Detection method for logarithmic growth phase of actinomycete strain producing apramycin

Abstract

The invention discloses a detection method for the logarithmic growth phase of an actinomycete strain producing apramycin, and relates to the technical field of microbial fermentation seed preparation. The detection method comprises the following steps of: S1, strain preservation: preparing a batch of actinomycete seed liquid culture medium and fermentation culture medium, subpackaging the liquidculture medium and the fermentation culture medium into seed shake flasks, setting three parallel experiments, and subpackaging the fermentation culture medium into fermentation shake flasks; and S2,seed culture medium: inoculating the preserved strain into the seed liquid culture medium in the step S1, taking the culture medium which is not inoculated with the strain as a blank control, and carrying out culture under the same condition and synchronizing with seed tank culture. According to the detection method for the logarithmic growth phase of the actinomycete strain producing apramycin, the original measurement technology is combined together and is combined with production experience, so that the defect of a single technology is overcome, the measurement result is more accurate, thetheory and the actual production experience are combined together, the laboratory and the production are tightly combined, and excellent seeds meeting the conditions are provided for production.

Description

Technical field[0001]The invention relates to the technical field of microbial fermentation seed preparation, in particular to a method for detecting the logarithmic growth phase of apramycin-producing actinomycetes.Background technique[0002]In the industrial-scale fermentation production, the quality of seeds plays a key role in fermentation production. Selecting a reasonable seed culture time can provide the fermentation tank with seeds with strong metabolism and sufficient quantity, which shortens the fermentation production cycle and equipment utilization. To improve, the fermentation of actinomycetes is a very complicated process, which is affected by many environmental conditions, and the cultivation time of seeds under various influencing factors is very critical. Studies have shown that the growth cycle of actinomycetes is divided into four stages, the lag phase, the logarithmic growth phase, the stable growth phase and the decay phase. In these four stages, the population c...

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Problems solved by technology

This method not only requires a sufficient amount of seed culture solution, but also has cumbersome steps and is prone to human error in the process

In addition, in actual production, the composition of the seed medium is complex and contains a variety of insoluble substances, which is not easy to clean with sterile water. There are certain differences between the conditions of laboratory shaker flask seed cultivation and the conditions of fermenters, and the state of laboratory seeds cannot fully reflect fermentation. Growth of seeds in pots

In addition, the transplanting time of actinomycetes strains used in industrial production is often determined according to production experience and observation of their growth state, which has certain fluctuations. Therefore, we propose an apramycin-producing actinomycetes A detection method for the logarithmic growth phase of bacterial strains to solve the above problems

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