A rapid screening method for anemone toxin-producing microorganisms

A screening method and microbial technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of heavy workload, long cycle, and high analysis cost, and achieve high accuracy, high specificity, and screening cycle short effect
CN112176034BActive Publication Date: 2022-07-15舟山出入境检验检疫局综合技术服务中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
舟山出入境检验检疫局综合技术服务中心
Publication Date
2022-07-15

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Abstract

The invention relates to the field of marine biotechnology, and discloses a rapid screening method for anemone toxin-producing microorganisms in view of the technical gap of a rapid screening method for anemone toxin-producing microorganisms, comprising the following steps: (1) obtaining strain DNA; ( 2) Obtain DNA probe hybridization solution: put the digoxigenin-labeled DNA probe solution in boiling water and then put it in an ice bath, take the DNA probe solution and add it to the hybridization solution; (3) Hybridization: place the DNA membrane Place in DNA probe hybridization solution and incubate for hybridization; place the hybridized DNA membrane in a container containing 2 x SSC solution for washing; (4) develop color; (5) judge the result. The DNA probe-colony in situ hybridization method is used for the rapid screening of sea anemone toxin-producing microbial strains, and the strains that can produce sea anemone toxin can be accurately screened through specific amplified DNA probes and simple and orderly operation steps , this method is simple and efficient, with short screening period and high accuracy.
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Description

technical field

[0001] The invention relates to the technical field of marine biology, in particular to a rapid screening method for anemone toxin-producing microorganisms. Background technique

[0002] Palytoxins (PLTXs) are one of the most toxic non-protein algal toxins known, and are listed as one of the largest non-polymeric natural products; they are highly toxic, fat-soluble, heat-stable polyether marine biotoxins ; is one of the most widely distributed and highest incidence marine toxins. It can cause irreversible damage to various organs and tissues such as the intestines, liver, nerves, placenta, etc., and there is no specific drug treatment after poisoning. Sea anemone toxins seriously endanger the safety of marine ecosystems and human health, and have become a worldwide research hotspot. Sea anemone toxin is a neurotoxin and has important applications in the detection of harmful red tide, neurophysiology, medical diagnosis, drug development, and biochemical warfa...

Claims

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