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Antifungal peptide and application thereof

An antifungal peptide and antifungal drug technology, applied in the field of medicine, can solve the problems of high cytotoxic antibacterial peptide, high preparation cost, low stability, etc., and achieves inhibition of pathogenic bacteria growth, broad-spectrum antibacterial activity, and good application prospects. Effect

Active Publication Date: 2021-03-02
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Antimicrobial peptides are difficult to produce drug resistance due to the destruction of microbial membranes, and have the potential to become a substitute for antibiotics. However, there are very few antimicrobial peptides that are commercially available or clinically available. In addition to high preparation costs, low efficiency, low stability, and high cell Toxicity is also an obstacle in the practical application of antimicrobial peptides

Method used

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  • Antifungal peptide and application thereof
  • Antifungal peptide and application thereof
  • Antifungal peptide and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] The antibacterial activity research of the At10 of embodiment 1 polypeptide

[0031] 1 Materials and methods

[0032] 1.1 Materials:

[0033] 1.1.1 Strains

[0034] Candida albicans ATCC 10231 was purchased from the American Type Culture Collection, and clinical Candida albicans, clinical Candida tropicalis, and clinical Candida portuguese were donated by Changzhou Second People's Hospital.

[0035] 1.1.2 Drugs and reagents

[0036] Glucose, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium dodecyl sulfate (SDS), methanol (chromatographic grade), acetonitrile (chromatographic grade) were purchased from Sinopharm Group, tryptone and yeast extract were purchased from Angel, agar Powder, 2.5% glutaraldehyde solution, polylysine solution, PI dye, DAPI dye were purchased from Suo Laibao, malt extract, YM solid medium were purchased from Qingdao Haibo Company, DPPC and DPPG were purchased from Aladdin Company , SYTO-9 dye was purchased from Sigma, USA.

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Abstract

The invention discloses an antifungal peptide and application thereof, and belongs to peptide antibiotics, and the antifungal peptide has the following amino acid sequence: At3: KLLKKVKLFKLLK; At5:KIIKKIIKIIKKIIK; At10:IKKIIKIIKKIIKKI. The antibacterial peptide is composed of hydrophilic and oleophylic amino acids, is stable in structure, is randomly curled in water, and is alpha-spiral in a phospholipid simulated membrane environment. The antibacterial peptide has the effect of remarkably inhibiting the growth and propagation of various pathogenic fungi, including candida albicans (ATCC 10231), clinical candida albicans, clinical candida tropicalis and clinical candida utilis. The novel antifungal peptide provided by the invention can effectively replace traditional antibiotics, does noteasily generate drug resistance, and has a good application prospect.

Description

technical field [0001] The invention optimizes and designs a class of novel antibacterial peptides with broad-spectrum antibacterial properties, and the invention belongs to the technical field of medicine. Background technique [0002] The discovery of antibiotics has solved the problem of microbial infection for a long time. However, with the abuse of antibiotics, the resistance of microorganisms to antibiotics has been continuously strengthened, and the cycle has been shortened, resulting in the proliferation of drug-resistant bacteria in recent years, and even super The production of bacteria has a great impact on the safety of human life. Microbial drug resistance is constantly emerging and accelerating, making the effect of traditional antibiotics on microorganisms to decline again and again, and there is an urgent need for new antibiotics to replace traditional antibiotics and put them on the market. [0003] Antimicrobial peptides are a class of small-molecule activ...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61K38/17A61P31/10
CPCC07K14/43563A61P31/10A61K38/00
Inventor 招秀伯唐婧李月平王静芳尤蓉蓉吕松伟
Owner CHANGZHOU UNIV