High performance liquid chromatography tandem mass spectrometry detection method for refreshing liquid
A high-performance liquid chromatography and Qingnaofushen technology, which is applied in the field of drug analysis, can solve the problems of restricting the use of Qingnaofushen liquid and unclear quality standards, and achieve simplified sample pretreatment process, high sensitivity and rapid analysis Effect
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[0030] (1) Preparation of stock solution:
[0031] Take the standard products puerarin, paeoniflorin, naringin, hesperidin, baicalin, berberine, polygala saponin, baicalein, emodin, chrysophanol, and dissolve them in 100% methanol solution respectively to obtain the standard product stock solution; mix the standard stock solution to obtain a mixed standard stock solution;
[0032] (2) Preparation of standard curve solution:
[0033] Dilute the mixed standard stock solution with 40±5% acetonitrile aqueous solution respectively to prepare a mixed standard curve solution and a mixed quality control solution;
[0034] (3) Preparation of sample injection solution to be tested:
[0035] Preparation of sample solution to be tested: take Qingnao Fushen solution, add an appropriate amount of 40±5% acetonitrile aqueous solution to dilute to a suitable concentration, vortex to mix, and then pass through a 0.22 μm microporous membrane, the filtrate is the sample to be tested injection ...
Embodiment 1
[0106] (1) Preparation of solution
[0107] Preparation of stock solutions:
[0108] Weigh an appropriate amount of the standard products of puerarin, paeoniflorin, naringin, hesperidin, baicalin, berberine, polygala saponin, baicalein, emodin and chrysophanol, which are the main components of Qingnao Fushen Liquid, respectively. Dissolved in an appropriate amount of 100% methanol solution to prepare standard stock solutions with concentrations of 1.00 mg / mL, respectively.
[0109] Preparation of standard curve working solutions:
[0110] According to the method in Table 4 below, the standard stock solution of 10 components was diluted into a series of mixed standard curve solutions of C8-C1 with 40% acetonitrile aqueous solution;
[0111] Table 4
[0112]
[0113]
[0114] *Note: For convenience, the concentration of puerarin is used to replace the concentration of the mixed standard, and the proportion of the mixed system of the 10 analytes remains unchanged.
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