(广州)股份有限公司