Application of combination of CXCL10 and HGF as pneumonia and infection source detection marker

A marker and infection source technology, applied in the field of immune detection, to achieve the effect of convenient use

Inactive Publication Date: 2021-01-15
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report of which inflammatory factors can be clearly used as clinical diagnostic biomarkers for the detection of pneumonia and its source of infection

Method used

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  • Application of combination of CXCL10 and HGF as pneumonia and infection source detection marker
  • Application of combination of CXCL10 and HGF as pneumonia and infection source detection marker
  • Application of combination of CXCL10 and HGF as pneumonia and infection source detection marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Detection of CXCL10 and HGF gene mRNA expression levels in blood samples by real-time fluorescent quantitative PCR

[0047] Whole blood samples were collected from 20 pneumonia patients diagnosed with viral infection, bacterial infection, and virus-bacterial cross-infection and 20 normal healthy people as controls.

[0048] Featuring PureLink from Thermo Fisher Scientific TM Total RNA Blood kit (K156001) was used to extract total RNA from each blood sample according to the operating instructions. Use Thermo Scientific NanoDrop 2000 to measure the mRNA concentration in each sample, and then use Promega's GoScript TM Reverse Transcriptase (A5001) was used to synthesize cDNA, followed by The qPCR Master Mixture (A6001) of Green I dye was used to quantitatively analyze the mRNA level of myosin 1b in the blood of suspected pneumonia patients and healthy people, and the GAPDH gene was used as an internal reference gene. According to real-time fluorescence quant...

Embodiment 2

[0058] Example 2 Using enzyme-linked immunosorbent assay (ELISA) kits to detect the expression levels of CXCL10 and HGF proteins in the plasma samples of patients with pneumonia

[0059]Collect plasma samples from 20 patients diagnosed with viral infection, bacterial infection, virus and bacterial cross-infection pneumonia, and 20 normal healthy people as controls, and then use Abcam’s Human CXCL10 and HGF ELISA kits, according to the kits provided by the manufacturer. The instructions measure the CXCL10 and HGF protein content in plasma of normal healthy group, virus infected group, bacterial infected group and cross-infected virus and bacteria. Relative multiples, and do statistical analysis.

[0060] Result analysis: if image 3 As shown, compared with healthy normal people, the plasma CXCL10 in patients with pneumonia caused by viral infection was significantly increased, and HGF was not significantly changed; the plasma HGF in patients with pneumonia caused by bacterial ...

Embodiment 3

[0061] Example 3 Utilize colloidal gold test strips to detect CXCL10 and HGF biomarkers

[0062] Colloidal gold test strips based on dual detection lines of CXCL10 and HGF antibodies, each test strip contains nitrocellulose membrane, sample pad, binding pad, absorbent paper and PVC plate support, the nitrocellulose membrane package of the test strip The binding pad contains mouse anti-human CXCL10 monoclonal antibody and mouse anti-human HGF monoclonal antibody labeled with colloidal gold. Cloned antibody, so the test strip has quality control line C, CXCL10 detection line T1 and HGF detection line T2, such as Figure 4 shown. The detection operation process is as follows: before the detection, put the sample to be tested, the detection reagent and other detection materials at room temperature, take out the test card and place it flat on the table, absorb the sample to be tested with a straw, and add 2 to 3 drops of the sample to the test On the card, observe the display res...

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Abstract

The invention provides an application of a combination of CXCL10 and HGF as a pneumonia and infection source detection marker. Specifically, whether a corresponding subject suffers from pneumonia or not can be identified and the infection source (bacterial, virus and virus bacterial cross infection) which causes the pneumonia can be distinguished by detecting the expression level (whether the expression level is high or not) of mRNA expression quantity and/or protein quantity of CXCL10 and HGF genes in a whole blood/plasma sample, so that a user can easily and conveniently judge whether he orshe needs to see a doctor or not. Particularly, under the condition of insufficient medical conditions, a reasonable treatment scheme can be made in time according to the illness degree and the type of infection source of the patient. Correspondingly, the invention further provides a reagent or a kit suitable for real-time quantitative fluorescent PCR detection, an enzyme-linked immunosorbent assay kit, a test strip based on detection of CXCL10 and HGF and the like.

Description

technical field [0001] The invention belongs to the technical field of immunodetection, and relates to a detection marker for identifying bacteria, viruses, and pneumonia caused by virus-bacteria cross-infection, and predicting pneumonia patients and infection sources. Background technique [0002] Pneumonia is a serious lung infection disease. It is an inflammation of the alveoli caused by bacteria, viruses, fungi and other microorganisms. The alveoli are filled with fluid or pus (purulent substance), causing cough, accompanied by sputum or pus, Symptoms include fever, chills, and difficulty breathing. Left undiagnosed and treated, it can rapidly deteriorate and lead to other diseases such as heart failure, empyema, lung abscess, myocarditis, or toxic encephalitis. Seriously affect the life safety of infants and young children, the elderly over 65 years old and people with weakened immune systems. About 450 million people worldwide are infected with pneumonia every year, o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68G01N33/577G01N33/558
CPCC12Q1/6883G01N33/6893G01N33/6863G01N33/74G01N33/577G01N33/558C12Q2600/158G01N2333/522G01N2333/4753G01N2800/12G01N2800/26G01N2800/7095
Inventor 熊裕焱于怡陈富林牛芳林王迓君闫媛
Owner NORTHWEST UNIV(CN)
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