Full-automatic single cell capture chip based on digital microfluidic technology and application of full-automatic single cell capture chip

Abstract

The invention discloses a full-automatic selective single cell capture device based on a digital microfluidic technology and application of the full-automatic selective single cell capture device. Thefull-automatic selective single cell capture device comprises a digital microfluidic chip, an imaging system located above the digital microfluidic chip and a control circuit connected with the digital microfluidic chip and the imaging system. The single cell capture device can achieve full-automatic single cell selective capture, the capture success rate is 100%, the obtained single cells are classified and identified through morphology and molecular biology, high specificity and high sensitivity are achieved, and the single cell capture device is suitable for completing single cell separation and follow-up application of circulating tumor cells in blood.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, in particular to a fully automatic single particle / single cell capture chip based on digital microfluidics technology and its application. Background technique [0002] Cells are the basic unit of biological structure and function. Cell analysis can provide important theoretical basis for physiological processes such as metabolism and signal transduction. Most of the traditional cell analysis is based on population cells, and the analysis is the average result of a large number of cells, which is an average data of the population state. At present, more and more studies have shown that there is heterogeneity among individual cells. A group of cells that seem to be the same may have essential differences inside them, and there are differences in genome sequence, enzyme activity, protein expression, etc. difference. These differences are reflected in the heterogeneity of cell functi...

AI Technical Summary

Problems solved by technology

However, the limitations of conventional microfluidic chips are also very obvious. It requires the use of mechanical pumps and valves, which makes integration difficult, and it is difficult to achieve continuous multi-step processing of samples; it is difficult to accurately control the position of multiple reagents and reaction time; And it is impossible to achieve single-cell capture of a small number of rare cells, and it is even more powerless in the field of high-efficiency and high-throughput analysis of a large number of single cells

Images