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Bio-based self-repairing hydrogel as well as preparation method and application thereof

A self-healing, bio-based technology, applied in medical science, bandages, etc., can solve the problems of weak shape plasticity, poor self-healing performance, and insufficient mildness of hydrogel cross-linking, achieving thermodynamic reversibility, easy operation, The effect of promoting wound healing

Pending Publication Date: 2021-02-09
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of the existing hydrogel cross-linking methods not being gentle enough, weak shape plasticity, and poor self-healing performance, the present invention provides a bio-based self-healing hydrogel and its preparation method and application

Method used

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  • Bio-based self-repairing hydrogel as well as preparation method and application thereof
  • Bio-based self-repairing hydrogel as well as preparation method and application thereof
  • Bio-based self-repairing hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] (1) Dissolve γ-polyglutamic acid (700kDa) in deionized water, the mass concentration of γ-PGA is 10g / L, stir and mix evenly to make a solution with pH=4; then add 1-(3-di Methylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), stirred and activated at 18°C ​​for 30min. Add cysteine ​​hydrochloride (Cys·HCl), and stir at room temperature for 18 hours; the molar ratio of each substance is as follows, EDC:γ-PGA(-COOH)=1:1, EDC:NHS=1:1, γ- PGA(-COOH):Cys·HCl=1:1. The obtained system was transferred to a dialysis bag, and placed in deionized water for dialysis for 3 days; the purified solution obtained after dialysis was freeze-dried to obtain a cysteine ​​molecule-modified γ-polyglutamic acid polymer (γ-PGA-SH ), the grafting rate of Cys was 25%.

[0065] (2) Dissolve hyaluronic acid (1000kDa) in deionized water, the mass concentration of HA is 10g / L, stir and mix evenly; then add sodium periodate aqueous solution (1mol / L ) After reacti...

Embodiment 2

[0068] (1) Dissolve γ-polyglutamic acid (γ-PGA, molecular weight is 1 million Daltons) in deionized water to make a solution of pH=6, the mass concentration of γ-PGA is 15g / L, stir and mix Uniform; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), stir and activate at room temperature for 60min . Add cysteine ​​hydrochloride (Cys·HCl), and stir at room temperature for 25 hours; the molar ratio of each substance is as follows, EDC:γ-PGA(-COOH)=1.5:1, EDC:NHS=1.5:1, γ- PGA(-COOH):Cys·HCl=1:1. The obtained system was transferred to a dialysis bag, and placed in deionized water for dialysis for 3 days; the purified solution obtained after dialysis was freeze-dried to obtain a cysteine ​​molecule-modified γ-polyglutamic acid polymer (γ-PGA-SH ), the grafting rate of Cys was 31%.

[0069] (2) Dissolve hyaluronic acid (1000kDa) in deionized water, the mass concentration of HA is 10g / L, stir and mix evenly; then add sodium pe...

Embodiment 3

[0072] (1) Dissolve γ-polyglutamic acid (γ-PGA, molecular weight 100,000 Daltons) in MES buffer (pH=6.0, 0.2M), the mass concentration of γ-PGA is 30g / L, stir and mix Uniform; then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), stir and activate at 37°C 120min. Add cysteine ​​hydrochloride (Cys·HCl), and stir at room temperature for 32 hours; the molar ratio of each substance is as follows, EDC:γ-PGA(-COOH)=3:1, EDC:NHS=2:1, γ- PGA(-COOH):Cys·HCl=1:1.5. The obtained system was transferred to a dialysis bag, placed in deionized water and dialyzed for 7 days; the obtained purified solution after dialysis was freeze-dried to obtain a cysteine ​​molecule-modified γ-polyglutamic acid polymer (γ-PGA-SH ), the grafting rate of Cys was 43%.

[0073] (2) Dissolve hyaluronic acid (300kDa) in deionized water, the mass concentration of HA is 30g / L, stir and mix evenly; then add sodium periodate aqueous solution (0.5mol / L) After r...

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Abstract

The invention discloses bio-based self-repairing hydrogel as well as a preparation method and application thereof. The preparation method comprises the step of mixing cysteine-modified gamma-polyglutamic acid and an aldehyde hyaluronic acid polymer in water or a buffer solution to form the bio-based self-repairing hydrogel. The dynamic covalent cross-linked hydrogel is constructed in a physiological pH environment through a thiol-aldehyde addition reaction between sulfydryl groups and aldehyde groups on cysteine molecules, has the advantages of self-adaptability, self-healing property, biodegradability and the like, and is convenient to operate, free to form, easy to inject and expected to be applied to the fields of medical dressings, 3D printing, tissue engineering and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a bio-based self-repairing hydrogel, a preparation method thereof and an application in biomedicine. Specifically, it is an injectable self-healing hydrogel obtained by dynamic covalent cross-linking of cysteine-modified γ-polyglutamic acid and formylated hyaluronic acid. Background technique [0002] Hydrogels are attractive biomaterials that are promising in many ways, especially in the fields of tissue engineering and regenerative medicine. The polymer network of dynamically covalently cross-linked hydrogels is self-adaptive and self-healing, and can be repaired spontaneously after being damaged, which has become a research hotspot in the field of medical materials. [0003] At present, the formation methods of hydrogel materials mainly include four methods: chemical crosslinking, radiation crosslinking, photoinitiated polymerization and physical crosslinking. ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L77/04C08L5/08C08G69/48C08B37/08A61L26/00
CPCC08J3/075C08G69/48C08B37/0072A61L26/0052A61L26/009A61L26/0061A61L26/008C08J2377/04C08J2305/08C08L77/04C08L5/08
Inventor 迟波杨荣徐虹
Owner NANJING UNIV OF TECH
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