32 results about "Insect Proteins" patented technology

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

The invention belongs to the technical field of solenopsis invicta prevention and control, and particularly relates to solenopsis invicta food bait as well as a preparation method and application thereof. The invention provides red imported fire ant food bait. The red imported fire ant food bait is prepared from energy main materials and auxiliary materials, the energy main material consists of the following components in percentage by weight: 50-70% of insect protein powder, 5-20% of corn flour, 9-11% of peanut oil, 9-11% of fish meal and 9-11% of cane sugar. The invention also provides an attractant matched with the red imported fire ant food bait. According to the invention, the efficiency of finding bait by the red imported fire ants can be improved, meanwhile, the recruitment behaviors of the worker ants are further increased, more worker ants are lured, and the comprehensive trapping effect is good.

The present invention provides methods for controlling Ostrinia furnacalis and protecting crops, in particular corn, from economic damage caused by Ostrinia furnacalis. The invention further relates to the use of plants stably transformed with nucleic acid molecules encoding the Cry proteins of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Ostrinia furnacalis.

The present invention provides a method for controlling Ostrinia furnacalis (Ostrinia furnacalis) and protecting a crop, in particular corn, from economic damage caused by the Ostrinia furnacalis (Ostrinia furnacalis). The invention further relates to the use of a plant stably transformed with a nucleic acid molecule encoding a Cry protein of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Asiatic Ostrinia furnacalis.

A process and product obtainable by acid hydrolysis of insects, for example from cricket powder. As one application, an acid hydrolysis product made from powdered crickets may be used as a seasoning sauce resembling soy sauce. The insect powder is hydrolyzed with acid, followed by neutralization, and optionally clarification. In comparison to the typical production of soy sauce, the acid hydrolysis procedure uses insect protein as the main substrate or even the sole substrate. The flavor of soy sauce-like products made from acid-hydrolyzed cricket protein results from a complex combination of compounds generated by the reactions.

The application discloses a straw ant cultivation method, which comprises the following steps: plot selection, land preparation, ant nest composite planting, straw feeding and mushroom management; the straw ant cultivation method is based on the biological ecology principle of ant foraging behavior, aims at creating suitable microorganisms, temperature and humidity microenvironment under different environments, and providing straw food to increase the foraging efficiency of ants, so as to increase the efficiency of straw decomposition of the ant nest system; in addition, the straw ant cultivation method can help to relieve the agricultural environmental problems of straw recycling, increase the yield of wild chicken conk and ant insect protein, and create economic value with high added value.

The invention relates to a kitchen garbage recycling method which comprises the following steps: pretreating kitchen garbage to obtain a kitchen garbage oil phase, a kitchen garbage liquid phase and a kitchen garbage solid phase; placing the eggs on the surface of wheat bran for hatching to obtain larvae; inoculating the larvae on the surface of the kitchen waste solid phase for breeding, and adding solid residues to obtain a solid mixture; and separating the solid mixture to obtain adults and fecula. The kitchen garbage is fed by insects and is converted into various high-value products: the insect protein can replace conventional feeds such as fish meal and soybean meal, so that the pressure of feed resources is relieved; the insect fat can be used for producing biodiesel or cosmetic raw materials; the insect sand is a high-quality organic fertilizer and can effectively improve the soil fertility. And finally, efficient conversion and sustainable cyclic utilization of the kitchen garbage are realized.

The present invention provides a preparation method for 3D printing snacks using insect protein powder. It comprises the following steps: mixing defatted insect protein powder with a heat-stable thickening agent; adding a composite liquid containing glycerol and sodium citrate, and shearing to form a gel-like paste; forming an initial thermoregulatory structure through low-temperature curing; using a temperature-controlled food 3D printing device for layer-by-layer deposition printing; after printing, allowing it to stand at room temperature to achieve self-stabilization of the structure.

The invention discloses a method for preparing insect protein peptide by utilizing bacillus velezensis JAAS-22. The method comprises the following steps: (1) performing amplification culture on a strain of the bacillus velezensis JAAS-22 to obtain a bacillus velezensis JAAS-22 fermentation culture solution; (2) homogenizing insect larvae in a colloid mill to obtain insect pulp; (3) uniformly mixing the insect pulp with the bacillus velezensis JAAS-22 fermentation culture solution, and carrying out enzymolysis fermentation to obtain an insect pulp enzymolysis fermentation product; and (4) dialyzing the enzymatic hydrolysis fermentation product of the insect pulp to obtain the insect protein peptide with the molecular weight less than or equal to 30 kDa. According to the method, the bioactive peptide with stable and good antibacterial activity can be efficiently prepared.

The present application provides a camel-derived anti-insect protein and its coding gene and application, and belongs to the field of genetic engineering and biological control; the nucleotide and amino acid sequences of the anti-insect protein are shown in SEQ IN NO. 1 and SEQ IN NO. 2 respectively, the anti-insect protein is a single-domain antibody with insecticidal activity screened from a camel-derived nanobody library, has small molecular weight, is simple to prepare, and is derived from a llama, is used for agricultural pest control, and has important scientific and practical significance for expanding new insecticidal gene resources.

This disclosure provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences encoding insecticidal proteins. Specifically, the nucleic acid sequences can be used to prepare plants and microorganisms with insecticidal activity. Therefore, this disclosure provides transformed bacteria, plants, plant cells, plant tissues, and seeds. The compositions are insecticidal nucleic acids and proteins of bacterial strains. The sequences can be used to construct expression vectors for subsequent transformation into organisms of interest, including plants, and as probes for isolating other homologous (or partially homologous) genes. The insecticidal proteins can be used to control, kill, or inhibit the growth of the following pest populations: Lepidoptera, Coleopteran, Dipteran, fungi, Hemipteran, and nematodes, and can also be used to produce compositions with insecticidal activity.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming a plant, plant tissue, plant part, plant cell, and plant seed with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

Novel insecticidal polypeptides that are active against Coleopteran pests are disclosed. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. The nucleotide sequences encoding the insecticidal polypeptides can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. Methods of making the insecticidal proteins and methods of using the insecticidal proteins, for example in transgenic plants to confer protection from insect damage are also disclosed.

The application discloses an insect protein low-temperature extraction device and relates to the technical field of protein extraction equipment. The application comprises an operation box, the top of the operation box is fixedly connected with a feeding inlet, a filter screen is arranged below the feeding inlet, the filter screen is rotatably installed in the inside of a filter box, the lower portion of the filter screen is in contact with the protruding end of a cam, the cam is fixedly installed on a connecting shaft, the connecting shaft is fixedly connected with the output end of a driving motor, one end of the connecting shaft is connected with a first gear through a transmission mechanism, the transmission mechanism is used for controlling the rotation of the first gear, and the first gear is engaged with a second gear. The rotatable and inclined filter screen is beneficial to the sliding and discharge of impurities, the cam promotes the vibration of the filter screen to prevent blockage, the cleaning brush is used for cleaning the inner wall of the filter box and extruding residual materials of the filter screen when the cleaning brush rotates, and the reciprocating threaded rod is used for driving a cleaning scraper to clean the inner wall of the operation box. The designs are coordinated, and the extraction rate of insect protein is effectively improved.

The invention discloses a silkworm pupa protein functional fermentation base material and application thereof in fermented meat products, and belongs to the technical field of food processing. The fermentation base material comprises a specific microbial fermentation agent, fresh silkworm chrysalis slurry and meat paste, and a stable protein co-gel network is formed through low-temperature fermentation. The leavening agent comprises lactobacillus sake and staphylococcus calvus or lactobacillus plantarum, and the viable count is larger than or equal to 1.0 * 10 < 8 > CFU / g. The addition amount of the fresh silkworm chrysalis slurry is 10-30%, the fermentation temperature is 4-10 DEG C, the fermentation time is 3-10 days, and the pH value is reduced to 5.2-5.5. The fermented meat product prepared from the base material has excellent flavor, texture and water-retaining property, and the residual quantity of nitrite is remarkably reduced. The invention provides a new technical scheme for high-valued application of the insect protein in meat products.

The present invention provides methods for controlling Spodoptera litura and protecting crops, in particular maize, from economic damage caused by Spodoptera litura. The invention further relates to the use of plants stably transformed with nucleic acid molecules encoding the Cry proteins of the invention, alone or in combination with other insecticidal proteins, for controlling or combating Spodoptera litura.

The invention relates to a storage hardening resistant rice locust powder-whey protein compound protein bar and a preparation method thereof, rice locust powder prepared from Chinese rice locust is compounded with whey protein concentrate to serve as a main protein component of the protein bar, the protein bar is applied to a low-sugar or high-sugar system, and protein food is prepared; based on the characteristics, the rice locust powder-whey protein compound protein bar can be prepared, and texture hardening and browning caused by Maillard reaction in the storage process of the protein bar are remarkably inhibited on the basis of ensuring relatively high protein supply and flexibly controlling the sugar content. A low-water-activity system constructed by the rice locust powder-whey protein compound and fructo-oligosaccharide or glucose syrup realizes cold processing forming, and protein excessive denaturation and lipid oxidation caused by high-temperature processing are avoided; the preparation process is simple in flow and suitable for continuous production; the method also provides a new path for structured utilization of the insect protein in functional food.

The present application belongs to the field of red imported fire ant prevention and control technology, and particularly relates to a red imported fire ant food bait, a preparation method and application thereof. The present application provides a red imported fire ant food bait, which is composed of energy main material and auxiliary material; the energy main material is composed of the following components with the weight content: insect protein powder 50-70%, corn flour 5-20%, peanut oil 9-11%, fish meal 9-11% and sucrose 9-11%. The present application also simultaneously provides an attractant matched with the above-mentioned red imported fire ant food bait. The present application can increase the discovery efficiency of foraging workers of red imported fire ants on the bait, further increase the recruitment behavior of the workers, attract more workers, and has good comprehensive trapping effect.

According to the breeding feed with the insect protein source as the raw material and the preparation method of the breeding feed, hermetia illucens are used as the insect protein source, pathogens are rapidly inactivated through sequential coupling of high-temperature composting and anaerobic digestion, protein denaturation caused by traditional heat treatment is avoided, the activity of anaerobic bacteria is maintained through composting waste heat, and the breeding efficiency is improved. The hermetia illucens are specifically decomposed into hard shells, and the digestibility of poultry to insect powder is remarkably improved. The prepared breeding feed has multiple effects of improving immunity of bred animals, resisting bacteria and diminishing inflammation, promoting growth, improving meat quality and flavor and the like. According to the preparation method, sterilization and insect shell degradation are completed through the same reaction system, the defects of high energy consumption and long period of a traditional technology are overcome, meanwhile, mulberry leaves, wormwood and the like are used as swelling agents, the air permeability of a pile body is improved, the porosity is improved, aerobic fermentation is accelerated, synergistic bacteriostasis and digestion promotion are achieved, popularization and production are easy, and the green production trend of livestock and poultry feed is met.

The present invention provides a method for controlling spodoptera litura and for protecting a crop, in particular corn, from economic damage caused by spodoptera litura. The present invention provides a method for controlling spodoptera litura and for protecting a crop, in particular corn, from economic damage caused by spodoptera litura. The invention further relates to the use of a plant stably transformed with a nucleic acid molecule encoding a Cry protein of the invention, alone or in combination with other insecticidal proteins, for controlling or combating spodoptera litura.

This disclosure relates to nucleic acid sequences that confer expression of insecticidal proteins when introduced into cells, and related compositions and methods of use thereof. In some aspects, this disclosure provides plants containing such nucleic acid sequences.

The invention provides nucleic acids, polypeptides, transgenic plants, compositions and methods for conferring pesticidal activity (e.g., insecticidal activity) to bacteria, plants, plant cells, tissues and seeds. Nucleic acids encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or insecticidal proteins or in combination with an appropriate agricultural carrier can be used to control an insect pest in various environments.

The invention provides a method for preparing insect protein from kitchen garbage, application of the method and the insect protein, and relates to the technical field of solid waste resource utilization, and the method comprises the following steps: pretreating the kitchen garbage, and then converting the kitchen garbage into protein through insects to obtain the insect protein; the pretreatment comprises the following steps: carrying out oil-water separation on the crushed kitchen garbage, carrying out enzymolysis treatment by virtue of a compound enzyme, and fermenting by virtue of a compound microbial agent, so as to obtain pretreated kitchen garbage; the compound enzyme comprises alpha-amylase, acid protease and cellulase; the compound microbial agent comprises bacillus subtilis, lactic acid bacteria and saccharomycetes. The technical problem that in the prior art, the insect protein yield and quality are low is solved, and the technical effects of being efficient, environmentally friendly and capable of effectively improving the insect protein yield and quality are achieved.

The present invention provides methods of controlling the Lepidoptera, e.g., the Chlortridae family and protecting crops, particularly corn, from economic damage of the Lepidoptera, e.g., the Chlortridae family. The invention further relates to the use of a plant stably transformed with a nucleic acid molecule encoding a Cry protein of the invention, alone or in combination with other insecticidal proteins, for the control or combat of Lepidoptera, such as the Choridae family. The present invention provides a method for inhibiting the growth of or killing a pest, a method for controlling a population of a pest, a method for protecting a plant from a pest, wherein the pest comprises a pest of the family Pseudomonas sp., in particular Sorghum prolocrocis.

This invention relates to the field of insect protein processing and discloses a method for preparing highly soluble black soldier fly larvae protein using trypsin-assisted methods. The method involves freeze-drying, pulverizing, and defatting with petroleum ether of black soldier fly larvae. Trypsin is then added at a mass ratio of enzyme to defatted insect powder of 1:100. Enzymatic hydrolysis is performed for 1 h at pH 7.8 and 37°C in a buffer system of 25 mM ammonium bicarbonate and 2.5 mM calcium chloride. Following this, alkaline extraction is carried out for 2 h at a material-to-liquid ratio of 1:25, pH 12.8, and 53°C. The resulting protein is then subjected to centrifugation, precipitation at pH 4.8 isoelectric point, reconstitution and neutralization, dialysis, concentration, and freeze-drying to obtain the black soldier fly larvae protein isolate. The obtained protein has a crude protein content of not less than 90 wt% and a solubility of not less than 70% at pH 7. It is suitable for rehydrated dispersion systems, liquid protein formulation systems, and oil / water emulsion systems.

The invention discloses a pellet feed for laboratory mice and a preparation method of the pellet feed. The feed consists of the following raw materials in percentage by weight: 42-50% of corn, 8-12% of fermented soybean meal, 4-8% of insect protein (hermetia illucens larva powder), 2-4% of maltodextrin, 1-2% of inulin, 0.5-2% of inactivated enterococcus faecalis, 1.5-3% of a vitamin premix, 3-5% of a mineral premix, 0.3-0.8% of xylooligosaccharide, 2-4% of vegetable oil, 1-3% of cellulose and the balance of auxiliary materials. The method has the advantages that heavy metal and micro-plastic pollution are avoided, allergens are removed, and the survival rate of laboratory mice is increased by 20%; active ingredients of the inactivated enterococcus faecalis are reserved through a spraying process, and the number of beneficial bacteria in the intestinal tract is increased by 25-35% compared with a contrast; the feed conversion rate is increased by 21-32%, the weight gain is stable, and the fluctuation of experimental data is reduced.

The application discloses cricket-derived multifunctional small-molecule bioactive peptides, a preparation method of the peptides and application of the peptides in medicines, functional foods and health products. The amino acid sequence of the multifunctional small-molecule bioactive peptides is NYGGY, and the peptides can be obtained by enzymatic hydrolysis of cricket protein or solid-phase chemical synthesis. Activity determination of the cricket protein hydrolysate shows that the cricket protein hydrolysate has good antioxidant, blood pressure-lowering and blood glucose-lowering activities. Further mass spectrometry detection proves that the cricket protein hydrolysate contains the active peptide sequence of the application, and through molecular docking simulation prediction and in-vitro activity verification, the two peptide segments and the related targets all show strong binding capacity. The multifunctional small-molecule bioactive peptides of the application are derived from edible insect protein, are safe and natural in raw materials, meet the green and sustainable development concept, are easy to be prepared on a large scale, and have good industrial transformation potential.

Disclosed are compositions and methods for controlling insect pests. In particular, novel insect inhibitory proteins comprising two different components, both of which are necessary for biological activity against at least a coleopteran insect, are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Methods of making the insecticidal proteins, and methods of using the insecticidal proteins and nucleic acids encoding the insecticidal proteins of the invention, e.g., to confer protection from insect damage in transgenic plants, are also disclosed.

The invention is entitled "Insecticidal Proteins and Methods of Use Thereof." The invention provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences that encode insecticidal proteins. In particular, the nucleic acid sequences are useful for making plants and microorganisms that have insecticidal activity. Thus, the invention provides transformed bacteria, plants, plant cells, plant tissues, and seeds. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences are useful for constructing expression vectors for subsequent transformation into organisms of interest, including plants, as probes for isolating other homologous (or partially homologous) genes. The pesticidal proteins are useful for controlling, inhibiting the growth of, or killing Lepidoptera, Coleoptera, Diptera, fungal, Hemipteran, and nematode pest populations, as well as for producing compositions having insecticidal activity.

To provide an emulsion composition using a suitable raw material from the viewpoint of response to milk allergy and environmental response, and having excellent emulsion stability.SOLUTION: An oil-in-water emulsion composition according to an example comprises water, an oil, and solid particles, wherein the solid particles are present at the interface between the water and the oil, at least some of the solid particles are insect proteins, and the pH of an aqueous phase or an aqueous dispersion containing the solid particles is 7.5 or less. In another example, the surface tensions of the aqueous phases may be 32mN / m or more.SELECTED DRAWING: None