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Paenibacillus sp. CL01 and application thereof

A technology of Paenibacillus and Bacillus, applied in Paenibacillus CL01 and its application field, can solve the problems of strain biological characteristics, antibacterial spectrum difference, etc., to improve food and ecological environment safety, good economic and social benefits, high antagonistic active effect

Active Publication Date: 2021-02-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, strains from different sources usually have obvious differences in biological characteristics, antibacterial spectrum, etc., and are strain-specific.

Method used

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  • Paenibacillus sp. CL01 and application thereof
  • Paenibacillus sp. CL01 and application thereof
  • Paenibacillus sp. CL01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The isolation and identification of embodiment 1 Paenibacillus CLO1

[0039] 1. Isolation of Paenibacillus CL01

[0040] Collect soil samples from the healthy pear tree rhizosphere soil in pear orchards with serious pear fungal diseases in Jurong City, Jiangsu Province, put 10g soil samples into a 50mL triangular flask equipped with sterile water and sterilized glass beads, 200rpm / min Shake for 30 minutes; take the shaken suspension for serial gradient dilution, 10 3 、10 4 Draw 200 μL of the double-diluted bacterial suspension and spread it evenly on the NA medium plate. After culturing at 28°C for 48 hours, select a single colony with different colony shape and use the method of streak transfer to purify, and transfer the purified strain to Put it on the NA slant medium and store it at 4°C for later use. The strains with good antagonistic effect were further treated with 20% glycerol as a protective agent, suspended and frozen in a -70°C ultra-low temperature refrige...

Embodiment 2

[0052] Example 2: Determination of the optimum growth pH value of Paenibacillus CLO1.

[0053] Take out the CL01 strain stored in the glycerin tube of the ultra-low temperature refrigerator at -70°C, inoculate it on the NA solid plate, and culture it at 28°C for 24 hours; use a sterilized toothpick to pick a single colony of CL01 and inoculate it into 250mL containing 50mL NB liquid medium In an Erlenmeyer flask, cultured at 180r / min and 28°C for 16h as the seed solution; the seed solution was inoculated to the initial pH values ​​of 3.0, 4.0, 5.0, 6.0, 6.5, In the 50mL NB liquid medium of 7.0, 7.5, 8.0, 9.0, cultured at 180r / min, 28°C for 20h, after 20h, the UV-visible spectrophotometer measured the optical density value of the bacterial suspension at 600nm wavelength at different initial pH values. The result is as image 3 As shown, CL01 can grow in the range of initial pH value between 4.0 and 9.0. When the initial pH value is in the range of 6.0 to 7.5, it has a higher o...

Embodiment 3

[0054] Embodiment 3: the preparation of the microbial bacterial agent based on Paenibacillus CLO1

[0055] The microbial bacterial agent used to prevent and treat the main fungal diseases of pears comprises the fermentation broth of Paenibacillus CL01, the filtrate of the fermentation broth and the crude extract of antibacterial active substances of the fermentation broth, and the preparation steps are as follows:

[0056] 1) Activated strain: take the glycerol strain of Paenibacillus CLO1 of the present invention, inoculate it on the NA medium plate by streaking, and culture it at 28°C for 24 hours;

[0057] 2) Preparation of seed solution: Pick a single colony of Paenibacillus CL01 on an NA plate and inoculate it into NB liquid medium, and culture it with shaking at 28°C for 16-24 hours, and the shaking frequency is 150r / min-200r / min;

[0058] 3) Preparation of fermentation broth: Inoculate the seed solution of the bacterial strain CLO1 prepared in step 2) into the NB liquid...

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Abstract

The invention discloses paenibacillus sp. CL01 and an application thereof. The paenibacillus sp. CL01 is preserved in China General Microbiological Culture Collection Center on June 28, 2020, and thepreservation number of the strain is CGMCC No.20161. The strain is wide in antibacterial spectrum and strong in antibacterial ability, has a strong inhibition effect on common pathogenic fungi on peartrees such as colletotrichum gloeosporioides, alternaria alternata, alternaria alternata and valsa ceratosperma, and also has a strong antagonistic effect on phytopathogens such as rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum and phytopathogen. The biocontrol microbial inoculum prepared from the strain has a relatively high biocontrol effect on fungal diseases of fruits and leaves on pear trees. The composition can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, has good economic and social benefits, and has a very wide application prospect in biological prevention and control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Paenibacillus CLO1 and application thereof. Background technique [0002] The pear fruit industry is one of the most important fruit industries in my country and occupies an important position in the agricultural economy. my country's pear production accounts for about 2 / 3 of the world's total output, and its export volume accounts for 1 / 6 of the world's total export volume. The occurrence and harm of pear fungal diseases are one of the important factors limiting the healthy development of pear industry in my country. The diseases that harm pears are mainly fungal diseases, which not only harm branches, stems, leaves, and fruits during the production period, but also cause more serious fruit rot caused by fungi during the fruit ripening, picking and storage periods, which have become a factor that cannot be ignored in reducing production . Pear trees ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/01
CPCA01N63/25C12N1/205C12R2001/01
Inventor 李朝辉刘凤权赵延存孙伟波包艳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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