Preparation method and application of magnetic exosome-entrapped refractory wound dressing

An exosome, magnetic technology, applied in pharmaceutical formulations, pharmaceutical science, drug delivery, etc., to achieve the effects of good temperature responsiveness, wide range of sources, good biodegradability and biosafety

Pending Publication Date: 2021-01-29
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, thermosensitive hydrogel dressings loaded with magnetic exosomes have not been reported yet.

Method used

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  • Preparation method and application of magnetic exosome-entrapped refractory wound dressing
  • Preparation method and application of magnetic exosome-entrapped refractory wound dressing
  • Preparation method and application of magnetic exosome-entrapped refractory wound dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Preparation of Thermosensitive Hydrogel Encapsulating Magnetic Exosomes

[0068] According to the volume ratio of chitosan solution: β-sodium glycerophosphate solution: hydroxyethyl cellulose solution of 4:1:2, the temperature-sensitive hydrogel containing magnetic exosomes was prepared. The schematic diagram of the preparation process is shown in figure 1 .

[0069] 1. Preparation of magnetic exosomes (M-Exo)

[0070] (1) When human bone marrow mesenchymal stem cells are cultured to 80% confluency, replace with exosome-free medium;

[0071] (2) Adding Fe 3 o 4 Superparamagnetic nanoparticles, so that the final concentration of nanoparticles is 0.5mg / mL, continue to cultivate;

[0072] (3) After culturing for 72 hours, collect the cell supernatant, and centrifuge at 2000×g for 10 minutes to remove dead cells and impurities;

[0073] (4) Take the supernatant and centrifuge at 10,000×g for 10 minutes to remove cell debris;

[0074] (5) Continue to take the...

Embodiment 2

[0085] Example 2 Preparation of Thermosensitive Hydrogel Encapsulating Magnetic Exosomes

[0086] According to the volume ratio of chitosan solution: β-glycerophosphate sodium solution: hydroxyethyl cellulose solution is 6:1:2, the temperature-sensitive hydrogel containing magnetic exosomes is prepared. The schematic diagram of the preparation process is shown in figure 1 .

[0087] 1. Preparation of magnetic exosomes (M-Exo)

[0088] (1) When human bone marrow mesenchymal stem cells are cultured to 80% confluency, replace with exosome-free medium;

[0089] (2) Adding Fe 3 o 4 Superparamagnetic nanoparticles, so that the final concentration of nanoparticles is 0.5mg / mL, continue to cultivate;

[0090] (3) After culturing for 72 hours, collect the cell supernatant, and centrifuge at 2000×g for 10 minutes to remove dead cells and impurities;

[0091] (4) Take the supernatant and centrifuge at 10,000×g for 10 minutes to remove cell debris;

[0092] (5) Continue to take the su...

Embodiment 3

[0103] Example 3 Preparation of Thermosensitive Hydrogel Encapsulating Magnetic Exosomes

[0104] According to the volume ratio of chitosan solution: β-glycerophosphate sodium solution: hydroxyethyl cellulose solution is 7:1:2, the temperature-sensitive hydrogel containing magnetic exosomes is prepared. The schematic diagram of the preparation process is shown in figure 1 .

[0105] 1. Preparation of magnetic exosomes (M-Exo)

[0106] (1) When human bone marrow mesenchymal stem cells are cultured to 80% confluency, replace with exosome-free medium;

[0107] (2) Adding Fe 3 o 4 Superparamagnetic nanoparticles, so that the final concentration of nanoparticles is 0.5mg / mL, continue to cultivate;

[0108] (3) After culturing for 72 hours, collect the cell supernatant, and centrifuge at 2000×g for 10 minutes to remove dead cells and impurities;

[0109] (4) Take the supernatant and centrifuge at 10,000×g for 10 minutes to remove cell debris;

[0110] (5) Continue to take the ...

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Abstract

The invention discloses a preparation method and application of a magnetic exosome-entrapped refractory wound dressing. The preparation method comprises the following steps of firstly, mixing a chitosan solution with a betasodium glycerophosphate solution to obtain a mixed solution I; then adding a hydroxyethyl cellulose solution into the mixed solution I to obtain a mixed solution II; co-incubating the human mesenchymal stem cells and the Fe3O4 superparamagnetic nanoparticles, extracting to obtain magnetic exosomes, and adding the magnetic exosomes into the mixed solution II to prepare the temperature-sensitive hydrogel dressing entrapping the magnetic exosomes. The hydrogel dressing prepared by the method has excellent temperature sensitivity, can be injected for administration, has theeffects of resisting bacteria, promoting angiogenesis and the like, and has a good application prospect in promotion of refractory wound repair.

Description

technical field [0001] The invention belongs to the technical field of degradable biomedical materials, and in particular relates to a preparation method and application of a biologically active refractory wound dressing. Background technique [0002] With the development of my country's social economy, population aging and changes in people's lifestyles, the spectrum of chronic diseases in society has also changed. The high incidence of geriatric diseases and metabolic diseases has gradually increased the incidence of chronic refractory wounds. Among them, the prevalence of diabetes among people aged 20 to 79 is increasing rapidly, and it is estimated that the prevalence of diabetes will reach 7.7% (approximately 439 million) by 2030. Diabetic foot ulcers caused by diabetes are a typical complication of diabetes, which manifests as protracted and difficult-to-heal wounds, and eventually develops into chronic non-healing wounds, which can lead to amputation and even life-thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0061A61L26/0052A61L26/0057A61L26/009A61L2300/404A61L2300/236A61L2300/30A61L2300/412A61L2300/41A61L2400/06A61L2300/64C08L5/08C08L1/286
Inventor 王海康琳吴志宏邱贵兴
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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