Microfluidic chip, device and method for precise quantitative biological sample microfluidic manipulation

A technology for microfluidic chips and biological samples, applied in the field of devices for microfluidic manipulation of biological samples, can solve the problem of inability to accurately and quantitatively control the amount of freezing liquid, difficulty in generating a wide range of gradients across multiple concentrations, inability to achieve, etc. problems, to achieve continuous digital liquid dilution, reduce uncontrollable risks, and improve the effect of activity
CN112430531BActive Publication Date: 2022-03-04苏州摩畅科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
苏州摩畅科技有限公司
Publication Date
2022-03-04

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Abstract

A microfluidic chip, a device and method for microfluidic manipulation of precise quantitative biological samples. The present invention proposes a brand-new digitally operable device and method for the microfluidic operation of biological samples. The digital droplet flowmeter integrated in the microfluidic chip realizes the precise quantification of liquid inhalation and removal for the first time, and can be repeatedly loaded with the external With the use of advanced pressure mechanism and valve, the liquid concentration gradient around the biological sample can be continuously adjusted by means of digital droplet generation, thereby realizing continuous digital liquid dilution, and quantitatively controlling the liquid in contact with the biological sample during freezing and thawing. The amount can greatly improve the activity of cells and samples during the freezing and thawing of biological samples.
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Description

Technical field

[0001] The present invention belongs to the biological sample for accurate quantitation BACKGROUND microfluidic operations, particularly to a microfluidic chip, accurate quantification devices and methods of operation of a microfluidic biological sample. Background technique

[0002] Cryopreservation of biological samples usually refers to activity after the ultra-low temperature (minus 196 degrees Celsius) is stored in liquid nitrogen in vivo, in order to maintain its melting recovery. Cryopreservation technology is currently widely used in cells, tissues and organs of long-term storage and has received a breakthrough in many areas, for example in the field of assisted reproduction (egg, sperm, embryo freezing, etc.) as well as the field of stem cells frozen. Cryopreservation current cell technical difficulties are internal and external moisture biological samples in the course of frozen ice crystals will form, easily lead to cell death. The current solution is a...

Claims

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