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Application of OsKEAP1 gene in regulation and control of salt stress resistance of rice

A technology of salt stress and genetics, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems that plants are rarely studied

Inactive Publication Date: 2020-12-25
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As an essential functional protein in the oxidative stress response pathway in animals, KEAP1 plays an important role in the regulation of oxidative stress, but it is rarely studied in plants

Method used

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  • Application of OsKEAP1 gene in regulation and control of salt stress resistance of rice
  • Application of OsKEAP1 gene in regulation and control of salt stress resistance of rice
  • Application of OsKEAP1 gene in regulation and control of salt stress resistance of rice

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Embodiment 1

[0023] 1. Identification and characterization of KEAP1 homologous genes in rice

[0024] The gene information of human KEAP1 and its encoded amino acid sequence were obtained from NCBI, and compared with the rice genome in the Gramene database using it as an index sequence, to obtain KEAP1 homologous genes in the rice genome. The structure of the rice KEAP1 gene and the characteristics of the encoded protein were analyzed in the KEGG database and the RAPDB database, and the amino acid conserved domain was analyzed in the Unipro database and the SMART database. The changes of their expression levels in different plant tissues and hormone treatments were analyzed in RiceXPro software.

[0025] The amino acid sequences of maize, human and 10 other related species were downloaded from the Gramene database and OrthoDB database, and the NJ method was used to construct the KEAP1 orthologous gene phylogenetic tree with the Bootstrp repeat number set of 1000 as the minimum evolution st...

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Abstract

The invention discloses an application of an OsKEAP1 gene in regulation and control of salt stress resistance of rice. The nucleotide sequence of the OsKEAP1 gene is shown as SEQ ID NO. 1. The salt stress resistance is mainly reflected in the content change of hydrogen peroxide, proline and malondialdehyde in the mutant before and after salt stress and the difference of expression levels of salt stress related genes after salt stress treatment. According to the application, the mutant with down-regulated OsKEAP1 gene expression level is obtained by using a CRISPR / Cas9 gene editing technology,and it is found that after OsKEAP1 mutation, the change of an anti-oxidative stress pathway in rice is caused, and then the change of salt resistance of rice is caused, so that a basis can be providedfor culturing rice varieties with excellent salt stress resistance.

Description

technical field [0001] The invention relates to the technical fields of plant genetic engineering and rice molecular breeding, and mainly relates to the application of the OsKEAP1 gene in regulating the resistance to salt stress of rice. Background technique [0002] Reactive oxygen species (ROS) is a general term for oxygen-containing molecules or ions with strong oxidative activity, and is a natural by-product of oxygen-related metabolism in animal and plant cells. The delicate balance that exists between the accumulation of ROS and the antioxidant systems that scavenge them is known as redox homeostasis. In higher plants, ROS are also important signaling molecules in salt- and drought-related stress. The imbalance between ROS accumulation and antioxidant scavenging system can cause oxidative stress (Oxidative stress, OS), which will lead to a series of stress responses such as the destruction of intracellular biological macromolecules and inflammatory infiltration in bio...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8218C12N15/8273
Inventor 舒庆尧刘燕华蒋萌
Owner ZHEJIANG UNIV
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