Linear polyfunctional multimer biomolecule coupled to polyubiquitin linker and use thereof

A biomolecular, multifunctional technology for fusion with degradation motifs, peptides containing affinity tags, biochemical equipment and methods, etc., to solve problems such as separation or analytical efficiency limitations
CN112204046APending Publication Date: 2021-01-08ONEGENE BIOTECH INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ONEGENE BIOTECH INC
Publication Date
2021-01-08

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Abstract

The present invention provides a linear multimer biomolecule polymer wherein a biomolecule is bonded to a polyubiquitin scaffold formed of two or more covalently bonded ubiquitins, by obtaining, froma host cell, a biomolecule bonded with a ubiquitin C-terminal tag through recombinant expression, and polyubiquitinating the biomolecule in vitro in the presence of proteins involved in ubiquitination, E1 (activation enzyme), E2 (conjugation enzyme), and E3 (ligase), and a substrate. The polymer according to the present invention may be used in the separation and purification of a biomolecule, theseparation of a target material that binds to the biomolecule, etc.
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Description

technical field

[0001] The present invention relates to methods for preparing biomolecules comprising proteins as polymers in the form of multimers. In particular, the present invention relates to methods for preparing recombinantly expressed biomolecules from host cells into linear multifunctional multimeric biomolecular polymers using the ubiquitination system. Background technique

[0002] Biomolecules and / or smallmolecule chemical compounds including proteins, peptides, polypeptides, antibodies, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) ) in a multimeric form has several advantages. For example, two or more homogeneous or heterogeneous proteins are fused (fusion) or a cross-linker (cross linker or cross-linking agent) is used to link two or more homogeneous or heterogeneous proteins, thereby improving protein Physical and chemical properties such as solubility, gelation, thermal stability and pH stability. For example, when starch oxidation is carried out...

Claims

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