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Intelligent rice blast germ spore separation and preservation system and method

A technology for spore separation and rice blast, which is applied in the methods of using spores, seed and rhizome treatment, biochemical equipment and methods, etc., which can solve the problem of rapid genetic variation of races, limited planting years and suitable areas of disease-resistant rice varieties and other problems to achieve the effect of improving the effectiveness of preservation, improving the success rate of separation, and improving the success rate

Pending Publication Date: 2021-02-02
INST OF CROP SCI NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The significance of solving the above problems and defects is: rice blast fungus populations are complex and diverse, race genetic variation is fast, and the planting years and suitable areas of disease-resistant rice varieties are limited

Method used

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  • Intelligent rice blast germ spore separation and preservation system and method
  • Intelligent rice blast germ spore separation and preservation system and method
  • Intelligent rice blast germ spore separation and preservation system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] The intelligent method for separating and preserving the spores of rice blast fungus provided by the invention comprises:

[0107] 1) Bacteria sampling and storage

[0108] Collect samples from diseased plants. Specimens including diseased panicle necks and diseased panicle nodes are uniformly numbered, dried, placed in airtight containers, and stored in a 4°C refrigerator for later use.

[0109] 2) Moisturizing culture

[0110] Place the rice blast specimen in sterile water containing 2ml of the antibiotic rifampicin (diluted with 25mg / ml alcohol), soak for 10h to 24h, take it out and place it on the sterilized specimen holder, put two sheets soaked with antibiotics under the specimen holder Petri dish with sterile water filter paper, moist culture for 48h ~ 72h.

[0111] 3) Isolation of rice blast spores

[0112] The specimens cultivated by moisture were shaken off by the shake-off method, and the spores were shaken off into the sterilized water agar medium, and t...

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Abstract

The invention belongs to the technical field of rice blast germ spore separation, and discloses an intelligent rice blast germ spore separation and preservation system and method for setting a diseasenursery and sampling and preserving germs. The method comprises the following steps of putting a rice blast specimen into sterile water containing 2ml of antibiotic rifampicin, soaking for 10-24 hours, taking out, and putting on a sterilized specimen support; putting two culture dishes soaked with antibiotic sterile water filter paper into the specimen support, and carrying out moisturizing culture for 48-72 hours; shaking off spores of the specimen subjected to moisturizing culture into a sterilized water agar culture medium by using a shaking-off method; putting the spore-containing water agar culture dish into a 26 DEG C constant-temperature incubator, and culturing for 16-20 hours; preparing an inoculum; carrying out differential host cultivation; and inoculating the inoculum on the cultured host. The problems that in the prior art, the rice blast germ spore separation and preservation intelligent effect is poor, and practicability is lacked are solved.

Description

technical field [0001] The invention belongs to the technical field of separation of rice blast fungus spores, and in particular relates to an intelligent system and method for separating and preserving rice blast fungus spores. Background technique [0002] At present, rice blast is a disease of rice caused by the pyrosporium fungus, also known as rice fever, fire blast, and knocking blast. The fungus infects leaves, stalks, ear necks, spikelets and grains. Depending on the time of injury and the site of infection, it can cause seedling blast, leaf blast, knot blast, panicle blast, and grain blast respectively. Rice blast is an important fungal disease, and it is one of the important diseases that affect the high and stable yield of rice. In recent years, with the promotion of disease-resistant varieties, the disease has been effectively controlled, but with the extension of the planting period of the disease-resistant varieties, their resistance has been overcome, result...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12N1/04C12M1/00C12M1/22A01G9/029A01C1/08A01C1/00C12R1/645
CPCC12N3/00C12N1/14C12N1/04C12M23/10C12M33/00A01G9/0293A01C1/08A01C1/00
Inventor 史延丽陈东升白小军刘炜杨生龙强爱玲王彩芬黄新玲沙蓉蒲丽丽王玲李玉红
Owner INST OF CROP SCI NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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