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Double-layer Raman molecular marked silicon-core silver-shell composite nano label, preparation method and immunochromatography application

A technique of Raman molecular and immunochromatography, which is applied in the analysis of materials, Raman scattering, material excitation analysis, etc., can solve the problems of only qualitative or semi-quantitative detection, low detection sensitivity, etc., and achieves convenient and highly sensitive preparation methods Good detection and monodispersity

Pending Publication Date: 2021-02-05
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional immunochromatography technology relying on gold / silver colloid as the substrate has the defects of low detection sensitivity and only qualitative or semi-quantitative detection.

Method used

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  • Double-layer Raman molecular marked silicon-core silver-shell composite nano label, preparation method and immunochromatography application
  • Double-layer Raman molecular marked silicon-core silver-shell composite nano label, preparation method and immunochromatography application
  • Double-layer Raman molecular marked silicon-core silver-shell composite nano label, preparation method and immunochromatography application

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The double-layer Raman molecule-labeled silicon-core-silver-shell composite nano-label prepared by the present invention includes SiO 2 Core, PEI self-assembly layer, shell formed by reduction of silver ions, and double-layer DTNB molecules. The first layer of Raman signal molecule DTNB is firmly bound to SiO through Au-S bonds. 2 @Au-seed surface, the second layer of DTNB is tightly combined with the Ag shell through the Ag-S bond. The synthesized silicon-core-silver-shell composite nanolabel has good dispersion and super SERS performance.

[0050] A kind of preparation method of the silicon-core-silver-shell composite nano-label of double-layer Raman molecular labeling of the above-mentioned present embodiment, such as figure 1 shown, including the following steps:

[0051] (1) Preparation of SiO 2 @PEI nanoparticles:

[0052] Take 1mL SiO 2 The particle solution was dissolved in 100 mL deionized water, and then reacted with freshly prepared PEI solution (1 mg / m...

Embodiment 2

[0064] The surface of the double-layer Raman molecule-labeled silicon-core-silver-shell composite nanolabel proposed by the present invention is a carboxylated silver shell, and the surface has a large number of free carboxyl groups, which can be used for the coupling of the new coronavirus recombinant antigen S protein, which can be realized very easily. Surface functionalization of nanomaterials. The steps of S protein modification on the surface of the double-layer Raman molecularly labeled silicon-core-silver-shell composite nano-label of the present invention are as follows Figure 4 shown, including the following steps:

[0065] Take 500 μL of double-layer DTNB-modified SiO 2 @Ag solution, add 500 μL MES buffer (100 mM, pH5.5) containing 50 μL carbodiimide solution (0.01M) and 10 μL N-hydroxysuccinimide solution (0.1M) to activate SiO by ultrasonic reaction for 15 minutes 2 Carboxyl groups on the surface of @Ag-DTNB. Subsequently, it was incubated with 30 μg / mL of the...

Embodiment 3

[0067] The double-layer Raman molecule-labeled silicon-core-silver-shell composite nano-label proposed by the present invention is used as a high-performance SERS label for the detection of an immunochromatography system. In this example, the novel coronavirus recombinant antigen S protein-modified double-layer Raman molecule-labeled silicon-core-silver-shell composite nano-label is combined with an immunochromatographic system to simultaneously detect different concentrations of IgM and IgG in human positive serum. Figure 5 It is an experimental flow chart for the rapid detection of IgM and IgG in human serum by using the double-layer Raman molecule-labeled silicon-core-silver-shell composite nano-tag as a SERS tag in conjunction with an immunochromatography system as shown in this example. Figure 6 It is the optimization diagram of the preliminary experiment for the detection of human IgM and IgG in serum by combining the double-layer Raman molecule-labeled silicon-core-sil...

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Abstract

The invention discloses a double-layer Raman molecular marked silicon-core silver-shell composite nano label, a preparation method and immunochromatography application. The double-layer Raman molecular marked silicon-core silver-shell composite nano label sequentially comprises a SiO2 inner core, a PEI layer, a colloidal gold layer, a first DTNB layer, a nano Ag layer and a second DTNB layer frominside to outside, wherein the surface of the second DTNB layer is coupled with novel coronavirus recombinant antigen S proteins. The invention further discloses a preparation method of the compositenano-label and application of the composite nano-label in immunochromatographic detection. The composite nano-label can be used for simultaneously detecting anti-coronavirus IgM and IgG in a clinicalblood sample in a short time, has ultrahigh sensitivity, 100% accuracy degree, good dispersity, high stability and easy preparation on a large scale, and can be used for capturing, enriching and quickly detecting target substances in a complex sample system.

Description

technical field [0001] The invention relates to the field of novel nanomaterials and surface-enhanced Raman scattering (SERS), in particular to a silicon-core-silver-shell composite nano-label labeled with double-layer Raman molecules, a preparation method and an application of immunochromatography. Background technique [0002] The novel coronavirus (COVID-19) is highly contagious and spreads easily through the air (aerosols, respiratory droplets) and direct contact with body surfaces. Some people with COVID-19 are asymptomatic but still contagious. So far, there are very few vaccines and drugs that can prevent or treat COVID-19, so timely and accurate diagnosis of suspected cases is the most effective way to control the epidemic. Common diagnostic techniques such as real-time reverse transcription-polymerase chain reaction (RT-PCR) and computed tomography have been applied to the clinical diagnosis of COVID-19, and they have good accuracy and reliability for critically il...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N33/543G01N33/558G01N33/532G01N33/58
CPCG01N21/658G01N33/54346G01N33/558G01N33/532G01N33/587
Inventor 王升启肖瑞汪崇文刘晓娴刘海峰
Owner ACADEMY OF MILITARY MEDICAL SCI
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