Method for detecting concentration of six bufadienolide components in toad skin-containing medicinal material preparation in blood plasma or tissue
A technology of bufastenolide and detection method, which is applied in material separation, measurement device, analysis material and other directions, can solve problems such as unloaded dried toad skin and the like, achieves simplified sample pretreatment process, excellent chromatographic peak shape, Simple to use effects
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[0065] (1) Preparation of stock solution:
[0066] Take the standard products Libufagenin, Bufalin, Cinobufagin, Cinobufalin, Cinobufagin, and Sandbufalin, respectively, and dissolve them in (50±5)% acetonitrile aqueous solution to prepare the standard stock solution; the standard stock solution is mixed to obtain a mixed standard stock solution;
[0067] Dissolve reserpine in (50±5)% acetonitrile aqueous solution to prepare reserpine stock solution;
[0068] (2) Preparation of working solution:
[0069] Dilute the mixed standard stock solution with (50 ± 5)% acetonitrile aqueous solution respectively to prepare a mixed standard curve sample working solution and a mixed quality control sample working solution;
[0070] Dilute the reserpine stock solution with (50 ± 5)% acetonitrile aqueous solution to prepare an internal standard working solution;
[0071] (3) Preparation of the sample solution to be tested:
[0072] Preparation of the plasma sample solution to be tested: ...
Embodiment 1
[0133] (1) Preparation of solution
[0134] Preparation of stock solutions:
[0135] Weigh an appropriate amount of standard products of the main components of toad skin medicinal materials, Bufagenin, Bufalin, Cinobufabufin, Cinobufafalin, Yuanhuabufajing, and Shabubufajing, and dissolve them in an appropriate amount of 50% acetonitrile In the aqueous solution, standard stock solutions with a concentration of 1.00 mg / mL were prepared. Three standard stock solutions with a concentration of 1.00 mg / mL were mixed in equal volumes, and an appropriate amount of 50% acetonitrile aqueous solution was added to prepare a mixed standard stock solution with a concentration of 0.100 mg / mL for the six bufacardenolactone components.
[0136] Take an appropriate amount of reserpine standard substance and dissolve it in 50% acetonitrile aqueous solution to prepare a reserpine stock solution with a concentration of 1.00 mg / mL.
[0137] Preparation of standard curve working solution:
[013...
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