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Method for simultaneously detecting three mycotoxins based on time-resolved fluorescence labeling-aptamer recognition

A time-resolved fluorescence and mycotoxin technology, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and analytical materials, can solve the problems of rare applications of time-resolved fluorescent aptasensors, improve accuracy and stability, improve sensitivity, The effect of long fluorescence lifetime

Inactive Publication Date: 2021-01-22
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, only a few WS-based 2 Bioassay methods for nanosheets have been developed, mostly based on WS 2 Quenching of fluorescent dyes, which is rarely used in time-resolved fluorescent aptasensors

Method used

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  • Method for simultaneously detecting three mycotoxins based on time-resolved fluorescence labeling-aptamer recognition
  • Method for simultaneously detecting three mycotoxins based on time-resolved fluorescence labeling-aptamer recognition
  • Method for simultaneously detecting three mycotoxins based on time-resolved fluorescence labeling-aptamer recognition

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Experimental program
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Effect test

Embodiment 1

[0031] Zearalenone, T-2 toxin and aflatoxin B in corn samples 1 Detection and spiked recovery

[0032] 1. Amine functionalized Ln 3+ doped KYF 4 Preparation of nanoparticles

[0033] ln 3+ (Ln 3+ = Dy 3+ , Tb 3+ and Eu 3+ ) doped KYF 4 Nanoparticles were prepared by a facile one-step solvothermal method: KCl (2.0 mmol), YCl 3 (1.0mmol), the required amount of LnCl 3 (0.01mmol Dy 3+ , 0.1 mmol Tb 3+ and 0.1 mmol Eu 3+ ) and PEI (1 mL) were added to ethylene glycol (20 mL) and mixed well. The above solution was then added to the 4 F (6.0mmol) in ethylene glycol (20mL) solution. Continue to stir the reaction at room temperature for 30 min, then transfer the mixture into a 50 mL autoclave, and react at 200°C for 4 hours. After the reaction was completed, the reactor was taken out to cool down to room temperature naturally, and the nanoparticles were collected by centrifugation, washed three times with ultrapure water and absolute ethanol in turn, and the obtained p...

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Abstract

The invention provides a method for simultaneously detecting three mycotoxins based on time-resolved fluorescence labeling aptamer recognition, and belongs to the technical field of nano materials andmolecular biology. The method specifically comprises the following steps of: modifying time-resolved fluorescent nanoparticles with biotinylated zearalenone aptamers AptZEN, T-2 toxin aptamers AptT-2and aflatoxin B1 aptamers AptAFB1 respectively to obtain KYF4: Dy<3+>- AptZEN, KYF4: Tb<3+>-AptT2 and KYF4: Eu<3+>-AptAFB1 fluorescent probes, and detecting the fluorescence signal intensity of the mixture by using tungsten disulfide nanosheets as a fluorescence quenching agent. The method can be used for detecting zearalenone, T-2 toxin and aflatoxin B1 in samples such as corn, wheat, grain, feed and products thereof.

Description

technical field [0001] The invention belongs to the field of nanometer material and molecular biotechnology. In particular, it relates to a simultaneous detection of zearalenone, T-2 toxin and aflatoxin B1 using time-resolved fluorescent nanomaterials and tungsten disulfide nanosheets combined with nucleic acid aptamer recognition. The strong fluorescence and long fluorescence lifetime of fluorescent nanomaterials, the high fluorescence quenching efficiency of tungsten disulfide nanosheets, and the high affinity and specificity of nucleic acid aptamers to targets effectively avoid the interference of sample biological background fluorescence and improve the sensitivity of detection and accuracy, and can correctly identify three mycotoxins at the same time. Background technique [0002] Mycotoxins are naturally occurring chemical metabolites during fungal growth and are considered the most important chronic dietary risk factors. Mycotoxin contamination can occur at any stag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N2021/6432
Inventor 王周平索碧娅孙羽菡俞晔
Owner JIANGNAN UNIV
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