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Construction method and application of novel PLS3 gene knockout rat animal model

A construction method, gene knockout technology, applied in the field of biomedicine, can solve the problem of not fully interpreting the phenotype of the disease

Active Publication Date: 2021-01-22
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

Although some studies have established a PLS3 mutant mouse model, this model cannot fully explain the phenotype of the disease (Neugebauer J, Heilig J, Hosseinibarkooie S, et al. Plastin 3 influences bone homeostasis through regulation of osteoclast activity[J]. Hum Mol Genet ,2018,27(24):4249-4262)

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  • Construction method and application of novel PLS3 gene knockout rat animal model
  • Construction method and application of novel PLS3 gene knockout rat animal model
  • Construction method and application of novel PLS3 gene knockout rat animal model

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[0075] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings. The following examples are only used to illustrate the present invention, and are not intended to limit the scope of the present invention. It should be noted that the spirit and form of the technical solution of the present invention can be modified or replaced without departing from the subject and scope of the present invention, but these modifications and replacements all belong to the protection scope of the present invention.

[0076] Example PLS3 Gene Targeted Mutation Rat Model Construction and Genotype Identification

[0077] The present invention utilizes CRISPR / Cas9 technology to carry out large fragment targeted knockout of exons 10-16 (E10-E16) of the PLS3 gene [Rattus norvegicus (Norway rat)]. The reference sequence is the PLS3 gene sequence (SEQID NO.17), the information comes from the NCBI database (NCBI Gene ID: 81748, http...

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Abstract

The invention discloses a construction method and application of a novel PLS3 gene knockout rat animal model. The construction method comprises the following steps of firstly, performing targeted knockout on E10-E16 fragments of a PLS3 gene in a cell to obtain a PLS3 gene mutation cell, and then constructing a novel PLS3 gene knockout animal model by utilizing the obtained cell. The novel animal model constructed by the construction method makes up the defects of osteoporosis genetic related animal models, and provides the reliable, effective and novel experimental animal model for researchinga genetic mechanism of osteoporosis, and the important application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a construction method and application of a novel PLS3 gene knockout rat animal model. Background technique [0002] Osteoporosis is an age-related systemic bone disease characterized by decreased bone strength and increased fracture risk. The epidemiological report released by the National Health and Health Commission in 2018 showed that the prevalence of osteoporosis among people over the age of 50 in China was as high as 19.2%, and the prevalence of osteoporosis was high and still increasing rapidly (Chinese Medical Association Osteoporosis Branch of Osteoporosis and Bone Mineral Diseases, China Osteoporosis Epidemiological Survey and "Healthy Bones" Special Action Results Released [J]. Chinese Journal of Osteoporosis and Bone Mineral Diseases, 2019,12(4):317- 318). The most serious consequence of osteoporosis is fragility fractures, which can cause limited mo...

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Application Information

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IPC IPC(8): A01K67/027C12N15/85C12N15/12C12N5/10
CPCA01K67/0276C12N15/85C07K14/47A01K2217/075A01K2227/105A01K2267/0331A01K2267/03
Inventor 李梅夏维波胡静孙磊
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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