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Method for preparing functional hydrolysate by degrading eggshell membrane by using steam explosion technology

An egg shell membrane and functional technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of acid vapor and waste liquid adverse effects, environmental protection, high cost, etc.

Inactive Publication Date: 2021-01-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is relatively not environmentally friendly, and the acid vapor and waste liquid produced have great adverse effects on the human body and the environment.
[0005] 2. The reduction method uses a reducing agent to reduce the disulfide bond in the eggshell membrane protein molecule to a sulfhydryl group, thereby obtaining a soluble protein, but the sulfhydryl compound reducing agent has a strong pungent smell and high cost
[0008] At present, there is no report on the application of steam explosion technology to the degradation of shell membranes and the preparation of functional hydrolysates

Method used

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  • Method for preparing functional hydrolysate by degrading eggshell membrane by using steam explosion technology
  • Method for preparing functional hydrolysate by degrading eggshell membrane by using steam explosion technology
  • Method for preparing functional hydrolysate by degrading eggshell membrane by using steam explosion technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] The method for preparing functional hydrolyzate 1 by degrading egg shell membranes by steam explosion technology comprises the following steps:

[0135] 1) Steam explosion treatment

[0136] Weigh 1000g and put it in the steam explosion machine reactor, keep the pressure at 1.4MPa for 0.5min, then release the pressure instantly for 0.1s, collect the steam explosion samples, and store them at -4°C for later use;

[0137] 2) The sample is degraded by dilute alkali method

[0138] The steam explosion sample was added into NaOH solution with an alkali concentration of 0.6% according to a solid-liquid ratio of 1:25, and the temperature was 60°C, and the reaction was shaken for 0.5h, filtered to obtain a filtrate, and freeze-dried to obtain a functional hydrolyzate 1.

Embodiment 2

[0140] The method for preparing functional hydrolyzate 2 by degrading egg shell membranes using steam explosion technology comprises the following steps:

[0141] 1) Steam explosion treatment

[0142] Weigh 1000g of eggshell membranes and place them in a steam explosion machine reactor, hold the pressure at 1.6MPa for 1min, then release the pressure instantly for 0.1s, collect the steam explosion samples, store them at -4°C, and keep them for later use;

[0143] 2) The sample is degraded by dilute alkali method

[0144] Add the steam explosion sample into NaOH solution with an alkali concentration of 0.4% according to the ratio of solid to liquid 1:20, and shake the reaction for 1 h at a temperature of 25°C, filter to obtain the filtrate, and freeze-dry to obtain functional hydrolyzate 2.

Embodiment 3

[0146] The method for preparing functional hydrolyzate 3 by degrading egg shell membranes by steam explosion technology comprises the following steps:

[0147] 1) Steam explosion treatment

[0148] Weigh 1000g of eggshell membranes and place them in a steam explosion machine reactor, hold the pressure at 1.8MPa for 2min, then release the pressure instantly for 0.1s, collect the steam explosion samples, and store them at -4°C for later use;

[0149] 2) The sample is degraded by dilute alkali method

[0150] Add the steam explosion sample into NaOH solution with an alkali concentration of 0.2% according to the ratio of solid to liquid 1:15, and shake the reaction for 2 hours at a temperature of 40°C, filter to obtain the filtrate, and freeze-dry to obtain functional hydrolyzate 3.

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Abstract

The invention discloses a method for preparing a functional hydrolysate by degrading an eggshell membrane by using a steam explosion technology, which comprises the following steps: putting the eggshell membrane into a steam explosion machine reactor, carrying out pressure maintaining treatment for 0.5-5 minutes under the pressure of 0.5-2.2 MPa, instantaneously releasing the pressure, collectinga steam explosion sample, adding the steam explosion sample into a NaOH solution, at a temperature of 25 to 60 DEG C, carrying out a reaction for 0.5-4 hours under oscillation, filtering to obtain filtrate, and carrying out freeze drying to obtain the functional hydrolysate. Compared with the existing reduction method, enzymolysis method and steam explosion assisted dilute alkali solution degradation method, the method provided by the invention has the advantages of simple operation process, short treatment time, high efficiency and easiness in industrial production.

Description

technical field [0001] The invention relates to the field of egg by-product processing, in particular to a method for preparing functional hydrolyzate by degrading egg shell membranes using steam explosion technology. Background technique [0002] As a by-product of human life, eggshells exist in large quantities, and the eggshell membrane attached to them is particularly likely to cause great environmental pollution. In fact, eggshell membranes are the same potential protein and amino acid resources as the main by-products of the animal product processing industry: horns, hooves, skins, and hairs. The eggshell membrane contains about 90% protein, 2% carbohydrate and 3% liposome. The proteins in the shell membrane mainly include keratin, collagen (mostly I, V, X types) and composite proteins, among which keratin and collagen are more abundant. If these discarded eggshell membranes are fully utilized, economic benefits can be increased and environmental pollution can be avo...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/34
CPCC07K1/145C07K1/34
Inventor 付星张源超黄茜邱宁蔡朝霞金永国靳国锋盛龙马美湖
Owner HUAZHONG AGRI UNIV
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