Oligopeptide used for inhibiting fungal biofilm and application of oligopeptide

Abstract

The invention belongs to the technical field of antifungal drugs, and discloses an oligopeptide used for inhibiting a fungal biofilm and application of the oligopeptide. The oligopeptide used for inhibiting the fungal biofilm is a bacterial secretion product, and is used for inhibiting formation of the fungal biofilm; a method for applying the oligopeptide to inhibit the fungal biofilm includes the following steps: artificially synthesizing bacterial oligopeptide XIP; collecting saliva-coating a biofilm culture plate; inoculating culture plate with fungi and adding the XIP oligopeptide to culture the biofilm; and quantifying the biomass of the fungal biofilm by a crystal violet staining method. According to the invention, the XIP oligopeptide secreted by streptococcus mutans is artificially synthesized; the XIP oligopeptide is added in the candida albicans biofilm forming process; and the inhibition effect of the XIP oligopeptide on candida albicans biofilm formation is quantitativelydetected by using the crystal violet staining method after culture is performed for 24h. The oligopeptide generated by bacteria acts on the candida albicans biofilm, so that the formation of the candida albicans biofilm is effectively inhibited.

Description

technical field [0001] The invention belongs to the technical field of antifungal drugs, in particular to an oligopeptide for inhibiting fungal biofilm and its application. Background technique [0002] Bacterial drug resistance is well known, but fungal drug resistance has not received enough attention. The emergence of antifungal drug resistance has brought great challenges to the clinical treatment of invasive fungal infections. Currently, only a few classes of antifungal drugs are clinically available, and the emergence of drug resistance means that many common fungal infections become difficult to treat. In addition, the development time of antifungal drugs is long, the investment is huge, and the price after marketing is generally expensive, which brings serious economic burden to patients and is also limited to a certain extent in clinical use. Existing drugs cannot fully meet the needs of clinical treatment, and reports of drug resistance to drug treatment are grad...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems existing in the prior art, on the basis of the research on the mechanism of Streptococcus mutans antagonizing Candida albicans, the present invention provides a new oligopeptide for inhibiting fungal biofilm and its application, aiming to help solve the existing problems. There is growing antifungal resistance and lack of new effective drug candidates

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