Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

P-hydroxyphenylalanine urine detection reagent magnetic nanoparticles and preparation method thereof

A technology of hydroxyphenylalanine and magnetic nanometers, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of low convenience, non-portability, low stability, etc., to improve detection speed and dissolution speed , the effect of improving stability

Pending Publication Date: 2021-01-12
南京迪安医学检验所有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the market, mercury sulfate solution, mercury nitrate solution, nickel sulfate solution, α-nitroso-β-naphthol solution are generally prepared separately, and then mixed in proportion. This operation is complicated and requires a laboratory with complete equipment. Complete, if it is configured in advance and made into test strips, the stability will be low and the accuracy of the urine test will not be high. The longer the test reagent is stored, the color change will not be obvious, and the accuracy will be lower, so it cannot be portable and must be carried out in the laboratory. , the convenience is low, and the detection quality is reduced; the market needs a method that can enhance the stability of the detection reagent, improve the storage time limit of the detection reagent, and make it convenient to make a portable detection device; the present invention solves such problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • P-hydroxyphenylalanine urine detection reagent magnetic nanoparticles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] A preparation method of p-hydroxyphenylalanine urine detection reagent magnetic nano-microspheres, comprising the steps of:

[0050] Prepare materials according to recipes;

[0051] The formula includes: 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nanoparticles, 60-120 parts of absolute ethanol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of α-nitroso-β-naphthol, 64-120 parts of surfactant;

[0052] Making mercury sulfate magnetic nanospheres:

[0053] Step 1, microsphere polymer shell substrate:

[0054]Dissolve the gelatin in water at 80-90 degrees Celsius and stir, add acid-soluble chitosan into the gelatin solution and stir, then stir and add magnetic nanoparticles, and finally add absolute ethanol, stir and dissolve at a temperature of 20-40 degrees Celsius, Degassing and aging under a vacuum of 0.06-0.08MPa to obtain a polymer shell substrate;

[0055] Step...

Embodiment 1

[0081] Embodiment 1: gelatin 26g, acid-soluble chitosan 18g, iron oxide nanoparticles 0.6g, dehydrated alcohol 60g, mercuric sulfate 12g, the concentration that collocates is 1% secondary alkyl sodium sulfate 24g, mercurous nitrate 56g, collocation The concentration is 32g of 1% secondary alkyl sodium sulfate, 2g of nickel sulfate, 4g of 1% secondary alkyl sodium sulfate, 1g of α-nitroso-β-naphthol, and 1% of Secondary alkyl sodium sulfate 4g.

Embodiment 2

[0082] Embodiment 2: gelatin 34g, acid-soluble chitosan 26g, iron oxide nanoparticles 0.8g, dehydrated alcohol 90g, mercuric sulfate 18g, the concentration that collocates is 1% secondary alkyl sodium sulfate 38g, mercurous nitrate 62g, collocation The concentration is 48g of 1% secondary alkyl sodium sulfate, 4g of nickel sulfate, 8g of 1% secondary alkyl sodium sulfate, 2g of α-nitroso-β-naphthol, and 1% of Secondary alkyl sodium sulfate 6g.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses p-hydroxyphenylalanine urine detection reagent magnetic nanoparticles and a preparation method thereof. The p-hydroxyphenylalanine urine detection reagent magnetic nanoparticles are prepared from 26-42 parts of gelatin, 18-34 parts of acid-soluble chitosan, 0.6-1.2 parts of magnetic nano particles, 60-120 parts of absolute ethyl alcohol, 12-24 parts of mercury sulfate, 56-68 parts of mercurous nitrate, 2-6 parts of nickel sulfate, 1-3 parts of alpha-nitroso-beta naphthol and 64-120 parts of surfactant. According to the preparation method, each reagent is independently wrapped by the microsphere polymer shell base material, so that the stability of the detection reagent can be improved; and a complex preparation process is not needed, the reagent can be used for detection after being mixed with acid liquor, and can be made into portable detection equipment, and the accuracy of urinalysis is improved.

Description

technical field [0001] The invention relates to the field of detection reagents, in particular to a p-hydroxyphenylalanine urine detection reagent magnetic nano-microsphere and a preparation method thereof. Background technique [0002] "P-Hydroxyphenylalanine Urine Detection Reagent" is mainly used to qualitatively detect the content of p-Hydroxyphenylalanine in urine, so as to judge the metabolic activity of malignant tumor cells in the human body, and then evaluate the risk of malignant tumors. Abnormal amino acid metabolism is common in patients with malignant tumors, that is, the process of protein decomposition into amino acids is enhanced, while the catabolism of amino acids is weakened, so that amino acids can be reused for tumor cell protein synthesis, and excess and unusable amino acids are excreted. Abnormal amino acid metabolism exists in the early stage of tumorigenesis, and one of the important manifestations is the elevated content of p-hydroxyphenylalanine (t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574G01N33/68G01N33/543G01N33/545
CPCG01N33/57488G01N33/6812G01N33/54346G01N33/54326
Inventor 周翰林
Owner 南京迪安医学检验所有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com