Microfluidic chip, device and method for precise quantitative biological sample microfluidic manipulation

A technology for microfluidic chips and biological samples, applied in the field of devices for microfluidic manipulation of biological samples, can solve the problem of inability to accurately and quantitatively control the amount of freezing liquid, difficulty in generating a wide range of gradients across multiple concentrations, inability to achieve, etc. problems, to achieve continuous digital liquid dilution, reduce uncontrollable risks, and improve the effect of activity

Active Publication Date: 2022-03-04
苏州摩畅科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether it is the above-mentioned manual scheme or the automatic scheme, it uses the traditional pipette-like dilution method, which usually can only produce a specific concentration gradient of the equilibrium solution and freezing solution, and it is difficult to produce a wide range of gradients across multiple concentrations , there is room for further improvement
In order to minimize the impact of the liquid exchange process on the activity of the cells, the ideal way to exchange the liquid is to generate multiple precise and controllable concentration gradients from low to high, and the current scheme cannot achieve this function
In addition, the current scheme cannot accurately and quantitatively control the amount of freezing liquid that is in contact with biological samples before freezing, and further improvement is needed

Method used

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  • Microfluidic chip, device and method for precise quantitative biological sample microfluidic manipulation
  • Microfluidic chip, device and method for precise quantitative biological sample microfluidic manipulation
  • Microfluidic chip, device and method for precise quantitative biological sample microfluidic manipulation

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Embodiment 1

[0067] Example 1 discloses a device for a single cell sample microflow operation (referred to as "device"), such as figure 1 A, the apparatus mainly includes three major components, i.e., microfluidic chip 1, and a coiled pressing mechanism 2 and valve 3. The microfluidic chip 1 can be connected to the pressure mechanism 2 and the valve 3 in a manner of connecting the hose. figure 1 B illustrate the operating state of the device for absorbing the single cell biological sample 4 of the cell culture liquid 6 from the cell culture dish 5. It should be noted that the individual cells in the examples include a single cell and a single cell group.

[0068] The microfluidic chip 1 is mainly used for the capture and extrusion of cells, and the absorption and extrusion of liquids used in various glassized freeze-thaw processes, exactly controllable in the process of droplets during the drawing and extruded liquid. The concentration gradient of different liquids is generated around the cell...

Embodiment 3

[0099] Example 3 discloses a device (referred to as "simplified device") A simplified version of a single cell sample for microfluidic operations, such as Figure 9 Indicated by A, the apparatus includes a micro-fluidic chip and the pressing mechanism 2 coupled thereto. The microfluidic chip 1 can be connected with the pressing means 2 is connected by way of a hose. Figure 9 B shows the cell culture with a suction device for a single cell operating state of liquid 4 in a biological sample from the cell culture dish 6 5. The apparatus of Example 1 in comparison with the embodiment, the main difference is that the valve 3 is omitted to simplify the apparatus, and correspondingly, the structure of the microfluidic chip 1 is further simplified, that is, without the gas valve 3 connected to the communication channel 15. Example 3 The same also discloses a simplified structure of such a micro-fluidic chip. In addition, the structure and function of the microfluidic chip 1 and the pressin...

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Abstract

A microfluidic chip, a device and method for microfluidic manipulation of precise quantitative biological samples. The present invention proposes a brand-new digitally operable device and method for the microfluidic operation of biological samples. The digital droplet flowmeter integrated in the microfluidic chip realizes the precise quantification of liquid inhalation and removal for the first time, and can be repeatedly loaded with the external With the use of advanced pressure mechanism and valve, the liquid concentration gradient around the biological sample can be continuously adjusted by means of digital droplet generation, thereby realizing continuous digital liquid dilution, and quantitatively controlling the liquid in contact with the biological sample during freezing and thawing. The amount can greatly improve the activity of cells and samples during the freezing and thawing of biological samples.

Description

Technical field [0001] The present invention belongs to the biological sample for accurate quantitation BACKGROUND microfluidic operations, particularly to a microfluidic chip, accurate quantification devices and methods of operation of a microfluidic biological sample. Background technique [0002] Cryopreservation of biological samples usually refers to activity after the ultra-low temperature (minus 196 degrees Celsius) is stored in liquid nitrogen in vivo, in order to maintain its melting recovery. Cryopreservation technology is currently widely used in cells, tissues and organs of long-term storage and has received a breakthrough in many areas, for example in the field of assisted reproduction (egg, sperm, embryo freezing, etc.) as well as the field of stem cells frozen. Cryopreservation current cell technical difficulties are internal and external moisture biological samples in the course of frozen ice crystals will form, easily lead to cell death. The current solution is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/36C12M1/26C12M1/00
CPCC12M23/16C12M23/28C12M41/32C12M29/26C12M33/04
Inventor 方泽聪潘挺睿
Owner 苏州摩畅科技有限公司
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