A kind of tea saponin thiosemicarbazone zinc complex and its preparation method and application
The technology of aglycone peptide amino and zinc complex is applied in the field of medicine, and can solve the problems of only considering antibacterial activity, no report on research, and no consideration of immune function regulation effect, etc., and achieves improvement of immune enhancement effect, simple preparation process, The effect of facilitating industrial production
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Embodiment 1
[0035] A preparation method of tea saponin thiosemicarbazone zinc complex, comprising the steps of:
[0036] (1) Theosaponin (C 30 h 48 o 6 ) 10g mixed with 10g thiosemicarbazide, added 100g of absolute ethanol, added 1g of glacial acetic acid, refluxed at 60°C for 10h, and evaporated the solvent under reduced pressure to obtain product 1 (11.5g).
[0037] (2) Dissolve 10 g of product 1 in 100 g of methanol, add 1 g of 0.5% zinc acetate methanol solution, reflux at 50° C. for 10 h, cool to room temperature, filter, and the solid is the final product (11.0 g).
Embodiment 2
[0039] A preparation method of tea saponin thiosemicarbazone zinc complex, comprising the steps of:
[0040] (1) Theosaponin (C 30 h 48 o 6 ) 10g was mixed with 30g of thiosemicarbazide, 300g of absolute ethanol was added, 0.5g of glacial acetic acid was added, refluxed at 90°C for 5h, and the solvent was distilled off under reduced pressure to obtain product 1 (12.6g).
[0041] (2) Dissolve 10 g of product 1 in 300 g of methanol, add 0.5 g of a mass concentration of 5% zinc chloride methanol solution, reflux at 80° C. for 5 h, cool to room temperature, filter, and the solid is the final product (10.5 g) .
Embodiment 3
[0043] A preparation method of tea saponin thiosemicarbazone zinc complex, comprising the steps of:
[0044] (1) Theosaponin (C 30 h 48 o 6 ) 10g mixed with 20g of thiosemicarbazide, added 200g of absolute ethanol, added 0.8g of glacial acetic acid, refluxed at 70°C for 8h, evaporated the solvent under reduced pressure, and obtained product 1 (11.8g).
[0045] (2) Dissolve 10 g of product 1 in 200 g of methanol, add 0.8 g of a mass concentration of 3% zinc sulfate in methanol, reflux at 70° C. for 8 h, cool to room temperature, filter, and the solid is the final product (10.7 g).
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