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Micro-fluidic chip, and device and method for accurate and quantitative micro-fluidic operation of biological sample

A technology for microfluidic chips and biological samples, which is applied in the field of devices for microfluidic manipulation of biological samples, can solve the problem of inability to achieve, accurately and quantitatively control the amount of freezing liquid, and difficult to generate a wide range of gradients across multiple concentrations, etc. question

Active Publication Date: 2021-03-02
苏州摩畅科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether it is the above-mentioned manual scheme or the automatic scheme, it uses the traditional pipette-like dilution method, which usually can only produce a specific concentration gradient of the equilibrium solution and freezing solution, and it is difficult to produce a wide range of gradients across multiple concentrations , there is room for further improvement
In order to minimize the impact of the liquid exchange process on the activity of the cells, the ideal way to exchange the liquid is to generate multiple precise and controllable concentration gradients from low to high, and the current scheme cannot achieve this function
In addition, the current scheme cannot accurately and quantitatively control the amount of freezing liquid that is in contact with biological samples before freezing, and further improvement is needed

Method used

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  • Micro-fluidic chip, and device and method for accurate and quantitative micro-fluidic operation of biological sample
  • Micro-fluidic chip, and device and method for accurate and quantitative micro-fluidic operation of biological sample

Examples

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Embodiment 1

[0067] Embodiment 1 discloses a device for microfluidic manipulation of a single cell sample (referred to as "device"), such as figure 1 As shown in A, the device mainly includes three major components, that is, a microfluidic chip 1, and a pressure applying mechanism 2 and a valve 3 connected thereto. The microfluidic chip 1 can be connected to the pressure applying mechanism 2 and the valve 3 respectively by connecting hoses. figure 1 B shows the working state of the device when it is used to suck the single-cell biological sample 4 with the cell culture solution 6 from the cell culture dish 5 . It should be noted that the single cell in the embodiment includes a single cell and a single cell cluster.

[0068] The microfluidic chip 1 is mainly used for the capture and extrusion of cells, the suction and extrusion of liquids used in various vitrification freeze-thaw processes, and the accurate and controllable digitalization of liquid droplets during the process of suction a...

Embodiment 3

[0099] Embodiment 3 discloses a simplified version of a device for microfluidic manipulation of a single cell sample (referred to as "simplified device"), such as Figure 9 As shown in A, the device mainly includes a microfluidic chip 1 and a pressure applying mechanism 2 connected thereto. The microfluidic chip 1 can be connected with the pressure applying mechanism 2 by connecting a hose. Figure 9 B shows the working state of the device when it is used to suck the single-cell biological sample 4 with the cell culture solution 6 from the cell culture dish 5 . Compared with the device described in Example 1, the main difference is that the simplified device omits the valve 3, and correspondingly, the structure of the microfluidic chip 1 is further simplified, that is, the gas connection connected to the valve 3 is omitted. Channel 15. Embodiment 3 also discloses the simplified structure of this microfluidic chip. Other than that, the structures and functions of the microfl...

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Abstract

The invention relates to a microfluidic chip, and a device and method for accurate and quantitative micro-fluidic operation of a biological sample. The brand-new device and method capable of achievingdigital operation are provided for biological sample micro-flow operation, accurate quantification of liquid suction and removal is achieved for the first time through a digital droplet flow meter integrated in a micro-fluidic chip, and the device is used in cooperation with an external pressure applying mechanism capable of being repeatedly loaded and a valve. The concentration gradient of a liquid around the biological sample can be continuously adjusted in a digital droplet generation mode, so that continuous digital liquid dilution is realized, meanwhile, the amount of the liquid in contact with the biological sample during freezing and thawing can be quantitatively controlled, and the activity of cells and the sample in the freezing and thawing liquid change process of the biologicalsample is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of precise quantitative microfluidic operation for biological samples, and in particular relates to a microfluidic chip, a device and a method for precise quantitative biological sample microfluidic manipulation. Background technique [0002] Cryopreservation of biological samples usually refers to the preservation of living organisms in ultra-low temperature (minus 196 degrees Celsius) liquid nitrogen to maintain their activity after thawing and recovery. Cryopreservation technology is currently widely used in the long-term storage of cells, tissues and organs and has achieved breakthroughs in many fields, such as in the field of assisted reproduction (freezing of eggs, sperm, embryos, etc.) and stem cell freezing. The current technical difficulty of low temperature cryopreservation is that the water inside and outside the cells of biological samples will form ice crystals during the freezing process, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/36C12M1/26C12M1/00
CPCC12M23/16C12M23/28C12M41/32C12M29/26C12M33/04
Inventor 方泽聪潘挺睿
Owner 苏州摩畅科技有限公司
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