78 results about "Insect Proteins" patented technology

The invention relates to the technical field of intelligent manufacturing, in particular to an insect protein extraction automatic production line and quality tracing method based on machine vision, which comprises the following steps of: acquiring an image and pressure data advancing time sequence, comparing a path and pressure fluctuation, identifying an abnormal section image frame, extracting a boundary and gray scale difference, and marking a structure defect. And matching action time, sorting station abnormity, combining a frame sequence and a chamber number, searching a traceability table record, updating an abnormal state field, and forming a quality traceability result set. According to the method, an image particle path and a pressure intensity signal are propelled in parallel in a time dimension, the synchronous recognition capability of image change and a physical state is enhanced, a structural anomaly judgment basis is constructed by particle boundary pixels and gray scale difference, and image time and a push rod action form uninterrupted binding through natural superposition; receiving frames and cavity serial numbers are classified into a unified serial number logic according to a time sequence, abnormal identifiers and channel information are embedded into batch fields in parallel, images are communicated, and abnormal tracing and positioning are carried out.

The invention relates to the technical field of intelligent manufacturing, in particular to a visual intelligent monitoring method and system for an insect protein extraction process, and the method comprises the following steps: collecting insect protein extraction process data, calculating time evolution and key conformation change, and generating extraction path data; the method comprises the following steps: calculating final conformation stability and entropy change by using principal component analysis, generating an entropy change curve, inputting a hidden Markov model to calculate a state transition probability, generating a tag sequence, performing anomaly detection based on the tag sequence and entropy, identifying an abnormal conformation time period, analyzing and improving experimental parameters, and generating an insect protein visual monitoring result. According to the method, the sensitivity and decision support capability of a monitoring system are improved by capturing an insect protein extraction track in real time, analyzing a conformation evolution process, quantifying entropy change and revealing an energy change trend, recognizing a state transition rule in combination with a probability model, screening abnormal conformation and tracing experimental variables, and formulating an optimized feedback combination scheme.

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

The invention belongs to the technical field of solenopsis invicta prevention and control, and particularly relates to solenopsis invicta food bait as well as a preparation method and application thereof. The invention provides red imported fire ant food bait. The red imported fire ant food bait is prepared from energy main materials and auxiliary materials, the energy main material consists of the following components in percentage by weight: 50-70% of insect protein powder, 5-20% of corn flour, 9-11% of peanut oil, 9-11% of fish meal and 9-11% of cane sugar. The invention also provides an attractant matched with the red imported fire ant food bait. According to the invention, the efficiency of finding bait by the red imported fire ants can be improved, meanwhile, the recruitment behaviors of the worker ants are further increased, more worker ants are lured, and the comprehensive trapping effect is good.

The invention provides a preparation method for making 3D printing snacks based on insect protein powder. The preparation method comprises the following steps: mixing degreased insect protein powder with a thermal stability tackifier; adding composite liquid containing glycerol and sodium citrate, and shearing to form gel-like paste; a preliminary thermal response structure is constructed through low-temperature curing; carrying out layer-by-layer deposition printing by utilizing temperature control food 3D printing equipment; and after printing, normal-temperature standing is performed to realize structure self-stabilization. According to the preparation method disclosed by the invention, the compound polysaccharide and the protein structure stabilizer are combined for use, a supporting framework with thermal response crosslinking capability is introduced in a raw material mixing stage, and a gel-like paste with viscoelasticity and rheological stability is formed by matching a compound liquid containing sodium citrate and a controlled shearing technology; and preliminary establishment of a three-dimensional microstructure network is realized through low-temperature curing, so that the anti-bleeding capability and the thermal structure maintaining capability of the paste in the heating and deposition processes are effectively improved, and the problems of line fracture and interlayer separation in a traditional scheme are avoided.

The present invention provides methods for controlling Ostrinia furnacalis and protecting crops, in particular corn, from economic damage caused by Ostrinia furnacalis. The invention further relates to the use of plants stably transformed with nucleic acid molecules encoding the Cry proteins of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Ostrinia furnacalis.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of gastrointestinal cancer crowds. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, porridge, vegetable juice, fruit juice, oat powder, coix seed powder, black bean powder, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of gastrointestinal cancer people, the real intestinal environment of domestic gastrointestinal cancer people is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the gastrointestinal cancer people can be constructed. The method is beneficial to accurate diagnosis and personalized treatment of intestinal microecology of people with gastrointestinal cancer, and better evaluates the health state of people with gastrointestinal cancer and formulates preventive measures.

The present invention provides a method for controlling Ostrinia furnacalis (Ostrinia furnacalis) and protecting a crop, in particular corn, from economic damage caused by the Ostrinia furnacalis (Ostrinia furnacalis). The invention further relates to the use of a plant stably transformed with a nucleic acid molecule encoding a Cry protein of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Asiatic Ostrinia furnacalis.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of people with hyperuricemia. The culture medium contains yeast extract, ethanol, adenine, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructose, glucose, inulin, resistant dextrin, dietary fiber, oxalic acid, saturated fatty acid, sialic acid, peanut oil, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, cysteine hydrochloride and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of people suffering from hyperuricemia, the real intestinal environment of people suffering from hyperuricemia in China is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of people suffering from hyperuricemia can be constructed. The method is beneficial to accurate diagnosis of intestinal microecology of people with hyperuricemia, individualized treatment of antisense booking and disease mechanisms, better maintenance of intestinal health and formulation of preventive measures.

The invention relates to the technical field of insect resource utilization, and discloses a comprehensive utilization method for extracting protein and grease from insects, which comprises the following steps: selecting hermetia illucens as a raw material, crushing, adding water with the weight 2.5-3.5 times that of the raw material, and stirring and mixing; grinding the mixture to 40-120 microns through bone paste grinding equipment; adding chitinase, lipase and protease which account for 0.015-0.035% of the total amount, and carrying out enzymolysis at 52-58 DEG C for 3.5-6.5 hours; raising the temperature to 90-100 DEG C and carrying out enzyme deactivation for 25-35 minutes; homogenizing by a colloid mill until the particle size is 3-15 microns; solid residues, a grease phase and a liquid protein phase are separated out through a disc type centrifugal machine; carrying out spray drying on the liquid protein phase to obtain degreased insect protein powder with moisture less than 6%; and standing and layering the oil phase, and filtering with a 0.2-1.2 micron filter membrane to obtain the refined insect oil with the acid value of less than 2mgKOH / g. According to the invention, the quality and nutritional value of the protein product are improved, the equipment blockage risk and energy consumption are reduced, and the applicability of the grease food-grade standard is improved.

The invention provides a functional feed additive for improving the quality of tilapia mossambica. The functional feed additive comprises the following raw materials: hydrolyzed fish meal, fermented soybean meal, insect protein powder, fish soluble, callicarpa nudiflora powder, fish oil, polysaccharide, an intestine regulating agent and vitamins. The preparation method of the hydrolyzed fish meal comprises the following steps: crushing fish, adding a Na2CO3 solution for rinsing, performing high-temperature and high-pressure squeezing for degreasing, adding a mixed enzyme solution for enzymolysis, performing enzyme deactivation for 2-3 times, performing centrifugal separation, concentrating, drying, crushing and sieving. Wherein the polysaccharide is fucoidin and trametes robiniophila polysaccharide, and the intestine regulating agent is lactobacillus rhamnosus powder and lactobacillus kefir powder. The feed additive is balanced in formula nutrition, improves the quality of tilapia mossambica, reduces intestinal diseases of tilapia mossambica, and has application value.

A process and product obtainable by acid hydrolysis of insects, for example from cricket powder. As one application, an acid hydrolysis product made from powdered crickets may be used as a seasoning sauce resembling soy sauce. The insect powder is hydrolyzed with acid, followed by neutralization, and optionally clarification. In comparison to the typical production of soy sauce, the acid hydrolysis procedure uses insect protein as the main substrate or even the sole substrate. The flavor of soy sauce-like products made from acid-hydrolyzed cricket protein results from a complex combination of compounds generated by the reactions.

The application discloses a straw ant cultivation method, which comprises the following steps: plot selection, land preparation, ant nest composite planting, straw feeding and mushroom management; the straw ant cultivation method is based on the biological ecology principle of ant foraging behavior, aims at creating suitable microorganisms, temperature and humidity microenvironment under different environments, and providing straw food to increase the foraging efficiency of ants, so as to increase the efficiency of straw decomposition of the ant nest system; in addition, the straw ant cultivation method can help to relieve the agricultural environmental problems of straw recycling, increase the yield of wild chicken conk and ant insect protein, and create economic value with high added value.

The invention relates to a kitchen garbage recycling method which comprises the following steps: pretreating kitchen garbage to obtain a kitchen garbage oil phase, a kitchen garbage liquid phase and a kitchen garbage solid phase; placing the eggs on the surface of wheat bran for hatching to obtain larvae; inoculating the larvae on the surface of the kitchen waste solid phase for breeding, and adding solid residues to obtain a solid mixture; and separating the solid mixture to obtain adults and fecula. The kitchen garbage is fed by insects and is converted into various high-value products: the insect protein can replace conventional feeds such as fish meal and soybean meal, so that the pressure of feed resources is relieved; the insect fat can be used for producing biodiesel or cosmetic raw materials; the insect sand is a high-quality organic fertilizer and can effectively improve the soil fertility. And finally, efficient conversion and sustainable cyclic utilization of the kitchen garbage are realized.

The present invention provides a preparation method for 3D printing snacks using insect protein powder. It comprises the following steps: mixing defatted insect protein powder with a heat-stable thickening agent; adding a composite liquid containing glycerol and sodium citrate, and shearing to form a gel-like paste; forming an initial thermoregulatory structure through low-temperature curing; using a temperature-controlled food 3D printing device for layer-by-layer deposition printing; after printing, allowing it to stand at room temperature to achieve self-stabilization of the structure.

The invention discloses a method for preparing insect protein peptide by utilizing bacillus velezensis JAAS-22. The method comprises the following steps: (1) performing amplification culture on a strain of the bacillus velezensis JAAS-22 to obtain a bacillus velezensis JAAS-22 fermentation culture solution; (2) homogenizing insect larvae in a colloid mill to obtain insect pulp; (3) uniformly mixing the insect pulp with the bacillus velezensis JAAS-22 fermentation culture solution, and carrying out enzymolysis fermentation to obtain an insect pulp enzymolysis fermentation product; and (4) dialyzing the enzymatic hydrolysis fermentation product of the insect pulp to obtain the insect protein peptide with the molecular weight less than or equal to 30 kDa. According to the method, the bioactive peptide with stable and good antibacterial activity can be efficiently prepared.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The present application provides a camel-derived anti-insect protein and its coding gene and application, and belongs to the field of genetic engineering and biological control; the nucleotide and amino acid sequences of the anti-insect protein are shown in SEQ IN NO. 1 and SEQ IN NO. 2 respectively, the anti-insect protein is a single-domain antibody with insecticidal activity screened from a camel-derived nanobody library, has small molecular weight, is simple to prepare, and is derived from a llama, is used for agricultural pest control, and has important scientific and practical significance for expanding new insecticidal gene resources.

The invention provides aquarium fish feed for gullfish and a preparation method of the aquarium fish feed, and relates to the technical field of aquatic product feeding. The aquarium fish feed is prepared from hermetia illucens larva powder, white fish meal, haematococcus pluvialis wall-broken powder, lycium barbarum polysaccharide extract, squid viscera hydrolysate, compound vitamin premix, mineral premix, yeast beta-glucan, Chinese herbal medicine extract, compound probiotics and sodium alginate. According to the invention, the defects in the prior art are overcome, the insect protein powder is used for replacing fish meal / cattle heart, natural color enhancing components are added, and probiotics and Chinese herbal medicines are combined for compounding, so that the synergistic effect of nutrition, environmental protection and functionality is realized, the growth of the symphysodon is effectively promoted, and water pollution is reduced.

This disclosure provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences encoding insecticidal proteins. Specifically, the nucleic acid sequences can be used to prepare plants and microorganisms with insecticidal activity. Therefore, this disclosure provides transformed bacteria, plants, plant cells, plant tissues, and seeds. The compositions are insecticidal nucleic acids and proteins of bacterial strains. The sequences can be used to construct expression vectors for subsequent transformation into organisms of interest, including plants, and as probes for isolating other homologous (or partially homologous) genes. The insecticidal proteins can be used to control, kill, or inhibit the growth of the following pest populations: Lepidoptera, Coleopteran, Dipteran, fungi, Hemipteran, and nematodes, and can also be used to produce compositions with insecticidal activity.

The utility model belongs to the technical field of supercritical extraction, and particularly relates to a supercritical extraction device suitable for insect protein production, which comprises an extraction kettle, the top of the extraction kettle is fixedly connected with a servo motor; the transmission end of the servo motor is fixedly connected with a rotating shaft; the bottom end of the rotating shaft is rotationally connected to the bottom of the inner side wall of the extraction kettle; the outer side wall of the rotating shaft is rotationally connected with a first rotating frame. The side wall, close to the extraction kettle, of the first rotating frame is slidably connected with a fixed plate; a plurality of sections of fixing plates are arranged on the side wall of the first rotating frame; according to the step, a first rotating frame, a fixing plate, a brush and a spring are arranged to form an extraction kettle internal cleaning structure, the function of cleaning the inner side wall of the extraction kettle is achieved, the problem that the extraction kettle needs to be opened and other tedious steps are needed when the extraction kettle is cleaned is solved, the cleanliness of the inner side wall of the extraction kettle is improved, residual materials attached to the inner side wall of the extraction kettle are reduced, and the production efficiency is improved. And the cleaning operation steps are simplified.

Compositions and methods for controlling corn plant pests are disclosed. In particular, novel insecticidal proteins that are toxic against coleopteran and / or lepidopteran insect pests are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Also disclosed are methods of making the insecticidal proteins and methods of using the insecticidal proteins as well as nucleic acids encoding the insecticidal proteins of the invention, e.g., to confer protection from insect damage in transgenic maize plants.

The invention discloses a hermetia illucens breeding feed based on waste mushroom sticks. The hermetia illucens breeding feed comprises mushroom stick residues and eutrophic balls which are uniformly dispersed, 0.2-2% by mass of a compound microbial agent is uniformly added into the mushroom stick residues; the compound microbial agent is prepared from 25 to 35 parts of bacillus subtilis, 15 to 25 parts of lactic acid bacteria, 13 to 17 parts of actinomycetes, 13 to 17 parts of saccharomycetes, 8 to 12 parts of photosynthetic bacteria and 8 to 12 parts of bacillus aceticus; the eutrophic ball comprises a ball core and a ball shell, the ball core comprises mushroom stick powder, bacillus aceticus, bacillus subtilis, a microelement additive, pregelatinized starch and carboxymethyl cellulose which are uniformly mixed, and the ball shell comprises starch and brown sugar which are uniformly mixed. The invention further discloses a preparation method of the hermetia illucens culture based on the waste mushroom sticks, a hermetia illucens culture method and a fecula treatment method. The black soldier fly breeding feed based on the waste mushroom sticks is high in biotransformation rate; the closed-loop ecological cycle of the mushroom stick residues, the insect protein and the organic fertilizer is realized.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming a plant, plant tissue, plant part, plant cell, and plant seed with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The invention discloses a construction method of an animal model for insect protein food allergy. The constructed animal model can be used for screening potential substances for resisting insect protein allergy. The food allergy animal model constructed by adopting the insect protein is applied to research on sensitization of edible insects serving as a novel protein source, and a research model is provided for clinical research on insect protein allergy or processing methods and substance screening for resisting insect allergy.

Novel insecticidal polypeptides that are active against Coleopteran pests are disclosed. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. The nucleotide sequences encoding the insecticidal polypeptides can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. Methods of making the insecticidal proteins and methods of using the insecticidal proteins, for example in transgenic plants to confer protection from insect damage are also disclosed.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of infants. The culture medium contains a yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, breast milk oligosaccharide, dietary fiber, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, a plant extract mixture, a vitamin mixed solution and the like. By means of the bionic colon reactor, the real intestinal environment of domestic infants is well simulated in vitro by referring to real parameters in the infants, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the infants can be constructed. Accurate diagnosis and personalized treatment of the intestinal microecology of the infants are facilitated, the health state of the infants can be better evaluated, and preventive measures can be better formulated.

The application discloses an insect protein low-temperature extraction device and relates to the technical field of protein extraction equipment. The application comprises an operation box, the top of the operation box is fixedly connected with a feeding inlet, a filter screen is arranged below the feeding inlet, the filter screen is rotatably installed in the inside of a filter box, the lower portion of the filter screen is in contact with the protruding end of a cam, the cam is fixedly installed on a connecting shaft, the connecting shaft is fixedly connected with the output end of a driving motor, one end of the connecting shaft is connected with a first gear through a transmission mechanism, the transmission mechanism is used for controlling the rotation of the first gear, and the first gear is engaged with a second gear. The rotatable and inclined filter screen is beneficial to the sliding and discharge of impurities, the cam promotes the vibration of the filter screen to prevent blockage, the cleaning brush is used for cleaning the inner wall of the filter box and extruding residual materials of the filter screen when the cleaning brush rotates, and the reciprocating threaded rod is used for driving a cleaning scraper to clean the inner wall of the operation box. The designs are coordinated, and the extraction rate of insect protein is effectively improved.

The utility model relates to the technical field of protein production, in particular to an insect protein grinding device which comprises a grinding tank and a grinding roller, a grinding groove is formed in the grinding tank, and the outer side face of the grinding groove is connected with the inner side wall of the grinding tank; a gap between the upper end of the grinding roller and the inner side surface of the grinding groove is gradually reduced from top to bottom, and the lower end of the grinding roller is inserted into the bottom wall of the grinding groove to form clearance fit; and filtering holes are formed in the bottom wall of the grinding groove corresponding to the lower end surfaces of the grinding rollers. The utility model can solve the problem that the fine grinding efficiency of the existing protein production device is not high enough.

The invention discloses a silkworm pupa protein functional fermentation base material and application thereof in fermented meat products, and belongs to the technical field of food processing. The fermentation base material comprises a specific microbial fermentation agent, fresh silkworm chrysalis slurry and meat paste, and a stable protein co-gel network is formed through low-temperature fermentation. The leavening agent comprises lactobacillus sake and staphylococcus calvus or lactobacillus plantarum, and the viable count is larger than or equal to 1.0 * 10 < 8 > CFU / g. The addition amount of the fresh silkworm chrysalis slurry is 10-30%, the fermentation temperature is 4-10 DEG C, the fermentation time is 3-10 days, and the pH value is reduced to 5.2-5.5. The fermented meat product prepared from the base material has excellent flavor, texture and water-retaining property, and the residual quantity of nitrite is remarkably reduced. The invention provides a new technical scheme for high-valued application of the insect protein in meat products.