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A method for anti-protein adsorption on the hydrophobic surface of an oil-sealed gene sequencing chip

An anti-protein adsorption and gene sequencing technology, applied in the fields of gene sequencing and biochemistry, can solve problems such as giving too many prompts and immature

Active Publication Date: 2022-06-21
CYGNUS BIOSCI BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Third-generation sequencing technology is not yet mature due to its accuracy and other factors
Previous prior art did not give much hints in this direction

Method used

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  • A method for anti-protein adsorption on the hydrophobic surface of an oil-sealed gene sequencing chip
  • A method for anti-protein adsorption on the hydrophobic surface of an oil-sealed gene sequencing chip
  • A method for anti-protein adsorption on the hydrophobic surface of an oil-sealed gene sequencing chip

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Experimental program
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Effect test

Embodiment 1

[0045] The micro-pit array chip is composed of a chip panel with a micro-reaction chamber, double-sided tape, and glass. The chip panel with the micro-reaction chamber is etched from a fiber-optic panel or a silica-based panel, and the double-sided tape provides a fluid chamber for the hollow structure, as well as a fluid inlet and outlet. The upper surface of the micro-reaction chamber chip panel is modified with hydrophobic fluorosilane.

[0046] Prepare reagents. The aqueous reaction solution is: 2U / μL Bst DNA polymerase (1% protein by volume), 1mM manganese chloride, 100nM FAM dye (5-Carboxyfluorescein) dissolved in buffer (buffer is 20mM Tris-base , 20mM ammonium chloride, 50mM potassium chloride, pH 8.3). This aqueous phase reaction solution is a component of a conventional sequencing reaction solution, in which, in order to facilitate the observation of the oil sealing effect, the nucleotide substrate for sequencing is not added, and the fluorescent dye FAM is replace...

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Abstract

The invention discloses a method for anti-protein adsorption on the hydrophobic surface of an oil-sealed gene sequencing chip. By adding a fluoroalkyl surfactant in the solution, the hydrophobic end of the surfactant contains fluoroalkyl chains, and the hydrophilic end contains oligoethylene glycol groups. Compared with commonly used anti-protein adsorption reagents such as non-fluorinated surfactants and polyethylene glycol, fluoroalkyl surfactants can better resist protein adsorption. The anti-protein adsorption method disclosed in the present invention can be applied to high-throughput sequencing, digital PCR, digital ELASA and other high-throughput biological detection and the like.

Description

technical field [0001] The invention relates to a method for anti-protein adsorption on a hydrophobic surface of an oil-sealed gene sequencing chip, belonging to the field of biochemistry, and more precisely to the field of gene sequencing. Background technique [0002] Genetic sequencing is an emerging technology. Genetic safety and testing have received increasing attention in recent years. Among the existing gene sequencing technologies, next-generation sequencing technology is the mainstream sequencing technology. Third-generation sequencing technology has not yet matured due to factors such as its accuracy. According to predictions, the second-generation gene sequencing technology will be the mainstream of gene sequencing technology in the next ten years or even longer. Oil-sealed gene sequencing technology has many advantages, such as independent reaction chambers, non-intersecting reaction units and so on. In oil-sealed sequencing technology, surface treatment is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6869C12M1/34
CPCC12Q1/6869B01L3/5027B01L2200/141B01L2300/165C12Q2527/125C12Q2565/629C12Q2547/107
Inventor 陈子天刘燕乔硕段海峰李文涛
Owner CYGNUS BIOSCI BEIJING CO LTD