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Culture medium for cymbidium goeringii rhizome tissue culture and tissue culture method

A tissue culture and rhizome technology, applied in the field of plant cell engineering, can solve problems such as low reproduction efficiency and long tissue culture cycle of Chunlan, and achieve the effects of rapid proliferation, promotion of rapid differentiation into buds, and prevention of browning

Active Publication Date: 2021-02-09
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide the culture medium that is used for Chunlan rhizome tissue culture, has overcome the technical problem that Chunlan tissue culture period is long and reproductive efficiency is low

Method used

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  • Culture medium for cymbidium goeringii rhizome tissue culture and tissue culture method
  • Culture medium for cymbidium goeringii rhizome tissue culture and tissue culture method
  • Culture medium for cymbidium goeringii rhizome tissue culture and tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]Example 1: Preparation of culture medium

[0032]A proliferation medium and a differentiation medium were prepared, which were used for the experiment of proliferation of Chunlan rhizome and the experiment of differentiation of Chunlan rhizome respectively.

[0033]The basic proliferation medium is MS medium (pH 5.4) containing basic proliferation components, and the proliferation promotion components and their contents are 1.5 mg·L.-1 6-BA (6-benzylaminopurine), 5.0mg·L-1NAA (naphthalene acetic acid), 30.0g·L-1Sucrose, 7.0g·L-1Agar, 2.0g·L-1Activated carbon.

[0034]The basic differentiation medium is MS medium (pH 5.4) containing basic differentiation components, and the growth promoting components and their contents are: 5mg·L-1 6-BA, 0.2mg·L-1NAA, 20.0g·L-1Sucrose, 7.0g·L-1Agar.

[0035]The above medium was separately added to filter sterilized and the concentration was 0.2mmol·L-1, 0.5mmol·L-1, 1.0mmol·L-1, 2.0mmol·L-1The 20 amino acids (respectively glycine, alanine, valine, leucine...

Embodiment 2

[0044]Example 2: Chunlan rhizome proliferation experiment

[0045]Prepare a sterile, well-growing, branched rhizome with a diameter of about 2mm, cut into 0.5cm length for later use. Transfer 0.5cm length of Chunlan rhizomes into the propagation medium, 1g per bottle, wipe off the water and medium with sterile paper towels from the rhizome material, and then evenly inoculate the medium (proliferation medium to be tested) after weighing Surface, 25℃, light 2000Lx, 12 hours / day. After 60 days (in actual operation, it can be cultured for 60-90 days according to the actual situation) after taking out the rhizomes in the bottle to wipe the water and residual medium, then weigh them, record the weight of each rhizome on the medium, and count the rhizomes Stem browning.

[0046]Preparation of proliferation medium to be tested (take 1L as an example): Take about 300ml of distilled water and heat, when small bubbles appear, add 7g of agar, stir evenly, after the dissolution is complete, add 30g of...

Embodiment 3

[0047]Example 3: Chunlan rhizome differentiation experiment

[0048]The rhizomes of Chunlan with a length of 1 cm (0.5 cm rhizomes from Example 2 were cultured to a length of about 1 cm through the basic proliferation medium) were transferred into differentiation medium, 18 per bottle, and cultured in the culture room. 25°C, 2000Lx light, 12 hours / day. About 15 days, the rhizomes begin to differentiate, about 60 days (in actual operation, can be cultured for 60-90 days according to the actual situation), the small buds grow to 2-3 cm. Record the number of rhizomes on the medium, the number of shoots produced, and count the browning of the rhizomes.

[0049]Preparation of differentiation medium to be tested (take 1L as an example): Take about 300ml of distilled water and heat, when small bubbles appear, add 7g of agar, stir evenly, and after dissolution is complete, add 30g of sucrose and 2.37g of MS medium powder, and stir. To dissolve completely, add plant growth regulator 1mg·ml-1 6-BA ...

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Abstract

The invention belongs to the field of plant cell engineering, and particularly relates to a culture medium for cymbidium goeringii rhizome tissue culture and a tissue culture method. The culture medium for cymbidium goeringii rhizome tissue culture comprises a differentiation culture medium, the differentiation culture medium is a basic differentiation culture medium added with differentiation promoting amino acid, and the differentiation promoting amino acid comprises asparagine. According to the scheme, the technical problems of long cymbidium goeringii tissue culture period and low propagation efficiency are solved. According to the scheme, the vacancy of influences of the amino acid on cymbidium goeringii growth can be filled up, tissue culture and rapid propagation can be directly guided, reference is provided for industrial seedling raising, and important theoretical significance and practical value are achieved.

Description

Technical field[0001]The invention belongs to the field of plant cell engineering, and specifically relates to a culture medium and a tissue culture method for cultivating Chunlan rhizome tissue.Background technique[0002]Cymbidium goeringii belongs to the ground species in the genus Orchidaceae. Spring orchids are rich in fragrance, elegant in color, beautiful in flower posture, and elegant in leaves. They are a unique species in the huge orchid family and one of the main groups of orchids in my country. It is widely distributed, rich in resources, and has a long history of cultivation. Chunlan has extremely high ornamental value, medicinal value and collection value, so the market demand continues to expand. In recent years, due to over-excavation and habitat destruction, Chunlan's wild resources have declined sharply, and demand has been in short supply.[0003]In order to protect the wild resources of Chunlan and meet market demand, it is imperative to realize the rapid propagation...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H6/62
CPCA01H4/001A01H4/008A01H6/62
Inventor 黄玮婷方中明黎钟懋王思马红春
Owner GUIZHOU UNIV
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