96 results about "Insect Proteins" patented technology

The invention relates to the technical field of intelligent manufacturing, in particular to an insect protein extraction automatic production line and quality tracing method based on machine vision, which comprises the following steps of: acquiring an image and pressure data advancing time sequence, comparing a path and pressure fluctuation, identifying an abnormal section image frame, extracting a boundary and gray scale difference, and marking a structure defect. And matching action time, sorting station abnormity, combining a frame sequence and a chamber number, searching a traceability table record, updating an abnormal state field, and forming a quality traceability result set. According to the method, an image particle path and a pressure intensity signal are propelled in parallel in a time dimension, the synchronous recognition capability of image change and a physical state is enhanced, a structural anomaly judgment basis is constructed by particle boundary pixels and gray scale difference, and image time and a push rod action form uninterrupted binding through natural superposition; receiving frames and cavity serial numbers are classified into a unified serial number logic according to a time sequence, abnormal identifiers and channel information are embedded into batch fields in parallel, images are communicated, and abnormal tracing and positioning are carried out.

The invention discloses a method for producing edible fungi, insect protein and bio-organic fertilizer by utilizing navel orange industry wastes, which comprises the following steps: step 1, crushing collected navel orange residues and navel orange trees into fragments, with the solid content of the navel orange residues being 24% and the solid content of the navel orange trees being 36%; 2, the solid waste obtained in the step 1 is mixed with 10% wheat bran, wherein the ratio of the navel orange residues to the navel orange trees to the wheat bran is 3: 6: 1; 3, the organic mixed base material obtained in the step 2 is disinfected and then used for edible mushroom planting; and 4, breeding hermetia illucens by using the waste base material obtained in the step 3 to obtain the insect protein and the bio-organic fertilizer. And a circular economy concept is realized, and the utilization of resources is maximized.

The invention provides an edible fungus culture medium and a preparation method, and relates to the technical field of edible fungus cultivation, and the edible fungus culture medium comprises the following components by mass: 10-20 parts of a seaweed polysaccharide matrix, 5-15 parts of insect protein powder, 15-25 parts of biological carbon fiber, 10-20 parts of corncob powder, 0.5-2 parts of a natural silicon source, a proper amount of a trace element solution, and a proper amount of water. The algal polysaccharide matrix is obtained by extracting algal polysaccharides, including algin or alginic acid, from algae in hot water, the insect protein powder is prepared by drying, crushing and carrying out enzymolysis on yellow mealworms or fly maggots, the biochar fibers are prepared by pyrolyzing bamboo or crop straws, and the natural silicon source is diatomite, bentonite or perlite; the particle size of the natural silicon source is micron-sized, and the trace element solution contains zinc, iron, copper and manganese elements. The culture medium has the beneficial effects that the problems of unbalanced nutrition, poor physical performance, weak disease resistance and the like of the culture medium in the prior art are effectively solved.

The invention relates to the technical field of intelligent manufacturing, in particular to a visual intelligent monitoring method and system for an insect protein extraction process, and the method comprises the following steps: collecting insect protein extraction process data, calculating time evolution and key conformation change, and generating extraction path data; the method comprises the following steps: calculating final conformation stability and entropy change by using principal component analysis, generating an entropy change curve, inputting a hidden Markov model to calculate a state transition probability, generating a tag sequence, performing anomaly detection based on the tag sequence and entropy, identifying an abnormal conformation time period, analyzing and improving experimental parameters, and generating an insect protein visual monitoring result. According to the method, the sensitivity and decision support capability of a monitoring system are improved by capturing an insect protein extraction track in real time, analyzing a conformation evolution process, quantifying entropy change and revealing an energy change trend, recognizing a state transition rule in combination with a probability model, screening abnormal conformation and tracing experimental variables, and formulating an optimized feedback combination scheme.

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

The invention belongs to the technical field of solenopsis invicta prevention and control, and particularly relates to solenopsis invicta food bait as well as a preparation method and application thereof. The invention provides red imported fire ant food bait. The red imported fire ant food bait is prepared from energy main materials and auxiliary materials, the energy main material consists of the following components in percentage by weight: 50-70% of insect protein powder, 5-20% of corn flour, 9-11% of peanut oil, 9-11% of fish meal and 9-11% of cane sugar. The invention also provides an attractant matched with the red imported fire ant food bait. According to the invention, the efficiency of finding bait by the red imported fire ants can be improved, meanwhile, the recruitment behaviors of the worker ants are further increased, more worker ants are lured, and the comprehensive trapping effect is good.

The present invention relates to a method for artificial rejuvenation of black soldier flies, comprising: raising black soldier flies indoors for multiple generations, clarifying the number of degeneration generations and degradation indicators thereof, analyzing the evolutionary sources through gene analysis of new black soldier fly populations from different places, and determining comparative advantage traits; selecting populations with target traits, hybridizing with degraded varieties to obtain F1 generation seed worms, and artificially screening to obtain black soldier fly varieties with stable target traits after colonization of composite microbial agents and feeding target livestock and poultry feces. This method solves the problem of degeneration of black soldier flies raised for multiple generations, and compared with the original strain, the efficiency of black soldier fly larvae in processing livestock and poultry feces is significantly improved after artificial rejuvenation, the larval development period is shortened, and the quality of larvae is improved, meeting the needs of large-scale processing of livestock and poultry feces and producing insect protein using black soldier flies, and has good economic and social benefits.

The invention provides a preparation method for making 3D printing snacks based on insect protein powder. The preparation method comprises the following steps: mixing degreased insect protein powder with a thermal stability tackifier; adding composite liquid containing glycerol and sodium citrate, and shearing to form gel-like paste; a preliminary thermal response structure is constructed through low-temperature curing; carrying out layer-by-layer deposition printing by utilizing temperature control food 3D printing equipment; and after printing, normal-temperature standing is performed to realize structure self-stabilization. According to the preparation method disclosed by the invention, the compound polysaccharide and the protein structure stabilizer are combined for use, a supporting framework with thermal response crosslinking capability is introduced in a raw material mixing stage, and a gel-like paste with viscoelasticity and rheological stability is formed by matching a compound liquid containing sodium citrate and a controlled shearing technology; and preliminary establishment of a three-dimensional microstructure network is realized through low-temperature curing, so that the anti-bleeding capability and the thermal structure maintaining capability of the paste in the heating and deposition processes are effectively improved, and the problems of line fracture and interlayer separation in a traditional scheme are avoided.

The invention discloses an application of ultrasound and teuvincenone H diterpenoid treatment in reduction of tenebrio molitor protein allergy, the tenebrio molitor protein is treated by ultrasound and teuvincenone H diterpenoid, so that the binding capacity of the tenebrio molitor protein and immunoglobulin E (IgE) can be reduced, and the allergy of the tenebrio molitor protein is further reduced. The invention discloses other effects of teuvincenone H diterpene except for anti-tumor and anti-inflammatory effects for the first time, and the method has the advantages of simple process steps, short treatment time, low temperature, high efficiency, rapidness and environmental friendliness, is beneficial to wide application of related technologies in the field of food industry, and provides a brand new scheme for developing safe and high-quality insect protein food.

The present invention provides methods for controlling Ostrinia furnacalis and protecting crops, in particular corn, from economic damage caused by Ostrinia furnacalis. The invention further relates to the use of plants stably transformed with nucleic acid molecules encoding the Cry proteins of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Ostrinia furnacalis.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of gastrointestinal cancer crowds. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, porridge, vegetable juice, fruit juice, oat powder, coix seed powder, black bean powder, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of gastrointestinal cancer people, the real intestinal environment of domestic gastrointestinal cancer people is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the gastrointestinal cancer people can be constructed. The method is beneficial to accurate diagnosis and personalized treatment of intestinal microecology of people with gastrointestinal cancer, and better evaluates the health state of people with gastrointestinal cancer and formulates preventive measures.

The present invention provides a method for controlling Ostrinia furnacalis (Ostrinia furnacalis) and protecting a crop, in particular corn, from economic damage caused by the Ostrinia furnacalis (Ostrinia furnacalis). The invention further relates to the use of a plant stably transformed with a nucleic acid molecule encoding a Cry protein of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Asiatic Ostrinia furnacalis.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of people with hyperuricemia. The culture medium contains yeast extract, ethanol, adenine, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructose, glucose, inulin, resistant dextrin, dietary fiber, oxalic acid, saturated fatty acid, sialic acid, peanut oil, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, cysteine hydrochloride and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of people suffering from hyperuricemia, the real intestinal environment of people suffering from hyperuricemia in China is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of people suffering from hyperuricemia can be constructed. The method is beneficial to accurate diagnosis of intestinal microecology of people with hyperuricemia, individualized treatment of antisense booking and disease mechanisms, better maintenance of intestinal health and formulation of preventive measures.

The invention relates to the technical field of insect resource utilization, and discloses a comprehensive utilization method for extracting protein and grease from insects, which comprises the following steps: selecting hermetia illucens as a raw material, crushing, adding water with the weight 2.5-3.5 times that of the raw material, and stirring and mixing; grinding the mixture to 40-120 microns through bone paste grinding equipment; adding chitinase, lipase and protease which account for 0.015-0.035% of the total amount, and carrying out enzymolysis at 52-58 DEG C for 3.5-6.5 hours; raising the temperature to 90-100 DEG C and carrying out enzyme deactivation for 25-35 minutes; homogenizing by a colloid mill until the particle size is 3-15 microns; solid residues, a grease phase and a liquid protein phase are separated out through a disc type centrifugal machine; carrying out spray drying on the liquid protein phase to obtain degreased insect protein powder with moisture less than 6%; and standing and layering the oil phase, and filtering with a 0.2-1.2 micron filter membrane to obtain the refined insect oil with the acid value of less than 2mgKOH / g. According to the invention, the quality and nutritional value of the protein product are improved, the equipment blockage risk and energy consumption are reduced, and the applicability of the grease food-grade standard is improved.

The invention provides a functional feed additive for improving the quality of tilapia mossambica. The functional feed additive comprises the following raw materials: hydrolyzed fish meal, fermented soybean meal, insect protein powder, fish soluble, callicarpa nudiflora powder, fish oil, polysaccharide, an intestine regulating agent and vitamins. The preparation method of the hydrolyzed fish meal comprises the following steps: crushing fish, adding a Na2CO3 solution for rinsing, performing high-temperature and high-pressure squeezing for degreasing, adding a mixed enzyme solution for enzymolysis, performing enzyme deactivation for 2-3 times, performing centrifugal separation, concentrating, drying, crushing and sieving. Wherein the polysaccharide is fucoidin and trametes robiniophila polysaccharide, and the intestine regulating agent is lactobacillus rhamnosus powder and lactobacillus kefir powder. The feed additive is balanced in formula nutrition, improves the quality of tilapia mossambica, reduces intestinal diseases of tilapia mossambica, and has application value.

A process and product obtainable by acid hydrolysis of insects, for example from cricket powder. As one application, an acid hydrolysis product made from powdered crickets may be used as a seasoning sauce resembling soy sauce. The insect powder is hydrolyzed with acid, followed by neutralization, and optionally clarification. In comparison to the typical production of soy sauce, the acid hydrolysis procedure uses insect protein as the main substrate or even the sole substrate. The flavor of soy sauce-like products made from acid-hydrolyzed cricket protein results from a complex combination of compounds generated by the reactions.

The application discloses a straw ant cultivation method, which comprises the following steps: plot selection, land preparation, ant nest composite planting, straw feeding and mushroom management; the straw ant cultivation method is based on the biological ecology principle of ant foraging behavior, aims at creating suitable microorganisms, temperature and humidity microenvironment under different environments, and providing straw food to increase the foraging efficiency of ants, so as to increase the efficiency of straw decomposition of the ant nest system; in addition, the straw ant cultivation method can help to relieve the agricultural environmental problems of straw recycling, increase the yield of wild chicken conk and ant insect protein, and create economic value with high added value.

The invention relates to a kitchen garbage recycling method which comprises the following steps: pretreating kitchen garbage to obtain a kitchen garbage oil phase, a kitchen garbage liquid phase and a kitchen garbage solid phase; placing the eggs on the surface of wheat bran for hatching to obtain larvae; inoculating the larvae on the surface of the kitchen waste solid phase for breeding, and adding solid residues to obtain a solid mixture; and separating the solid mixture to obtain adults and fecula. The kitchen garbage is fed by insects and is converted into various high-value products: the insect protein can replace conventional feeds such as fish meal and soybean meal, so that the pressure of feed resources is relieved; the insect fat can be used for producing biodiesel or cosmetic raw materials; the insect sand is a high-quality organic fertilizer and can effectively improve the soil fertility. And finally, efficient conversion and sustainable cyclic utilization of the kitchen garbage are realized.

The present invention provides a preparation method for 3D printing snacks using insect protein powder. It comprises the following steps: mixing defatted insect protein powder with a heat-stable thickening agent; adding a composite liquid containing glycerol and sodium citrate, and shearing to form a gel-like paste; forming an initial thermoregulatory structure through low-temperature curing; using a temperature-controlled food 3D printing device for layer-by-layer deposition printing; after printing, allowing it to stand at room temperature to achieve self-stabilization of the structure.

The invention discloses a method for preparing insect protein peptide by utilizing bacillus velezensis JAAS-22. The method comprises the following steps: (1) performing amplification culture on a strain of the bacillus velezensis JAAS-22 to obtain a bacillus velezensis JAAS-22 fermentation culture solution; (2) homogenizing insect larvae in a colloid mill to obtain insect pulp; (3) uniformly mixing the insect pulp with the bacillus velezensis JAAS-22 fermentation culture solution, and carrying out enzymolysis fermentation to obtain an insect pulp enzymolysis fermentation product; and (4) dialyzing the enzymatic hydrolysis fermentation product of the insect pulp to obtain the insect protein peptide with the molecular weight less than or equal to 30 kDa. According to the method, the bioactive peptide with stable and good antibacterial activity can be efficiently prepared.

The present invention is related to a method for the manufacture of a product that imitates properties of meat, poultry, fish, seafood, or products derived therefrom, comprising the step of dosing and depositing a first and optionally a second material onto a surface, preferably in a cavity (19), of a product carrier (16), preferably a mold, wherein at least one of said first material and second material comprises cellular medium, and at least one said first material and second material comprises a protein, preferably a vegetable protein, insect protein, cell protein including single cell protein, such as of yeast, bacteria, microalgae, fungi, fermented products, mold and the like, or a mixture of different proteins, wherein the protein content in the dry raw materials is 35 to 90%, preferably greater than 50% and is particularly preferably in the range from 60% to 90%.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The present invention is related to a method for the manufacture of a product that imitates properties of meat, poultry, fish, seafood, dairy products such as cheese or yoghurt, or products derived therefrom, comprising the step of dosing and depositing a first and optionally a second material onto a surface, preferably in a cavity (19), of a product carrier (16), preferably a mold, wherein at least one said first material and second material comprises a protein, preferably a vegetable protein, insect protein, cell protein including single cell protein, such as of yeast, bacteria, microalgae, fungi, fermented products, mold and the like, or a mixture of different proteins, wherein the protein content in the dry raw materials is 35 to 90%, preferably greater than 50% and is particularly preferably in the range from 60% to 90%.

The present invention provides a modified Cry1Ac protein having enhanced toxicity to resistant Pink Bollworm (PBW) species. Insertion of the specific, closely bound intestinal peptides at one or more recognition sites in Cry1Ac increases retention of toxins in the PBW intestine, resulting in dissolution and pore formation, thereby causing sepsis and larva death. The modified Bt toxins show significant toxicity to resistant Pink Bollworm and thus can be used to produce insect resistant transgenic plants.

The present application provides a camel-derived anti-insect protein and its coding gene and application, and belongs to the field of genetic engineering and biological control; the nucleotide and amino acid sequences of the anti-insect protein are shown in SEQ IN NO. 1 and SEQ IN NO. 2 respectively, the anti-insect protein is a single-domain antibody with insecticidal activity screened from a camel-derived nanobody library, has small molecular weight, is simple to prepare, and is derived from a llama, is used for agricultural pest control, and has important scientific and practical significance for expanding new insecticidal gene resources.

The invention provides aquarium fish feed for gullfish and a preparation method of the aquarium fish feed, and relates to the technical field of aquatic product feeding. The aquarium fish feed is prepared from hermetia illucens larva powder, white fish meal, haematococcus pluvialis wall-broken powder, lycium barbarum polysaccharide extract, squid viscera hydrolysate, compound vitamin premix, mineral premix, yeast beta-glucan, Chinese herbal medicine extract, compound probiotics and sodium alginate. According to the invention, the defects in the prior art are overcome, the insect protein powder is used for replacing fish meal / cattle heart, natural color enhancing components are added, and probiotics and Chinese herbal medicines are combined for compounding, so that the synergistic effect of nutrition, environmental protection and functionality is realized, the growth of the symphysodon is effectively promoted, and water pollution is reduced.

The invention belongs to the technical field of aquatic feed application, and discloses a feed for improving the body color of litopenaeus vannamei. The addition of the hermetia illucens ointment provides rich essential amino acids and trace elements for the litopenaeus vannamei, which are key factors for the formation of the body color and the appearance quality of the litopenaeus vannamei. The method is crucial for improving the buying desire of consumers and the market competitiveness of products, the economic value of the litopenaeus vannamei is directly increased, and the overall economic benefit is improved. Due to the environment-friendly characteristic of the hermetia illucens ointment, the feed formula disclosed by the invention is also beneficial to improving the growth efficiency and health level of the prawns. Nutrient components in the hermetia illucens ointment are easy to digest and absorb, the prawns can better utilize the nutrients to support the growth and development of the prawns, and the resistance of the prawns to diseases is enhanced. By adding the insect protein hermetia illucens paste, the use amount of the fish meal is reduced, the feed cost is effectively reduced, and the feed shows significant benefits in improving the body color and appearance quality of the litopenaeus vannamei.

This disclosure provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences encoding insecticidal proteins. Specifically, the nucleic acid sequences can be used to prepare plants and microorganisms with insecticidal activity. Therefore, this disclosure provides transformed bacteria, plants, plant cells, plant tissues, and seeds. The compositions are insecticidal nucleic acids and proteins of bacterial strains. The sequences can be used to construct expression vectors for subsequent transformation into organisms of interest, including plants, and as probes for isolating other homologous (or partially homologous) genes. The insecticidal proteins can be used to control, kill, or inhibit the growth of the following pest populations: Lepidoptera, Coleopteran, Dipteran, fungi, Hemipteran, and nematodes, and can also be used to produce compositions with insecticidal activity.

The utility model belongs to the technical field of supercritical extraction, and particularly relates to a supercritical extraction device suitable for insect protein production, which comprises an extraction kettle, the top of the extraction kettle is fixedly connected with a servo motor; the transmission end of the servo motor is fixedly connected with a rotating shaft; the bottom end of the rotating shaft is rotationally connected to the bottom of the inner side wall of the extraction kettle; the outer side wall of the rotating shaft is rotationally connected with a first rotating frame. The side wall, close to the extraction kettle, of the first rotating frame is slidably connected with a fixed plate; a plurality of sections of fixing plates are arranged on the side wall of the first rotating frame; according to the step, a first rotating frame, a fixing plate, a brush and a spring are arranged to form an extraction kettle internal cleaning structure, the function of cleaning the inner side wall of the extraction kettle is achieved, the problem that the extraction kettle needs to be opened and other tedious steps are needed when the extraction kettle is cleaned is solved, the cleanliness of the inner side wall of the extraction kettle is improved, residual materials attached to the inner side wall of the extraction kettle are reduced, and the production efficiency is improved. And the cleaning operation steps are simplified.

Compositions and methods for controlling corn plant pests are disclosed. In particular, novel insecticidal proteins that are toxic against coleopteran and / or lepidopteran insect pests are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Also disclosed are methods of making the insecticidal proteins and methods of using the insecticidal proteins as well as nucleic acids encoding the insecticidal proteins of the invention, e.g., to confer protection from insect damage in transgenic maize plants.