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Method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation

A linoleic acid triglyceride, microbial fermentation technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of insurmountable technical barriers to acyl shift, high cost, and high enzyme prices. Avoid the effects of expensive raw materials, low cost and simple process

Active Publication Date: 2021-02-09
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of these two enzymatic methods for preparing OPL is relatively high, and the price of the enzyme is high. In addition, the method also has the technical barrier of acyl group displacement that cannot be overcome.

Method used

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  • Method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation
  • Method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation
  • Method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation

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Experimental program
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Effect test

Embodiment 1

[0031] A method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation, the specific steps are as follows: 1. Seed cultivation

[0032] The glycerol tube strain Rhodococcus opacus PD630 (Rhodococcus opacus PD630, DSMZ44193) frozen in the refrigerator at -80°C——purchased from Beijing Beina Chuanglian Biotechnology Research Institute, was inserted into the nutrient broth medium, and the temperature was 30°C , Cultivate on a shaker with a rotating speed of 160r / min for 24h, which is the seed solution.

[0033] 2. Fermentation culture

[0034] Fermentation Medium Formula KH 2 PO 4 1.5g / L, Na 2 HPO 4 12H 2 O 9g / L, NH 4 Cl 1.07g / L, FeNa·EDTA 5mg / L, MgSO 4 ·7H 2 O 0.5g / L, CaCl 2 2H 2 O 20mg / L, H 3 BO 3 0.3mg / L, Na 2 MoO 4 2H 2 O2mg / L, ZnSO 4 ·7H 2 O 0.1mg / L, CoCl 2 ·6H 2 O 0.2mg / L, MnCl 2 4H 2 O 0.05mg / L, NiCl 2 ·6H 2 O0.02mg / L, CuCl 2 2H 2O 0.01mg / L, ethyl oleate, ethyl palmitate and ethyl linoleate = 1:1.2:2 (n / n...

Embodiment 2

[0051] First seed culture, according to the formulation of fermentation medium (wherein the carbon source is ethyl oleate: ethyl palmitate: ethyl linoleate = 1:1:1n / n / n) for fermentation culture, according to 1% Inoculum Amount The seed solution was inoculated into a 250mL Erlenmeyer flask containing 100mL of fermentation medium, and cultured on a shaking table at 30°C and 160r / min for 4 days. The bacteria were obtained by centrifugation, and the dry cell weight, oil content, and fatty acid composition of the whole sample were determined according to the above-mentioned method.

Embodiment 3

[0053] First seed culture, according to the formulation of the fermentation medium (wherein the carbon source is ethyl oleate: ethyl palmitate: ethyl linoleate = 1:1.5:2n / n / n) for fermentation culture, according to 1% Inoculum Amount The seed solution was inoculated into a 250mL Erlenmeyer flask containing 100mL of fermentation medium, and cultured on a shaking table at 30°C and 160r / min for 4 days. The bacteria were obtained by centrifugation, and the dry cell weight, oil content, and fatty acid composition of the whole sample were determined according to the above-mentioned method.

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Abstract

The invention discloses a method for preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride by microbial fermentation. The method is characterized in that rhodococcus opacus is inoculatedinto a fermentation medium for fermentation to obtain a fermentation product, centrifuging is conducted, bacterial cells are collected, the cell walls of the bacterial cells are crushed, and grease is collected to obtain the 1-oleic acid- 2-palmitic acid-3-linoleic acid triglyceride (OPL); and a carbon source of the fermentation culture medium comprises ethyl oleate, ethyl palmitate and ethyl linoleate. According to the method, the problems of expensive raw materials, use limitation and the like in the OPL preparation process are avoided, the method is simple in process, the OPL grease can beobtained only by microbial fermentation, microbial cell wall breaking and oil extraction; the culture medium mainly comprises water and is low in cost; and the content of sn-2 palmitic acid in the produced grease is high and can reach 70.86%.

Description

technical field [0001] The invention relates to the field of breast milk fat substitutes, in particular to a method for fermenting and preparing 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride. Background technique [0002] Fat is an important ingredient in infant formula. Human milk fat is one of the main nutrients in human milk. It can not only provide about 45% of the energy required by infants, but also provide the essential fatty acids required for infant growth and development. Human milk fat has a special fat structure, and its C16:0 is mainly distributed in the sn-2 position, while the C16:0 of ​​mixed vegetable oil is mainly concentrated in the sn-1, 3 positions, which is difficult for infants to absorb and utilize, and is easy to form insoluble calcium The soap is excreted with feces, resulting in a double loss of energy and calcium. It can be seen that it is particularly important to develop a human milk fat substitute whose fatty acid composition and ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/01
CPCC12P7/6472
Inventor 杨继国张林尚楚美云娄文勇
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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