Fermentation medium and fermentation method of cordyceps sinensis mycelium with improved polysaccharide content
A fermentation medium and a technology for Cordyceps sinensis, which are applied to the fermentation medium and fermentation field of Cordyceps sinensis mycelium, can solve the problems of low content of active components, long cultivation period, uneven quality of Cordyceps sinensis mycelium, etc. The effect of increasing the content and shortening the culture period
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Embodiment 1
[0057] Embodiment 1: the preparation of insect protein
[0058] A: Starve the 4-5 instar bat moth larvae for 12 hours, then treat them in 100°C water for 60 seconds, smash the bat moth larvae, adjust the pH value to 6-7, add papain by 3% by weight, and stir well , carry out enzymatic hydrolysis at 60°C, and carry out coarse filtration after 10 minutes of enzymatic hydrolysis, here, use a 20-mesh sieve to filter, and the filtrate should have no lumps. After filtering, the enzymolyzate was treated at 100°C for 8 minutes to inactivate the enzyme, and after the treatment, it was dried and powdered to obtain insect protein.
[0059] B: Starve the 4-5 instar bat moth larvae for 12 hours, then treat them in 100°C water for 60 seconds, smash the bat moth larvae, adjust the pH value to 6-7, add papain by 3% by weight, and stir evenly , carry out enzymatic hydrolysis at 60°C, and carry out coarse filtration after 30 minutes of enzymatic hydrolysis, here, use a 20-mesh sieve to filter, ...
Embodiment 2
[0063] Embodiment 2: the preparation of fermentation medium
[0064] Mix the insect protein prepared in Example 1, glucose, yeast powder, potassium dihydrogen phosphate and magnesium sulfate, add water under stirring, and its weight ratio is: insect protein / glucose yeast powder / potassium dihydrogen phosphate / magnesium sulfate / water = 50 / 10 / 10 / 2 / 1 / 200, the mixed mixture was kept at 40° C. for 40 minutes of fermentation, and then sterilized at high temperature to obtain the fermentation medium.
[0065] According to Example 1, different insect proteins are prepared to obtain different fermentation media, and the fermentation media prepared by insect protein A is A c , the fermentation medium prepared by insect protein B is B c , the fermentation medium prepared by insect protein C is C c , the fermentation medium prepared by insect protein D is D c , the fermentation medium prepared by insect protein E is E c .
Embodiment 3
[0066] Embodiment 3: the fermentation of Cordyceps sinensis mycelium
[0067] Carry out the fermentation of Cordyceps sinensis mycelium according to the following method:
[0068] (1) Inoculate the liquid strain of Cordyceps sinensis (according to CN100374542C, prepared in the embodiment steps 1 and 2 in the asexual stage continuous fermentation process of Cordyceps sinensis) to the fermentation medium prepared according to Example 2 (A c , B c , C c , D c ,E c ), inoculate 5 mL of Cordyceps sinensis liquid strain inoculated with 50 mL of fermentation medium, cultivate at 16° C. for 1 day at 150 rpm, then add 0.005% indole acetic acid by medium weight, and ferment at 13° C. for 2 days to obtain Cordyceps sinensis bacterial liquid.
[0069] (2) Step (1) Cordyceps sinensis bacterium liquid is added to the fermentation medium prepared according to Example 2 (A c , B c , C c ,D c ,E c ), 5 mL of Cordyceps sinensis bacteria liquid was inoculated into 100 mL of fermentation...
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