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Device and method for improving integrated partial denitrification-anaerobic ammonia oxidation coupling denitrification performance

A technology of anaerobic ammonium oxidation and denitrification, applied in anaerobic digestion treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of inability to remove nitrate, low yield, and weak environmental stress resistance And other issues

Pending Publication Date: 2020-12-29
BEIJING UNIV OF TECH
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Problems solved by technology

However, the PN / ANAMMOX process cannot remove nitrate contained in wastewater, resulting in relatively poor nitrogen removal efficiency (51-87%)
In addition, Anammox bacteria cells also have low yield (0.11gVSS / gNH4+-N), long generation cycle (up to 32d), strong dependence on substrate, weak environmental stress resistance and difficult gene amplification, which lead to time-consuming reaction start-up long feature

Method used

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  • Device and method for improving integrated partial denitrification-anaerobic ammonia oxidation coupling denitrification performance

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Embodiment 1

[0057] specific structure, see figure 1 .

[0058] (1) Components of Acinetobacter JQ1004 Medium

[0059] LB medium: NaCl 10.00g·L -1 , tryptone 10.00g·L -1 , Yeast extract 5.00g·L -1 ;

[0060] Denitrification medium (isolation and purification medium): (CH 2 COONa) 2 ·6H 2 O 4.136g·L -1 ,KNO 3 0.72g·L -1 ,0.565g·L -1 K 2 HPO 4 0.565g L-1, MgSO 4 ·7H 2 O 0.20g·L -1 , 2.50mL trace element, trace element solution: Na 2 EDTA 50.00g·L -1 ,ZnSO 4 ·7H 2 O 2.20g·L -1 ,CaCl 2 5.50g·L -1 , MnCl·4H 2 O5.06g·L -1 ,FeSO4 5.00g·L -1 ,CuSO 4 ·5H 2 O 1.57g L -1 ,CoCl 2 ·6H 2 O 1.60g·L -1 , both media were sterilized at 121°C for 15 min before use, and the pH was adjusted by Na 2 HPO 4 and NaH 2 PO 4 The buffer solution is adjusted between 7.0 and 7.3. The solid medium needs to add 1.5% to 2% agar.

[0061] (2) Culture of anammox bacteria

[0062]Artificially simulated domestic sewage is used to cultivate bacteria, and the water distribution component ...

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Abstract

The invention discloses a device and a method for improving integrated partial denitrification-anaerobic ammonia oxidation coupling denitrification performance, and belongs to the field of sewage treatment. Partial denitrification granular sludge is arranged at the bottommost part of the reactor, anaerobic ammonia oxidation granular sludge is arranged at the middle part of the reactor, and the anaerobic ammonia oxidation granular sludge in the reactor is uniformly mixed with acinetobacter JQ1004 embedded particles. The method comprises the following steps: carrying out enhanced starvation culture on acinetobacter JQ1004, so that the acinetobacter JQ1004 grows in a stable period and can secrete more acyl homoserine lactone (AHLs) signal molecules, and then putting cultured acinetobacter JQ1004 embedded particles in the stable period and anaerobic ammonia oxidation particle sludge into the same reaction device. The acinetobacter JQ1004 can consume an organic carbon source to reduce nitrate nitrogen under an anoxic condition, the influence of organic matters on anaerobic ammonia oxidation is reduced, and meanwhile, acyl homoserine lactone signal molecules generated by the acinetobacter JQ1004 can also improve the activity of anaerobic ammonia oxidizing bacteria.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to a sewage treatment device and its operation strategy based on partial denitrification sludge particles, entrapped Acinetobacter JQ1004 (No. . Background technique [0002] Anaerobic ammonium oxidation, the process of oxidizing ammonia into nitrogen by using nitrite as an electron acceptor, can reduce oxygen demand through anaerobic ammonium oxidation biological denitrification, and a part of ammonia can be oxidized without consuming oxygen under anoxic conditions. Therefore, it is widely regarded as a cost-effective wastewater denitrification process. In order to obtain nitrite as a substrate, ANAMMOX is usually combined with partial denitrification (PN), which can theoretically reduce aeration by 60%, reduce organic carbon by 100%, and reduce sludge production by 90% . The partial denitrification / anammox (PDA) process is considered as a promising method to implemen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34C02F101/16C02F101/30
CPCC02F3/2806C02F3/2846C02F3/286C02F3/34C02F2101/163C02F2101/30
Inventor 李军韩浩张晶张一魏鹏元周荣煊
Owner BEIJING UNIV OF TECH
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