Transcriptome analysis method and system without reference genome sequence

A technology of transcriptome analysis and reference genome, which is applied in the field of transcriptome sequencing data analysis, can solve the problems of transcriptome analysis without reference genome sequence and achieve high accuracy
CN112397149AActive Publication Date: 2021-02-23TIANJIN MODERN INNOVATIVE TCM TECH CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
TIANJIN MODERN INNOVATIVE TCM TECH CO LTD
Publication Date
2021-02-23

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Abstract

The invention provides a transcriptome analysis method and system without a reference genome sequence. The analysis method comprises the following steps: acquiring second-generation RNA effective sequencing data and third-generation RNA effective sequencing data of a to-be-detected sample; correcting the third-generation RNA effective sequencing data by utilizing the second-generation RNA effective sequencing data; redundancy elimination is carried out on the valid data after the third-generation correction to obtain a unigene sequence; performing sequence comparison on the second-generation RNA effective sequencing data and the unigene sequences, and performing statistics on the reads number on each unigene sequence by utilizing a comparison file to obtain an expression level FPKM value of each gene. The analysis method integrates the advantages of a second-generation sequencing technology and a third-generation sequencing technology, and solves the problem that no transcriptome of areference-free genome sequence is analyzed by utilizing third-generation sequencing data at present.
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Description

technical field

[0001] The present invention relates to the field of transcriptome sequencing data analysis, in particular to a transcriptome analysis method and system without a reference genome sequence. Background technique

[0002] Next-generation sequencing technology refers to the second-generation sequencing technology developed based on the principle of sequencing-by-synthesis. The illumina sequencing platform is a widely used next-generation sequencing technology, which has the advantages of high sequencing throughput, low cost, and accurate sequencing results. The disadvantages are that the sequencing read length is short (below 500bp), and the sample preparation process is cumbersome.

[0003] Three-generation sequencing technology refers to the third-generation sequencing technology developed based on the principle of single-molecule sequencing technology. Nanopore sequencing platform (Oxford Nanopore Technology, ONT) is the third-generation gene sequencing tec...

Claims

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