Jujube witches broom phytoplasma effector gene Zaofeng8 and application thereof

Abstract

The invention discloses a jujube witches broom phytoplasma effector gene Zaofeng8 and application. The nucleotide sequence of the jujube witches broom phytoplasma effector gene Zaofeng8 is shown as SEQ ID NO.1, the gene has the functions of dwarfing plants, enabling the plants to form arbuscular phenotypes and enabling the plants to generate leaflet symptoms, and the Zaofeng8 is obtained through PCR technology cloning. The gene is transferred into Columbia type arabidopsis thaliana for functional verification by using an agrobacterium-mediated method, so that the infection mechanism of phytoplasma to hosts in the occurrence process of the jujube witches broom symptom can be clarified from a molecular mechanism, and the gene has a positive guiding effect on further prevention and treatmentof the jujube witches broom.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Zaofeng8, a phytoplasma effector gene of jujube madness, and its application. Background technique [0002] When plant pathogens infect host plants, they will activate the plant's own defense response, thereby inhibiting the infection of pathogens; however, pathogens will secrete effector factors to inhibit the host's defense response, so as to ensure that pathogens can better invade plants and obtain nutrients. In which way the effectors work together to destroy the plant's defense response and achieve the goal of successful pathogen infection needs to be continuously explored. At the molecular level, the research on pathogenic targets of pathogenic bacteria provides a theoretical basis for the breeding of disease-resistant varieties and the research and development of ecological pesticides, and effectively solves the food safety caused by the enhanced drug resistance of...

AI Technical Summary

Problems solved by technology

The abnormal developmental phenotypes of jujube trees infected with jujube disease, such as arbuscular branches, clusters of small leaves, plant dwarfing, deformed floral organs and flower leaves, are due to the infection of its pathogen, Candidatus phytoplasma ziziphi. A special kind of bacteria obligately parasitizes the sieve tube cells of the plant phloem, and the harsh growth conditions cannot be cultured in vitro, which brings great difficulties to the prevention and treatment of jujube madness

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