High performance liquid chromatography tandem mass spectrometry detection method for refreshing liquid

A high-performance liquid chromatography and Qingnaofushen technology, which is applied in the field of drug analysis, can solve the problems of restricting the use of Qingnaofushen liquid and unclear quality standards, and achieve simplified sample pretreatment process, high sensitivity and rapid analysis Effect

Pending Publication Date: 2022-07-19
HUACHENG PHARMA FACTORY GAUNGZHOU
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  • Application Information

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Problems solved by technology

The ambiguity of quality standards has greatly limited the use of Qingnao Fushen Liquid, so it is urgent to establish a highly sensitive, specific, fast and efficient analytical method for the active ingredients in Qingnao Fushen Liquid, so as to contribute to the development of Qingnao Fushen Liquid. Provide the basis for quality control and evaluation of Shenye, and provide technical support for the research of active ingredients in Qingnaofushenye

Method used

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  • High performance liquid chromatography tandem mass spectrometry detection method for refreshing liquid
  • High performance liquid chromatography tandem mass spectrometry detection method for refreshing liquid
  • High performance liquid chromatography tandem mass spectrometry detection method for refreshing liquid

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Experimental program
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preparation example Construction

[0030] (1) Preparation of stock solution:

[0031] Take the standard products puerarin, paeoniflorin, naringin, hesperidin, baicalin, berberine, polygala saponin, baicalein, emodin, chrysophanol, and dissolve them in 100% methanol solution respectively to obtain the standard product stock solution; mix the standard stock solution to obtain a mixed standard stock solution;

[0032] (2) Preparation of standard curve solution:

[0033] Dilute the mixed standard stock solution with 40±5% acetonitrile aqueous solution respectively to prepare a mixed standard curve solution and a mixed quality control solution;

[0034] (3) Preparation of sample injection solution to be tested:

[0035] Preparation of sample solution to be tested: take Qingnao Fushen solution, add an appropriate amount of 40±5% acetonitrile aqueous solution to dilute to a suitable concentration, vortex to mix, and then pass through a 0.22 μm microporous membrane, the filtrate is the sample to be tested injection ...

Embodiment 1

[0106] (1) Preparation of solution

[0107] Preparation of stock solutions:

[0108] Weigh an appropriate amount of the standard products of puerarin, paeoniflorin, naringin, hesperidin, baicalin, berberine, polygala saponin, baicalein, emodin and chrysophanol, which are the main components of Qingnao Fushen Liquid, respectively. Dissolved in an appropriate amount of 100% methanol solution to prepare standard stock solutions with concentrations of 1.00 mg / mL, respectively.

[0109] Preparation of standard curve working solutions:

[0110] According to the method in Table 4 below, the standard stock solution of 10 components was diluted into a series of mixed standard curve solutions of C8-C1 with 40% acetonitrile aqueous solution;

[0111] Table 4

[0112]

[0113]

[0114] *Note: For convenience, the concentration of puerarin is used to replace the concentration of the mixed standard, and the proportion of the mixed system of the 10 analytes remains unchanged.

[01...

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Abstract

The invention relates to a high performance liquid chromatography-tandem mass spectrometry detection method for a refreshing and refreshing liquid. The high performance liquid chromatography-tandem mass spectrometry detection method is used for detecting the concentrations of 10 active ingredients, namely puerarin, paeoniflorin, naringin, hesperidin, baicalin, berberine, tenuifolin, baicalein, emodin and chrysophanol, in the refreshing and refreshing liquid. Comprising the following steps: (1) preparing a stock solution: respectively dissolving each effective component in a 100% methanol solution to prepare a standard stock solution; (2) preparing a to-be-detected sample solution; and (3) performing liquid chromatography separation and mass spectrometry on the to-be-detected sample solution obtained in the step (2). The detection method provided by the invention has the advantages of excellent chromatographic peak shape, simple operation, rapid analysis, high specificity and high separation degree, and the channel of each compound only has a unique target peak.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a high-performance liquid chromatography-tandem mass spectrometry detection method for Qingnao Fushen liquid, and more particularly, to a high-performance liquid chromatography-tandem mass spectrometry detection method for the concentrations of 10 main components in Qingnao Fushen liquid medicine method. Background technique [0002] Qingnao Fushen Liquid is listed in the ninth volume of Chinese medicine prescription preparations issued by the Ministry of Health. , Baiziren, Uncaria, Polygala, Pinellia, Campanulaceae, Zhuru, Chenpi, Pueraria, Qianghuo, Angelica and other 48 traditional Chinese medicines. It has the functions of clearing the mind and calming the nerves, removing phlegm and refreshing the brain, promoting blood circulation and dredging collaterals, and is mainly used for neurasthenia, insomnia, intractable headache, headache caused by post-concussion sequela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02G01N30/86
CPCG01N30/06G01N30/02G01N30/8634
Inventor 陈志钊殷玮周兰华张志坚宫剑平戚园梅彭佳裕
Owner HUACHENG PHARMA FACTORY GAUNGZHOU
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