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Application of s100a11 protein as a diabetes biomarker and therapeutic target

A technology of S100A11 and protein expression, which is applied in the application field of diabetes biomarkers and therapeutic targets, can solve the problems of unfavorable and unreported S100A11 relationship, and achieve the effect of promoting insulin resistance

Active Publication Date: 2021-12-28
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Interestingly, S100A11 is low-expressed in esophageal cancer and bladder cancer, and the loss of S100A11 is highly correlated with poor prognosis in the course of bladder cancer and ovarian cancer
At present, the research on the function of S100A11 protein mainly focuses on osteoarthritis, various tumors and metabolism, and there is no report on the relationship between S100A11 and diabetes

Method used

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  • Application of s100a11 protein as a diabetes biomarker and therapeutic target
  • Application of s100a11 protein as a diabetes biomarker and therapeutic target
  • Application of s100a11 protein as a diabetes biomarker and therapeutic target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 8-week-old spontaneous diabetic model leptin receptor-deficient mice (db / db) and the same-week-old wild-type control mice (purchased from Nanjing Model Animal Center) were selected and fed with normal diet for 4 weeks. The expression of S100A11 in RNA level and protein level in liver of db / db mice was detected respectively.

[0040] It was found that the mRNA and protein expression of S100A11 in db / db liver was higher than that of control mice ( figure 1 ).

Embodiment 2

[0042] Construct liver S100A11-specific overexpression of adeno-associated virus AAV-8 (purchased from Weizhen Biotech), with 2*10 11 v.g / tail vein injection into C57BL / 6 mice. Insulin tolerance test (ITT) was performed on the 15th day. The mice were fasted for 6 hours, and 1.25 U per kilogram of body weight was injected intraperitoneally. Blood was collected from the tail vein at 0, 15, 30, 60, 90, and 120 minutes to detect blood glucose. Glucose tolerance test (GTT) was performed on the 20th day. The mice were fasted for 16 hours, and 1.5 g of D-glucose was injected intraperitoneally per kilogram of body weight. Blood was collected from the tail vein at 0, 15, 30, 60, 90, and 120 minutes to detect blood glucose.

[0043] It was found that compared with control mice, AAV overexpression of S100A11 showed a significant decrease in insulin sensitivity. Impaired glucose tolerance ( figure 2 ).

Embodiment 3

[0045] The body weight of the mice injected with GFP adeno-associated virus and S100A11 adeno-associated virus was monitored. The mice were dissected on the 24th day after virus injection, and the liver and various adipose tissues were weighed.

[0046] The results showed that the body weight of the mice in the S100A11 overexpression group increased significantly, and the liver, epididymal fat and subcutaneous fat all increased significantly ( image 3 ).

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Abstract

The present invention provides the application of S100A11 protein as a diabetes biomarker and therapeutic target. The present invention studies the expression of S100A11 in leptin receptor deficient (db / db) spontaneous diabetic mice, and finds that S100A11 protein can promote Insulin resistance, increased accumulation of lipid droplets in the liver and increased liver and fat weight. After the inventors compared the control group and the experimental group, there was a significant difference in the expression level of S100A11. Therefore, S100A11 can be used as a marker for diabetes diagnosis, prognosis assessment or drug screening.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to the application of S100A11 protein as a diabetes biomarker and therapeutic target. Background technique [0002] Accelerated urbanization and industrialization, and the increasing westernization of people's lifestyles and diets have led to a sharp rise in the incidence of obesity. Obesity is one of the key factors of chronic metabolic diseases, and non-alcoholic fatty liver disease (NAFLD), diabetes mellitus (T2DM), and cardiovascular diseases are rapidly increasing. Diabetes is a metabolic disorder caused by genetic and environmental factors, and is closely related to the body's insulin resistance and glucose and lipid metabolism disorders. Insulin resistance refers to a pathophysiological state in which physiological doses of insulin mediate the decrease in sensitivity of target organs (liver, muscle and fat) and the weakening of biological effects. However, due to the unclear ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P3/10C12Q1/6883G01N33/68
CPCA61K38/1738A61P3/10C12Q1/6883G01N33/6893C12Q2600/158G01N2800/042G01N2333/4727
Inventor 张惠杰滕菲
Owner SOUTHERN MEDICAL UNIVERSITY
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