Ring fiber core beak-shaped optical fiber optical tweezer system with stable capturing function

Abstract

A ring fiber core bird beak-shaped optical fiber optical tweezer system with a stable capturing function is characterized in that optical fiber optical tweezers are composed of a ring fiber core birdbeak-shaped optical fiber probe, a laser device, an adjusting frame, a sample groove, a microscopic imaging system, a Raman laser source and a Raman detector. The bird beak-shaped optical fiber probeis characterized in that evanescent field distribution of the side face of an optical fiber is enhanced by changing the curvature radius of a bent tip area of the optical fiber, gradient force borne by cells is increased, and therefore stable capture of the cells is achieved. Finally, Raman laser is emitted into the bird beak-shaped optical fiber optical tweezers, and corresponding Raman frequencyshift can be acquired through a detector, thereby realizing cell characteristic detection.

Description

[0001] (1) Technical field

[0002] The invention relates to a ring fiber core bird's beak optical fiber optical tweezers system with a stable capturing function, which belongs to the technical field of light manipulation.

[0003] (2) Background technology

[0004] Since the pioneering work of Ashkin et al., optical tweezers have become one of the most important research tools in the field of biological and physical research. Tapered fiber optic tweezers (TOFTs) are an important optical manipulation tool, widely used in the trapping of micro-nano particles and the manipulation of multi-particles, such as the organization of micro-particles and the patterning of cells. In typical TOFTs, fabrication methods such as polishing, thermal drawing, and chemical etching are used to process the fiber tip into a tapered structure. In recent years, a lot of research has been done on the performance of tapered fiber optic tweezers to achieve non-contact trapping, dual particle trapping, a...

Examples

Embodiment 1

[0043] Example 1 In the bird beak-shaped fiber optic probe Y b1 Capturing cells of different particle sizes

[0044] In the system, a laser with a wavelength of 940nm is passed into the ring-core bird’s beak fiber, and the three-dimensional adjustment frame is used to adjust the laser during the transmission process. Observation with a microscope, and finally displayed on a PC through a microscopic imaging system. like Figure 7 shown in Y b1 At , since the gradient force experienced by cells with a particle size of 4 μm is smaller than the scattering force, the resultant force of the two points to the direction of light propagation, so they cannot be trapped at this position, and the capture efficiency ξ is 14.73 PN / W. Similarly, cells with a particle size of 5 μm and 6 μm cannot be captured here, and the capture efficiency ξ is 17.07PN / W and 19.39PN / W.

Embodiment 2

[0045] Embodiment 2 In the bird beak-shaped fiber optic probe Y b2 Capturing cells of different particle sizes

[0046] like Figure 7 shown in Y b2 At , cells with a particle size of 4 μm, 5 μm and 6 μm are subjected to a gradient force that is less than the scattering force, and the resultant force of the two points to the direction of light propagation, so they cannot be captured at this position, and the capture efficiencies ξ are 42.47PN respectively / W, 49.41PN / W and 56.05PN / W.

Embodiment 3

[0047] Example 3 In the bird beak-shaped fiber optic probe Y b3 Capturing cells of different particle sizes

[0048] like Figure 7 shown in Y b3 At , the gradient force on the cells with a particle size of 4 μm is greater than the scattering force on them, and the resultant force of the two points to the focus position of the light field, so the cells can be captured at this position, and the capture efficiency ξ is 104.60PN / W. Similarly, cells with a particle size of 5 μm and 6 μm can also be captured here, and the capture efficiency ξ is 122.44PN / W and 139.31PN / W, respectively.

[0001] (1) Technical field

[0002] The invention relates to a ring fiber core bird's beak optical fiber optical tweezers system with a stable capturing function, which belongs to the technical field of light manipulation.

[0003] (2) Background technology

[0004] Since the pioneering work of Ashkin et al., optical tweezers have become one of the most important research tools in the field of biological and physical research. Tapered fiber optic tweezers (TOFTs) are an important optical manipulation tool, widely used in the trapping of micro-nano particles and the manipulation of multi-particles, such as the organization of micro-particles and the patterning of cells. In typical TOFTs, fabrication methods such as polishing, thermal drawing, and chemical etching are used to process the fiber tip into a tapered structure. In recent years, a lot of research has been done on the performance of tapered fiber optic tweezers to achieve non-contact trapping, dual particle trapping, a...

Examples

Embodiment 1

[0043] Example 1 In the bird beak-shaped fiber optic probe Y b1 Capturing cells of different particle sizes

[0044] In the system, a laser with a wavelength of 940nm is passed into the ring-core bird’s beak fiber, and the three-dimensional adjustment frame is used to adjust the laser during the transmission process. Observation with a microscope, and finally displayed on a PC through a microscopic imaging system. like Figure 7 shown in Y b1 At , since the gradient force experienced by cells with a particle size of 4 μm is smaller than the scattering force, the resultant force of the two points to the direction of light propagation, so they cannot be trapped at this position, and the capture efficiency ξ is 14.73 PN / W. Similarly, cells with a particle size of 5 μm and 6 μm cannot be captured here, and the capture efficiency ξ is 17.07PN / W and 19.39PN / W.

Embodiment 2

[0045] Embodiment 2 In the bird beak-shaped fiber optic probe Y b2 Capturing cells of different particle sizes

[0046] like Figure 7 shown in Y b2 At , cells with a particle size of 4 μm, 5 μm and 6 μm are subjected to a gradient force that is less than the scattering force, and the resultant force of the two points to the direction of light propagation, so they cannot be captured at this position, and the capture efficiencies ξ are 42.47PN respectively / W, 49.41PN / W and 56.05PN / W.

Embodiment 3

[0047] Example 3 In the bird beak-shaped fiber optic probe Y b3 Capturing cells of different particle sizes

[0048] like Figure 7 shown in Y b3 At , the gradient force on the cells with a particle size of 4 μm is greater than the scattering force on them, and the resultant force of the two points to the focus position of the light field, so the cells can be captured at this position, and the capture efficiency ξ is 104.60PN / W. Similarly, cells with a particle size of 5 μm and 6 μm can also be captured here, and the capture efficiency ξ is 122.44PN / W and 139.31PN / W, respectively.

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