Ternary metal Pd-M-Ir nano-enzyme as well as preparation method and application thereof

A ternary metal, pd-m-ir technology, applied in the field of metal nanozymes, can solve the problems of inability to combine hydrogen peroxide molecules, weak activity, low hydrogen peroxide adsorption, etc., and achieve the effect of improving sensitivity

Inactive Publication Date: 2021-01-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] As a new type of inorganic material that can replace biological enzymes, metal nanozymes have their own characteristics: for example, they can have the function of biological enzymes, and can be placed stably at room temperature for a long time without inactivation; but they also have some defects of their own: such as The adsorption en

Method used

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  • Ternary metal Pd-M-Ir nano-enzyme as well as preparation method and application thereof
  • Ternary metal Pd-M-Ir nano-enzyme as well as preparation method and application thereof
  • Ternary metal Pd-M-Ir nano-enzyme as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a method for preparing a ternary metal Pd-Ru-Ir nanozyme, comprising the steps of: (1) preparing a mother nucleus, (2) preparing a Pd-Ru system, (3) preparing a Pd-Ru-Ir nanozyme . Specific steps are as follows:

[0044] (1) Preparation of mother nucleus

[0045] 110 mg of PVP, 65 mg of ascorbic acid, 600 mg of potassium bromide were weighed into a round bottom flask, and 8 mL of water was added. Preheat in an oil bath at 80°C, then add 3mL (19mg / mL) Na to the round bottom flask 2 PdCl 4Aqueous solution, the reaction was continued for 3h (reaction temperature 80°C) and the reaction was terminated. The reaction solution was centrifuged and washed 3 times with water at a speed of 12000rpm to collect the reaction product (mother nucleus), and then the reaction product was dispersed in ethylene glycol to prepare a colloidal solution of the mother nucleus with a Pd concentration of 2mg / mL.

[0046] (2) Preparation of Pd-Ru system

[0047] Weigh...

Embodiment 2

[0051] This embodiment provides a method for preparing a ternary metal Pd-Rh-Ir nanozyme, comprising steps: (1) preparing a mother nucleus, (2) preparing a Pd-Rh system, (3) preparing a Pd-Rh-Ir nanozyme . Specific steps are as follows:

[0052] (1) Preparation of mother nucleus

[0053] 110 mg of PVP, 65 mg of ascorbic acid, 600 mg of potassium bromide were weighed into a round bottom flask, and 8 mL of water was added. Preheat in an oil bath at 80°C, then add 3mL (19mg / mL) Na to the round bottom flask 2 PdCl 4 Aqueous solution, the reaction was continued for 3h (reaction temperature 80°C) and the reaction was terminated. The reaction solution was centrifuged and washed 3 times with water at a speed of 12000rpm to collect the reaction product (mother nucleus), and then the reaction product was dispersed in ethylene glycol to prepare a colloidal solution of the mother nucleus with a Pd concentration of 2mg / mL.

[0054] (2) Preparation of Pd-Rh system

[0055] Weigh the P...

Embodiment 3

[0059] This embodiment provides a method for preparing a ternary metal Pd-Au-Ir nanozyme, comprising the steps of: (1) preparing a mother nucleus, (2) preparing a Pd-Au system, (3) preparing a Pd-Au-Ir nanozyme . Specific steps are as follows:

[0060] (1) Preparation of mother nucleus

[0061] 110 mg of PVP, 65 mg of ascorbic acid, 600 mg of potassium bromide were weighed into a round bottom flask, and 8 mL of water was added. Preheat in an oil bath at 80°C, then add 3mL (19mg / mL) Na to the round bottom flask 2 PdCl 4 Aqueous solution, the reaction was continued for 3h (reaction temperature 80°C) and the reaction was terminated. The reaction solution was centrifuged and washed 3 times with water at a speed of 12000rpm to collect the reaction product (mother nucleus), and then the reaction product was dispersed in ethylene glycol to prepare a colloidal solution of the mother nucleus with a Pd concentration of 2mg / mL.

[0062] (2) Preparation of Pd-Au system

[0063] Weig...

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Abstract

The invention relates to the technical field of metal nano-enzymes, in particular to a ternary metal Pd-M-Ir nano-enzyme, a preparation method(M = Ru, Rh, Au)of the ternary metal Pd-M-Ir nano-enzyme,and application of the ternary metal Pd-M-Ir nano-enzyme in immunoassay. The preparation method comprises the following steps: reducing sodium tetrachloropalladate with ascorbic acid to prepare a parent nucleus of a metal nano-enzyme; and reacting the parent nucleus with a metal M salt to prepare a Pd-M system, and reacting the Pd-M system with an iridium salt at a high temperature to form the ternary metal Pd-M-Ir nano-enzyme. The ternary metal Pd-M-Ir nano-enzyme has good tolerance to acid, base and temperature, especially the ternary metal PdRuIr nano-enzyme, can replace horse radish peroxidase to be applied to immunohistochemistry. And an idea is provided for improving immunohistochemical sensitivity.

Description

technical field [0001] The invention relates to the technical field of metal nanozymes, in particular to a ternary metal Pd-M-Ir nanozyme (M=Ru, Rh, Au), and a preparation method and method for the ternary metal Pd-M-Ir nanozyme The application of the ternary metal nanometer Pd-M-Ir in immunoassay. Background technique [0002] As a new type of inorganic material that can replace biological enzymes, metal nanozymes have their own characteristics: for example, they can have the function of biological enzymes, and can be placed stably at room temperature for a long time without inactivation; but they also have some defects of their own: such as The adsorption energy for hydrogen peroxide is low, resulting in its weak activity. The reason is mainly that the hydroxyl radicals formed in the catalytic process can be more stably adsorbed on the surface of the nanozyme, and other hydrogen peroxide molecules cannot be well combined with the active sites on the nanozyme, thereby Inh...

Claims

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Application Information

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IPC IPC(8): B01J23/46B01J23/52G01N33/535
CPCB01J23/468B01J23/52B01J35/0013B01J35/006G01N33/535
Inventor 张雷王卫国李杉颜金武
Owner SOUTH CHINA UNIV OF TECH
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