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Method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and application

An aminobiphenyl and hypochlorous acid detection technology, applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of high cost, tissue damage, complicated operation, etc., and achieve high accuracy.

Active Publication Date: 2021-02-09
NANJING UNIV OF INFORMATION SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But on the other hand, studies have shown that excessive hypochlorite can cause tissue damage and diseases, such as neuronal degeneration, cancer, cardiovascular disease, and arthritis. Therefore, the development of rapid, sensitive, and specific detection of hypochlorite ions The method has important research significance in the fields of environmental science, biological science, food health, etc. The traditional methods for detecting hypochlorite include titration, electrochemical methods, etc., but these methods are generally complicated to operate, high in cost, and low in sensitivity

Method used

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  • Method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and application
  • Method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and application
  • Method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and application

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Embodiment

[0020] The method of utilizing tetrakis (4-aminobiphenyl) ethylene to detect hypochlorite of the present embodiment, the specific steps are as follows:

[0021] AIE experiment of tetrakis(4-aminobiphenyl)ethene compound. The specific structural formula of the tetrakis (4-aminobiphenyl) ethylene compound used in this embodiment is as follows Figure 5 shown. Dissolve the above-mentioned tetrakis(4-aminobiphenyl)ethylene compound in tetrahydrofuran (THF) to make a 400µM solution as the mother solution, take ten 10ml glass bottles, numbered 0-9 respectively, and add 1ml of the above mother solution to each bottle , and then add deionized water at the ratio of 0, 10%, 20%...90% respectively, and finally add tetrahydrofuran to make 10ml mixed solutions respectively. Test the fluorescence intensity of each group of mixed solutions on a fluorescence analyzer, such as figure 1 As shown, it is the AIE fluorescence curve of the mixed solution made of the above-mentioned tetrakis (4-a...

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Abstract

The invention discloses a method for detecting hypochlorite by tetra (4-aminobiphenyl) ethylene and application thereof and belong to the technical field of hypochlorite detection analysis. The methodcomprises the following steps of dissolving a tetra (4-aminobiphenyl) ethylene compound in an organic solvent to prepare a mother liquor, and adding tetrahydrofuran and deionized water into the mother liquor to prepare a fluorescent probe for detecting hypochlorite ions. Multiple groups of mother liquor are taken in the preparation process of the fluorescent probe, tetrahydrofuran and deionized water in different proportions are respectively added into the multiple groups of mother liquor to prepare a mixed solution, fluorescence intensity of the mixed solution is tested on a fluorescence analyzer, and the group with the maximum fluorescence intensity is taken as the fluorescent probe for detecting hypochlorite ions. The fluorescent probe prepared by the method has relatively high accuracy in detection of hypochlorite ions.

Description

technical field [0001] The invention relates to a method for detecting hypochlorite by tetrakis(4-aminobiphenyl)ethylene, and belongs to the technical field of detection and analysis of hypochlorite. Background technique [0002] Hypochlorous acid (HOCl), as an important reactive oxygen species, is generally produced by hydrogen peroxide and chloride ions under the catalysis of myeloperoxidase, and usually spontaneously hydrolyzes in a neutral solution at pH 7.0 to form free of hypochlorite ion. On the one hand, hypochlorite ion plays an important physiological role in organisms, participates in many physiological activities, and is closely related to the immune system. But on the other hand, studies have shown that excessive hypochlorite can cause tissue damage and diseases, such as neuronal degeneration, cancer, cardiovascular disease, and arthritis. Therefore, the development of rapid, sensitive, and specific detection of hypochlorite ions The method has important resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06
CPCG01N21/643C09K11/06G01N2021/6432C09K2211/1007
Inventor 吴琴陶涛
Owner NANJING UNIV OF INFORMATION SCI & TECH
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